Simulated moving bed technology with a simplified approach for protein purification

Andersson, Jonatan; Mattìasson, Bo

doi:10.1016/j.chroma.2005.12.018

Cited by 77 publications

(22 citation statements)

References 29 publications

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“…It is a basic protein with isoelectric point of 8.0 to 8.5 consisting of single polypeptide chain of molecular weight of about 80-85 kDa. The anti-bacterial properties of LPO and it is generally recognised as safe status has been published in literature demonstrating its potential to be used as a natural food preservative [1][2][3][4][5]. The most widely recommended industrial application of the lactoperoxidase system in food processing is in the dairy industry.…”

mentioning

confidence: 99%

Integrated Downstream Processing of Lactoperoxidase from Milk Whey Involving Aqueous Two-Phase Extraction and Ultrasound-Assisted Ultrafiltration

Nandini

Rastogi

2010

Appl Biochem Biotechnol

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The present work involves the adoption of an integrated approach for the purification of lactoperoxidase from milk whey by coupling aqueous two-phase extraction (ATPE) with ultrasound-assisted ultrafiltration. The effect of system parameters of ATPE such as type of phase system, polyethylene glycol (PEG) molecular mass, system pH, tie line length and phase volume ratio was evaluated so as to obtain differential partitioning of contaminant proteins and lactoperoxidase in top and bottom phases, respectively. PEG 6000-potassium phosphate system was found to be suitable for the maximum activity recovery of lactoperoxidase 150.70% leading to 2.31-fold purity. Further, concentration and purification of enzyme was attempted using ultrafiltration. The activity recovery and purification factor achieved after ultrafiltration were 149.85% and 3.53-fold, respectively. To optimise productivity and cost-effectiveness of integrated process, influence of ultrasound for the enhancement of permeate flux during ultrafiltration was also investigated. Intermittent use of ultrasound along with stirring (2 min acoustic and 2 min stirring) resulted in increased permeate flux from 0.94 to 2.18 l/m(2) h in comparison to the ultrafiltration without ultrasound. The use of ultrasound during ultrafiltration resulted in increase in flux, but there was no significant change in activity recovery and purification factor. The integrated approach involving ATPE and ultrafiltration may prove to be a feasible method for the downstream processing of lactoperoxidase from milk whey.

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confidence: 99%

Integrated Downstream Processing of Lactoperoxidase from Milk Whey Involving Aqueous Two-Phase Extraction and Ultrasound-Assisted Ultrafiltration

Nandini

Rastogi

2010

Appl Biochem Biotechnol

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“…A large-scale SMB process with five-zones was reported for six-sugar separation from biomass hydrolyzate (Xie et al 2005). A production-scale SMB process with five zones of 20 columns was presented for valuable protein separation from whey protein concentrate (Andersson and Mattiasson 2006). The Parex unit for p-xylene separation from its C 8 aromatic isomers (m-xylene, o-xylene, and ethylbenzene) was composed of a seven-zone SMB to increase the purity by flushing bed-lines contaminated by impurities (Minceva and Rodrigues 2003).…”

Section: Bmentioning

confidence: 99%

Effect of dead volume on performance of simulated moving bed process

Lim

Bhatia

2010

Adsorption

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The volume of surrounding equipments (pipe transfer lines and valves) in the simulated moving bed (SMB) unit, which is called the dead volume, is modeled as bed-head, bed-tail and bed-line. Since the dead volume can be significant especially in industrial-scale SMB units, the consideration of dead volume has been required for high performance operation. In this study, a simple and unified approach based on the method of characteristics (MOC), called the extended node model, is established to solve fluid concentration dynamics within dead volumes. The computational efficiency of the approach is evaluated for three case studies of a standard four-zone SMB process with a linear adsorption equilibrium model. Insertion of one zone to flush the fluid trapped in extract bed-line into the standard fourzone SMB improves substantially purity, while recovery is kept constant.

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“…More elaborate separations have been designed including ternary [24] and gradient separations [25]. Nonetheless, the limitations of SMB are compensated by higher efficiency in terms of separation of binary mixtures at higher concentrations at which they would normally not be resolved, and achieving such difficult separations with lower solvent consumption than batch chromatography [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…2A and B). Hence, SMB chromatography enables enhanced throughput with lower solvent consumption over a shorter period of time compared to batch chromatography [19,20]. This chromatographic technique has been successfully used in separation of hydrocarbons and fine chemicals (particularly enantiomers) in scale-up operations that results in high productivity and low solvent consumption [21].…”

Section: Introductionmentioning

confidence: 99%

Simulated moving bed purification of flaxseed oil orbitides: Unprecedented separation of cyclolinopeptides C and E

Okinyo-Owiti

Burnett

Reaney

2014

Journal of Chromatography B

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Simulated moving bed technology with a simplified approach for protein purification

Cited by 77 publications

References 29 publications

Integrated Downstream Processing of Lactoperoxidase from Milk Whey Involving Aqueous Two-Phase Extraction and Ultrasound-Assisted Ultrafiltration

Integrated Downstream Processing of Lactoperoxidase from Milk Whey Involving Aqueous Two-Phase Extraction and Ultrasound-Assisted Ultrafiltration

Effect of dead volume on performance of simulated moving bed process

Simulated moving bed purification of flaxseed oil orbitides: Unprecedented separation of cyclolinopeptides C and E

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