Simulating Biodegradation of Hydrocarbon Pollutants under Slow Nutrient Delivery Conditions

Sampson, T; Ogugbue, Chimezie Jason; Okpokwasili, G. C.

doi:10.9734/bmrj/2016/25956

Cited by 1 publication

(3 citation statements)

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“…Some of the isolates Pseudomonas aeruginosa and Bacillus sp. have been reported in some articles to produce organic materials that possess biosurfactant or emulsification properties and thereby increasing the solubility and bioavailability of components for microbial activities (Effiong et al, 2019;Sampson et al, 2016). This study agrees strongly with the report of Udume et al (2019) who reported the preponderance of Bacillus spp and Pseudomonas sp from their separate investigations, especially spore formers.…”

Section: Resultssupporting

confidence: 91%

“…It was also in a previous study conducted by Stanley et al (2017) using combinations of agro-residues and spawn of Pleurotus sp. The work of Sampson et al (2016) suggests that microbes converting harmful pollutants into less harmful ones follow a predictable and model-feasible bioprocess. According to Osuji and Onojake (2004), the bioavailability of heavy metals in polluted soil is controlled by the absorption coefficients and geophysical factors.…”

Section: Resultsmentioning

confidence: 99%

“…The bacterial inoculum was prepared as described by Sampson et al, (2016). In this method, pure cultures of Bacillus sp and Pseudomonas sp was suspended in nutrient broth and incubated at 37 0 C for 24 hours.…”

Section: Preparation Of Bacterial Inoculummentioning

confidence: 99%

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Bioremediation of heavy metals from artisanal crude oil refinery (Kpo-Fire) impacted soil using Bacillus flexus and Pseudomonas aeruginosa in Ngie Community, Degema Local Government Area, Rivers State, Nigeria

Ugboma¹,

Sampson²,

Mbonu³

2021

jasem

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The existence of heavy metals in “kpo-fire” impacted soil creates significant risks to human health and the ecosystem. In this study, the efficacy of the elimination of heavy metal from “kpo-fire” impacted soil was evaluated using bacterial treatments. The organisms (Bacillus flexus and Pseudomonas aeruginosa) used in the bioremediation setup were isolated from the impacted soil. Heavy metal analysis was carried out using an Atomic Absorption Spectrophotometer. The experimental setup involved the recreation of the contaminated soil sample in three (3) vessels labeled as: Flask A containing 300g of un-amended sample (control) to monitor natural process; Flask B containing 300g of sterilized sample; Flask C containing 300g of sample with Pseudomonas aeruginosa and Bacillus flexus. Soil baseline physicochemical composition was determined to have a pH of 6.18, Temperature of 29.2oC, Total Organic Carbon 7.58 mg/kg and Phosphate concentration 37.56 mg/kg. At the end of the investigation, experimental setup C, containing bacterial inocula was observed to possess the best bio-removal rates for Mercury (99.32%), Cadmium (77.59%), Boron (72.84%) and Arsenic (93.43%) after a 42-day period of study. Also, the concentrations declined from 1.05264mg/kg to 0.00621mg/kg for Mercury; Cadmium declined from 5.93mg/kg to 1.16mg/kg; Boron declined from 3.61mg/kg to 0.82mmg/kg and Arsenic declined from 2.78mg/kg to 0.16mg/kg. Molecular characterization revealed the contaminated soil had predominance of isolates with genomic molecular weight of 1,500 bp and the phylogenetic construct showed the bacterial isolates were Pseudomonas aeruginosa (MT023359), Bacillus flexus (MT023375) and Lysinibacillus macroides (MT023377). Statistical analysis revealed a strong positive correlation between the bacterial biomass and heavy metal removal. The synergistic parts played by bacterial consortia in the bio-removal of heavy metals from the polluted soil have been established and these potentials can be harnessed as a roadmap for eco-recovery of impacted environment in the Niger Delta. Bacillus flexus and Pseudomonas aeruginosa in consortium are efficient in remediation of kpo-fire contaminated soil.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%