Simulated body fluid and the novel bioactive materials derived from it

Abstract

Professor Larry Hench first reported that certain glasses are able to spontaneously bond to living bone in 1970. This discovery stimulated research into new kinds of bone‐bonding materials. However, there were no guiding principles for this purpose, and many animals were sacrificed in the effort to establish them. The present authors proposed in 1991 that the bone‐bonding capacity of a material could be evaluated by examining apatite formation on its surface in an acellular simulated body fluid (SBF), without the need of performing any animal experiments. Various kinds of novel bone‐bonding bioactive materials based on Ti metal and its alloys with a number of different functions have been developed using SBF. Some of these have entered clinical use as important bone‐repairing materials. Without the method of SBF evaluation, these novel materials would not have been developed. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 968–977, 2019.

Antibacterial Properties of Triethoxysilylpropyl Succinic Anhydride Silane (TESPSA) on Titanium Dental Implants

“…In order to provide hydroxyl groups onto dental implants and to ensure optimal silanization, the TESPSA immobilization protocol started with an activation of the samples by alkaline etching [42]. Scanning electron microscopy (SEM) displayed a stable amorphous titanate layer with a nanoporous surface morphology (Ti-N) originating from the activation process, as reported previously [43][44][45] (Figure 2a(ii)). However, the TESPSA immobilization onto the activated surface (Ti-N-TSP) did not reveal any additional differences in the SEM analysis (image not shown).…”

Enhancement of cell differentiation on a surface potential-controlled nitrogen-doped TiO₂ surface

“…This result was not consistent with Ca and P detection by XPS measurement previously reported. 27) These results indicate that adhesive-proteins such as Fn adsorb on Ti surfaces irrespective of surface treatment or not, whereas Ca and P ions adsorb only on negativelyor positively-charged surfaces. Therefore, Fn directly adsorbed onto untreated Ti.…”

Synthesis of borate modified bioactive glass scaffold using PVP burning-out method for bone tissue replacement

“…In vitro bioactivity evaluation. In vitro bioactivity test was assessed by exposing the modified glass samples to the simulated body fluid (SBF) performed according to the recipe reported by Kokubo et al [17,18] and remembered in the numerous published articles, for example Ref. [19,20].…”

“…SBF solution was prepared by mixing the appropriate amounts of sodium chloride (NaCl), sodium hydrogen carbonate (NaHCO 3 ), potassium chloride (KCl), di-potassium hydrogen phosphate trihydrate (K 2 HPO 4 ·3H 2 O) and magnesium chloride hexahydrate (MgCl 2 · 6H 2 O), calcium chloride (CaCl 2 ), sodium sulfate (Na 2 SO 4 ) in deionized water with the help of magnetic stirrer in a beaker, according to the concentrations given in Ref. [17][18][19][20]. Then, the solution was buffered to pH = 7.4 with tris-hydroxymethyl-amino-methane [(CH 2 OH) 3 CNH 3 ] and one molar-hydrochloric acid (1M-HCl).…”