Simulation and optimisation of the downstream process for purification of human serum albumin by using ion exchange membrane adsorbers

Frerick, Constantin; Kreis, Peter; Górak, Andrzej; Melzner, Dieter

doi:10.1016/j.desal.2006.03.398

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…To cope with these limitations, the new method for ion exchange membrane chromatography (IEM) using ameliorated adsorbers was developed. The simulation studies which compare the IEM with IEC were done by Frerick, et al 69,70 Comparing to columnbased separations, the membrane chromatography has some advantages such as high separation efficiencies due to shorter diffusion times, [71][72][73] reduction in buffer usage, small floor space requirements, 74 simple method without complex hardware or packing necessities. 75 In spite of this advantages, expensive membrane and low efficiency of this technique for industrial purposes is an important drawback which causes the new technology | 501…”

Section: Ion Exchange Chromatography (Iec)mentioning

confidence: 99%

Overview of Albumin and Its Purification Methods

et al. 2016

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Section: Ion Exchange Chromatography (Iec)mentioning

confidence: 99%

Overview of Albumin and Its Purification Methods

et al. 2016

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Practical experiences from the development of aqueous two‐phase processes for the recovery of high value biological products

Benavides

Rito‐Palomares

2007

J of Chemical Tech & Biotech

142

122

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The development of recovery processes using aqueous two-phase systems (ATPS) has been limited by the extensive experimental work required to establish the optimal system parameters to ensure selective partitioning of the product of interest. Although using full factorial experiments has been demonstrated to be an effective strategy for the characterization of the partitioning behaviour of biological products in ATPS, this approach is characterized by its costly and time consuming nature. As an alternative, the use of a robotic-aided strategy has been proposed. However, the need for high cost equipment may limit the generic implementation of this strategy. Based on practical experience using ATPS, practical strategies for the predictive design of primary recovery processes using polymer-salt systems were derived. To evaluate the generic application of the proposed strategies, four experimental models (B-phycoerythrin, C-phycocyanin, double layered rotavirus-like particles and lutein) were selected. The application of these strategies resulted in the development of simplified recovery processes for the selected experimental models. The practical review presented is considered a relevant contribution that will provide general rules to facilitate the establishment of ATPS processes, particularly for new researchers in the field.

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Simulation and optimisation of the downstream process for purification of human serum albumin by using ion exchange membrane adsorbers

Cited by 2 publications

References 2 publications

Overview of Albumin and Its Purification Methods

Overview of Albumin and Its Purification Methods

Practical experiences from the development of aqueous two‐phase processes for the recovery of high value biological products

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