Simply Modified Biosensor for the Detection of Human IgG Based on Protein AModified Porous Silicon Interferometer

Abstract

A biosensor has been developed based on induced wavelength shifts in the Fabry-Perot fringes in the visible reflection spectrum of appropriately derivatized thin films of porous silicon semiconductors. Porous silicon (PSi) was generated by an electrochemical etching of silicon wafer using two electrode configurations in aqueous ethanolic HF solution. Porous silicon displayed Fabry-Perot fringe patterns whose reflection maxima varied spatially across the porous silicon. The sensor system studied consisted of a mono layer of porous silicon modified with Protein A. The system was probed with various fragments of an aqueous Human Immunoglobin G (Ig G) analyte. The sensor operated by measurement of the Fabry-Perot fringes in the white light reflection spectrum from the porous silicon layer. Molecular binding was detected as a shift in wavelength of these fringes.

Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer

“…Three dimensional (3D) materials ( e.g. , sol–gels, nanoporous hydrogels, nanotextured zinc oxide, carbon nanotube) are widely applied in molecular sensing due to the increased effective surface area, which in turn offers a large number of binding sites. − In addition, diffusion path length is efficiently decreased for antibodies and antigens in 3D structures . For instance, nanoporous glasses (NPGs) have proven effective for enzyme immobilization in biocatalysis. − However, few reports describing the use of NPG as an ELISA substrate have been found.…”