Simply the Best?

Greenwood, Iain A.

doi:10.1161/circresaha.108.186478

Circulation Research

2008

DOI: 10.1161/circresaha.108.186478

|View full text |Cite

Simply the Best?

Iain A. Greenwood

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2011

2015

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the molecular identity of CaCCs is still not known. Several candidates have been proposed including bestrophins (BEST; Leblanc et al, 2005;Greenwood, 2008), CLCA (Leblanc et al, 2005) and the anoctamin (ANO or TMEM) family of proteins (Caputo et al, 2008;Scudieri et al, 2012;Pedemonte and Galietta, 2014). A study by Bernestein and co-workers showed that TMEM channels are important mediators of murine uterine smooth muscle contractility (Bernstein et al, 2014).…”

mentioning

confidence: 99%

Chloride channels mediate sodium sulphide‐induced relaxation in rat uteri

Mijušković

Nikolić‐Kokić

Dušić

et al. 2015

British J Pharmacology

View full text Add to dashboard Cite

BACKGROUND AND PURPOSEHydrogen sulphide reduces uterine contractility and is of potential interest as a treatment for uterine disorders. The aim of this study was to explore the mechanism of sodium sulphide (Na2S)-induced relaxation of rat uterus, investigate the importance of redox effects and ion channel-mediated mechanisms, and any interactions between these two mechanisms. EXPERIMENTAL APPROACHOrgan bath studies were employed to assess the pharmacological effects of Na2S in uterine strips by exposing them to Na2S with or without Cl − channel blockers (DIDS, NFA, IAA-94, T16Ainh-A01, TA), raised KCl (15 and 75 mM), K + channel inhibitors (glibenclamide, TEA, 4-AP), L-type Ca 2+ channel activator (S-Bay K 8644), propranolol and methylene blue. The activities of antioxidant enzymes were measured in homogenates of treated uteri. The expression of bestrophin channel 1 (BEST-1) was determined by Western blotting and RT-PCR. KEY RESULTSNa2S caused concentration-dependent reversible relaxation of spontaneously active and calcium-treated uteri, affecting both amplitude and frequency of contractions. Uteri exposed to 75 mM KCl were less sensitive to Na2S compared with uteri in 15 mM KCl. Na2S-induced relaxations were abolished by DIDS, but unaffected by other modulators or by the absence of extracellular HCO3 − , suggesting the involvement of chloride ion channels. Na2S in combination with different modulators provoked specific changes in the anti-oxidant profiles of uteri. The expression of BEST-1, both mRNA and protein, was demonstrated in rat uteri. CONCLUSIONS AND IMPLICATIONSThe relaxant effects of Na2S in rat uteri are mediated mainly via a DIDS-sensitive Cl − -pathway. Components of the relaxation are redox-and Ca 2+ -dependent.Abbreviations ANO (TMEM16), anoctamin chloride channels; BEST, bestrophin chloride channels; CaCCs, calcium-activated chloride channels; CAT, catalase; GR, glutathione reductase; GSH-Px, glutathione peroxidase; KATP, ATP-sensitive K + channel; NFA, niflumic acid; ROS, reactive oxygen species IntroductionThe smooth muscle layer of the uterus, the myometrium, undergoes profound remodelling that allows rapid changes to occur in different physiological (and pathophysiological) conditions. In a non-pregnant state, myometrial activity is at its highest level during oestrus (Crane and Martin, 1991). Abnormal contractility can underlie disorders such as dysmenorrhea and endometriosis. Several potential inhibitors of myometrial activity have been identified: CGRP (Anouar et al., 1998), NO (Buxton et al., 2001) and carbon monoxide (CO; Acevedo and Ahmed, 1998). Endogenously produced hydrogen sulphide (H2S), which, together with NO and CO, is a recognized gasotransmitter, also appears to be a signalling molecule in rat uterus. Both enzymes responsible for its endogenous production (cystathionine β-synthase and cystathionine γ-lyase) were identified in all rat intrauterine tissues (Patel et al., 2009). Hydrogen sulphide is a potent inhibitor of spontaneous contractions of the pregnant myometrium ...

show abstract

mentioning

confidence: 99%

Chloride channels mediate sodium sulphide‐induced relaxation in rat uteri

Mijušković

Nikolić‐Kokić

Dušić

et al. 2015

British J Pharmacology

View full text Add to dashboard Cite

show abstract

Bestrophin is important for the rhythmic but not the tonic contraction in rat mesenteric small arteries

Broegger

Jacobsen

Dam

et al. 2011

Cardiovascular Research

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Simply the Best?

Cited by 2 publications

References 10 publications

Chloride channels mediate sodium sulphide‐induced relaxation in rat uteri

Chloride channels mediate sodium sulphide‐induced relaxation in rat uteri

Bestrophin is important for the rhythmic but not the tonic contraction in rat mesenteric small arteries

Contact Info

Product

Resources

About