Simulated gastrointestinal digestion of camel and bovine casein hydrolysates: Identification and characterization of novel anti-diabetic bioactive peptides

Mudgil, Priti; Kamal, Hina; Kilari, Bhanu Priya; Salim, Mohd Adam Salim Mohd; Gan, Chee‐Yuen; Maqsood, Sajid

doi:10.1016/j.foodchem.2021.129374

Cited by 74 publications

(26 citation statements)

References 56 publications

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“…MEE exhibited the best scavenging of DPPH (47%) and FGKG displayed the best AAM (54%) and AG suppression (56%). Finally, Mudgil et al (2021) performed the simulated GID of bovine casein (BC) and camel casein (CC) hydrolysates with novel antidiabetic BAPs discovered and characterized. BC and CC hydrolysates were investigated for in vitro antidiabetic effects through AA, AG, and DPP-IV suppression.…”

Section: Antidiabetic Potential Of Bapsmentioning

confidence: 99%

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Patil

Usman

Zhang

et al. 2022

Comp Rev Food Sci Food Safe

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Food‐derived bioactive peptides (BAPs) are recently utilized as functional food raw materials owing to their potential health benefits. Although there is a huge amount of scientific research about BAPs’ identification, purification, characterization, and physiological functions, and subsequently, many BAPs have been marketed, there is a paucity of review on the regulatory requirements, bioavailability, and safety of BAPs. Thus, this review focuses on the toxic peptides that could arise from their primary proteins throughout protein extraction, protein pretreatment, and BAPs’ formulation. Also, the influences of BAPs’ length and administration dosage on safety are summarized. Lastly, the challenges and possibilities in BAPs’ bioavailability and regulatory requirements in different countries were also presented. Results revealed that the human studies of BAPs are essential for approvals as healthy food and to prevent the consumers from misinformation and false promises. The BAPs that escape the gastrointestinal tract epithelium and move to the stomach are considered good peptides and get circulated into the blood using different pathways. In addition, the hydrophobicity, net charge, molecular size, length, amino acids composition/sequences, and structural characteristics of BAPs are critical for bioavailability, and appropriate food‐grade carriers can enhance it. The abovementioned features are also vital to optimize the solubility, water holding capacity, emulsifying ability, and foaming property of BAPs in food products. In the case of safety, the possible allergenic and toxic peptides often exhibit physiological functions and could be produced during the hydrolysis of food proteins. It was also noted that the production of iso‐peptides bonds and undesirable Maillard reaction might occur during protein extraction, sample pretreatments, and peptide synthesis.

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Section: Antidiabetic Potential Of Bapsmentioning

confidence: 99%

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Patil

Usman

Zhang

et al. 2022

Comp Rev Food Sci Food Safe

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“…In dairy, milk proteins are the potent source of bioactive peptides which exert various biological functions, i.e., antioxidant, antimicrobial, anticancer, and anti-hypertensive factors ( 29 , 30 ). As cited in Table 1 , many researchers have assessed the biological activities of bioactive peptides from various milk sources, including camel and bovine casein hydrolyzates ( 31 ), buffalo casein ( 32 ), camel whey protein hydrolyzate ( 33 ), camel milk lactoferrin ( 34 ), goat milk ( 35 ), yak milk ( 36 ), goat milk ( 37 ), buffalo milk ( 38 ), skim milk ( 6 ) camel milk ( 39 ), whey protein hydrolyzate ( 40 ), UHT treated milk ( 41 ), and milk and dairy products ( 42 ) by using various microorganisms and microbial enzymes for proteolysis. The bioactive peptides are liberated during gastrointestinal digestion ( in vivo ), milk products’ manufacturing, and proteolysis ( in vitro ).…”

