Recently, the surrogate safety assessment model has been considered for the safety performance analysis of roundabouts. This model can perform a detailed analysis of conflicts based on the trajectory data of vehicles inside the roundabout. The Separated Central Island (SCI) roundabout, as one of the roundabouts with distinct geometrical characteristics, holds the potential for safety evaluation using the conflict-based method. Therefore, in this study, a safety diagnosis of an SCI roundabout was conducted for the first time. In this study, a safety diagnosis procedure for SCI roundabout was first defined; for this purpose, vehicle trajectory data were obtained using an Unnamed Aerial Vehicle (UAV) and then entered into Surrogate Safety Measure Model software (SSAM 3.0). A case study was conducted on the only SCI roundabout in Iran, located in Mashhad. The parameters of Time-to-Collision (TTC), maximum speed difference of two vehicles involved in a collision (MaxΔV), and maximum speed of vehicles in collision (MaxS) were utilized to determine the type and severity of conflicts and risk analysis. The results of roundabout risk analysis showed that the severity level of conflicts is mainly of the injury type and that the lowest severity of conflicts is related to fatality. In addition, the highest frequency of injury conflicts is associated with lane-change conflict and the lowest frequency is linked to rear-end conflict. The highest and lowest frequency of damage conflicts are related to crossing and lane-change conflicts, respectively. After overall risk scoring, the severity level of conflicts is mainly related to injury type and the lowest severity of conflicts is associated with fatality; 31% of the total conflicts obtained are of the damaging type, and 69% are of the injury type. Finally, comparing the results of the conflict data with the 8-year crash data in such roundabouts confirms that in the absence of crash data in such roundabouts traffic engineers can use the roundabout analysis based on this study to predict the safety situation of such roundabouts before implementing engineering processes.