Simulating STEM Imaging of Nanoparticles in Micrometers-Thick Substrates

Demers, Hendrix; Poirier-Demers, N; Drouin, Dominique; Jonge, Niels de

doi:10.1017/s1431927610094080

Cited by 42 publications

(46 citation statements)

References 31 publications

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“…This can be explained on the basis of the atomic number (Z) contrast of STEM Demers et al, 2010); TEM most frequently used for biological microscopy would require a much higher dose to obtain a similar resolution for the involved sample thickness.…”

Section: Principle Of Operationmentioning

confidence: 99%

Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells

Peckys

Jonge

2014

Microsc Microanal

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Scanning transmission electron microscopy (STEM) of specimens in liquid, so-called Liquid STEM, is capable of imaging the individual subunits of macromolecular complexes in whole eukaryotic cells in liquid. This paper discusses this new microscopy modality within the context of state-of-the-art microscopy of cells. The principle of operation and equations for the resolution are described. The obtained images are different from those acquired with standard transmission electron microscopy showing the cellular ultrastructure. Instead, contrast is obtained on specific labels. Images can be recorded in two ways, either via STEM at 200 keV electron beam energy using a microfluidic chamber enclosing the cells, or via environmental scanning electron microscopy at 30 keV of cells in a wet environment. The first series of experiments involved the epidermal growth factor receptor labeled with gold nanoparticles. The labels were imaged in whole fixed cells with nanometer resolution. Since the cells can be kept alive in the microfluidic chamber, it is also feasible to detect the labels in unfixed, live cells. The rapid sample preparation and imaging allows studies of multiple whole cells.

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Section: Principle Of Operationmentioning

confidence: 99%

Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells

Peckys

Jonge

2014

Microsc Microanal

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“…34,[202][203][204] This approach was used to enhance the spatial resolution in a fluidic cell for TEM where the liquid path length was micrometers thick, instead of the desired nanometer thickness. 34,[202][203][204] Electron energy is also an important consideration when developing optimized sensing schemes-whether it be the incident electron energy or the transmitted electron energy. For example, energy-filtering of the electrons just before detection can improve the spatial resolution of the image, albeit it at the cost of detected electron intensity.…”

Section: Nanofluidic Sample Cellsmentioning

confidence: 99%

Through a Window, Brightly: A Review of Selected Nanofabricated Thin-Film Platforms for Spectroscopy, Imaging, and Detection

Dwyer

Harb

2017

Appl Spectrosc

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We present a review of the use of selected nanofabricated thin films to deliver a host of capabilities and insights spanning bioanalytical and biophysical chemistry, materials science, and fundamental molecular-level research. We discuss approaches where thin films have been vital, enabling experimental studies using a variety of optical spectroscopies across the visible and infrared spectral range, electron microscopies, and related techniques such as electron energy loss spectroscopy, X-ray photoelectron spectroscopy, and single molecule sensing. We anchor this broad discussion by highlighting two particularly exciting exemplars: a thin-walled nanofluidic sample cell concept that has advanced the discovery horizons of ultrafast spectroscopy and of electron microscopy investigations of in-liquid samples; and a unique class of thin-film-based nanofluidic devices, designed around a nanopore, with expansive prospects for single molecule sensing. Free-standing, low-stress silicon nitride membranes are a canonical structural element for these applications, and we elucidate the fabrication and resulting features-including mechanical stability, optical properties, X-ray and electron scattering properties, and chemical natureof this material in this format. We also outline design and performance principles and include a discussion of underlying material preparations and properties suitable for understanding the use of alternative thin-film materials such as graphene.

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“…The spatial resolution achieved is remarkably high, and is due to the Z contrast of STEM Demers et al, 2010;Schuh & de Jonge, 2014). It would not be possible to achieve nanoscale resolution at these thicknesses with transmission electron microscopy.…”

Section: Model Of the Biotinylated Anti-her2 Affibody (Blue) Bindinmentioning

confidence: 99%

Membrane protein stoichiometry studied in intact mammalian cells

Jonge¹

2016

infocus

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Simulating STEM Imaging of Nanoparticles in Micrometers-Thick Substrates

Cited by 42 publications

References 31 publications

Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells

Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells

Through a Window, Brightly: A Review of Selected Nanofabricated Thin-Film Platforms for Spectroscopy, Imaging, and Detection

Membrane protein stoichiometry studied in intact mammalian cells

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