Section: Milk and Fermented Dairy Products: Source Of Bioactive Peptidesmentioning

confidence: 99%

Conventional and Novel Technologies in the Production of Dairy Bioactive Peptides

et al. 2022

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BackgroundIn recent years, researchers have focused on functional ingredients, functional foods, and nutraceuticals due to the rapidly increasing interest in bioactive components, especially in bioactive peptides. Dairy proteins are a rich and balanced source of amino acids and their derived bioactive peptides, which possess biological and physiological properties. In the dairy industry, microbial fermentation and enzymatic hydrolysis are promising methods for producing bioactive peptides because of their rapid efficiency, and mild reaction conditions. However, these methods utilize less raw material, take long reaction time, result in low yields, and low activity products when used alone, which pose industry to seek for novel methods as pretreatments to increase the yield of bioactive peptides.Scope and ApproachThis review emphasizes the production of peptides from the dairy proteins and discusses the potential use of novel technologies as pretreatments to conventional methods of bioactive peptides production from dairy proteins, including the mechanisms of novel technologies along with respective examples of use, advantages, limitations, and challenges to each technology.Key Findings and ConclusionNoteworthily, hydrolysis of dairy proteins liberate wide-range of peptides that possess remarkable biological functions to maintain human health. Novel technologies in the dairy industry such as ultrasound-assisted processing (UAP), microwave-assisted processing (MAP), and high pressure processing (HPP) are innovative and environmentally friendly. Generally, novel technologies are less effectual compared to conventional methods, therefore used in combination with fermentation and enzymatic hydrolysis, and are promising pretreatments to modify peptides’ profile, improve the yields, and high liberation of bioactive peptides as compared to conventional technologies. UAP is an innovative and most efficient technology as its mechanical effects and cavitation change the protein conformation, increase the biological activities of enzymes, and enhance enzymatic hydrolysis reaction rate.

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“…Values near 1 indicate peptides with high likelihood to be bioactive. Therefore, if the probabilities are ≥0.8, the peptide is said to be predicted as bioactive [34].…”

Section: In Silico Analysis Of Identified Peptides For Their Potentia...mentioning

confidence: 99%

Multifunctional Analysis of Chia Seed (Salvia hispanica L.) Bioactive Peptides Using Peptidomics and Molecular Dynamics Simulations Approaches

Aguilar-Toalá

Vidal-Limón

Liceaga

2022

IJMS

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Chia seed peptides (CSP) can be a source of multifunctional biopeptides to treat non-communicable diseases. However, interactions and binding affinity involved in targeting specific receptors remains unexplored. In this study, molecular simulation techniques were used as virtual screening of CSP to determine drug-like candidates using a multi-target-directed ligand approach. CSP fraction with the best bioactivities in vitro was sequenced. Then, a prediction model was built using physicochemical descriptors (hydrophobicity, hydrophilicity, intestinal stability, antiangiogenic, antihypertensive, and anti-inflammatory) to calculate potential scores and rank possible biopeptides. Furthermore, molecular dynamics simulations (MDS) and ensemble molecular docking analysis were carried out using four human protein targets (ACE, angiotensin converting enzyme; VEGF, vascular endothelial growth factor; GLUC, glucocorticoid and MINC, mineralocorticoid receptors). Five known-sequence peptides (NNVFYPF, FNIVFPG, SRPWPIDY, QLQRWFR, GSRFDWTR) and five de novo peptides (DFKF, DLRF, FKAF, FRSF, QFRF) had the lowest energy score and higher affinity for ACE and VEGF. The therapeutic effects of these selected peptides can be related to the inhibition of the enzymes involved in angiogenesis and hypertension, due to formation of stable complexes with VEGF and ACE binding sites, respectively. The application of MDS is a good resource for identifying bioactive peptides for future experimental validation.

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Simulated gastrointestinal digestion of camel and bovine casein hydrolysates: Identification and characterization of novel anti-diabetic bioactive peptides

Cited by 74 publications

References 56 publications

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Conventional and Novel Technologies in the Production of Dairy Bioactive Peptides

Multifunctional Analysis of Chia Seed (Salvia hispanica L.) Bioactive Peptides Using Peptidomics and Molecular Dynamics Simulations Approaches

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