Simulação Do Processo De Produção De Uma Pizzaria

Magalhães, Julia Camila Melo; Magalhães, Marcelo dos Santos

doi:10.22533/at.ed.99418091228

Cited by 1 publication

(1 citation statement)

References 7 publications

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“…Moreover, rather than commencing the time-consuming and expensive in vitro/in vivo experiments directly, using the immunoinformatics analysis that is favorable to formulate the universal MPXV mRNA vaccine and screen the multi-epitope construct containing conserved elements using in silico approaches seems very reasonable. Indeed, a line of research has been dedicated to developing vaccines for COVID-19 [41,88], influenza [89][90][91], and other viruses [92][93][94] using bioinformatics approaches. Until now, there is no study focusing on designing a multi-epitope universal mRNA vaccine against MPXV, VARV, and VACV together.…”

Section: Discussionmentioning

confidence: 99%

Development of a Multi-Epitope Universal mRNA Vaccine Candidate for Monkeypox, Smallpox, and Vaccinia Viruses: Design and In Silico Analyses

Rcheulishvili

Mao

Papukashvili

et al. 2023

Viruses

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Notwithstanding the presence of a smallpox vaccine that is effective against monkeypox (mpox), developing a universal vaccine candidate against monkeypox virus (MPXV) is highly required as the mpox multi-country outbreak has increased global concern. MPXV, along with variola virus (VARV) and vaccinia virus (VACV), belongs to the Orthopoxvirus genus. Due to the genetic similarity of antigens in this study, we have designed a potentially universal mRNA vaccine based on conserved epitopes that are specific to these three viruses. In order to design a potentially universal mRNA vaccine, antigens A29, A30, A35, B6, and M1 were selected. The conserved sequences among the three viral species—MPXV, VACV, and VARV—were detected, and B and T cell epitopes containing the conserved elements were used for the design of the multi-epitope mRNA construct. Immunoinformatics analyses demonstrated the stability of the vaccine construct and optimal binding to MHC molecules. Humoral and cellular immune responses were induced by immune simulation analyses. Eventually, based on in silico analysis, the universal mRNA multi-epitope vaccine candidate designed in this study may have a potential protection against MPXV, VARV, and VACV that will contribute to the advancement of prevention strategies for unpredictable pandemics.

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