Simulation experiments of the effect of space environment on bacteriophage and DNA thin films

Fekete, Andrea; Rontó, Gy.; Hegedüs, M.; Módos, Károly; Bérces, Attila; Kovács, G.; Lämmer, H.; Panitz, Corinna

doi:10.1016/j.asr.2003.08.037

Cited by 16 publications

(5 citation statements)

References 20 publications

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“…Exposure to vacuum caused a similar effect to dehydration (0% relative humidity) [12,23]. Vacuum causes mostly dehydration, which leads to the loss of base stacking and this can be detected spectroscopically.…”

Section: Discussionmentioning

confidence: 91%

Response of bacteriophage T7 biological dosimeter to dehydration and extraterrestrial solar UV radiation

Hegedüs¹,

Fekete²,

Módos³

et al. 2007

Acta Astronautica

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Section: Discussionmentioning

confidence: 91%

Response of bacteriophage T7 biological dosimeter to dehydration and extraterrestrial solar UV radiation

Hegedüs¹,

Fekete²,

Módos³

et al. 2007

Acta Astronautica

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“…Under the influence of short-wavelength solar UV radiation (UVC) photo-products are induced, whereby reversion can be stimulated by photons with similar energies (Ronto´et al 1967;Fekete et al 2004). Irradiation experiments on uracil thin layers gave evidence of a higher monomerization efficiency of a deuterium lamp (short UVC) and that of a higher dimerization efficiency of a low-pressure Hg lamp (254 nm) as presented in Be´rces et al (2002).…”

Section: Uv Radiation and The Interaction With Organic And Biologicalmentioning

confidence: 84%

An ultraviolet simulator for the incident Martian surface radiation and its applications

Kolb

Abart

Bérces

et al. 2005

International Journal of Astrobiology

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: Ultraviolet (UV) radiation can act on putative organic/biological matter at the Martian surface in several ways. Only absorbed, but not transmitted or reflected, radiation energy can be photo-chemically effective. The most important biological UV effects are due to photochemical reactions in nucleic acids, DNA or RNA, which constitute the genetic material of all cellular organisms and viruses. Protein or lipid effects generally play a minor role, but they are also relevant in some cases. UV radiation can induce wavelengths-specific types of DNA damage. At the same time it can also induce the photo-reversion reaction of a UV induced DNA photoproduct of nucleic acid bases, the pyrimidine dimers. Intense UVB and UVC radiation, experienced on early Earth and present-day Mars, has been revealed to be harmful to all organisms, including extremophile bacteria and spores. Moreover, the formation of oxidants, catalytically produced in the Martian environment through UV irradiation, may be responsible for the destruction of organic matter on Mars. Following this, more laboratory simulations are vital in order to investigate and understand UV effects on organic matter in the case of Mars. We have designed a radiation apparatus that simulates the anticipated Martian UV surface spectrum between 200 and 400 nm (UVC-UVA). The system comprises a UV enhanced xenon arc lamp, special filter-sets and mirrors to simulate the effects of the Martian atmospheric column and dust loading. We describe the technical setup and performance of the system and discuss its uses for different applications. The design is focused on portability, therefore, the Mars-UV simulator represents a device for several different Mars simulation facilities with specific emphasis on Mars research topics.

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“…For wavelengths larger than approximately 290 nm, the optical density was nearly zero (< 0.005), and the optical density decreased with increasing wavelength within 200 to 290 nm. In a previous study (Fekete et al, 2004), the optical density for nucleic acids which are a kind of intracellular material (internal structure components) was nearly zero at wavelengths larger than 290 nm. Fig.…”

Section: Resultsmentioning

confidence: 99%

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Park

Yoon

Byeon³

et al. 2012

Aerosol Air Qual. Res.

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We evaluated a method for the assessment of bacterial viability that is based on ultraviolet and visible (UV-Vis) spectroscopy analysis. The quantities of intracellular materials inside a cell vary depending on change of bacterial viability by disruption of the membrane integrity. Therefore, normalized optical density in the range of 200-290 nm was analyzed to determine if it varied in samples containing different proportions of live bacteria. Our results indicate that samples containing higher proportions of live bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis had higher optical densities. In addition, the optical density at 230 nm divided by the optical density at 670 nm was found to have a strong linear correlation with bacterial viability (the R 2 values of E. coli, B. subtilis, and S. epidermidis are 0.9964, 0.9118, and 0.9861, respectively). Our proposed rapid assessment method takes less than three minutes and only requires optical measurements at 230 and 670 nm; therefore, it is simpler and faster than colony counting, fluorochromasia, or the dyeexclusion test. Moreover, our method was applied to bioaerosols, which are currently important issues in public health, microbiology, aerosol science, and other fields. In our study, the bacteria (E. coli) were dispersed into the air using a Collisontype atomizer, and were sampled in sterilized deionized water using an impinger with a pump. According to our method, the viability of E. coli was approximately 55.2%, which was similar to 52.5 ± 4.7% determined from the LIVE/DEAD BacLight bacterial viability assay.

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Simulation experiments of the effect of space environment on bacteriophage and DNA thin films

Cited by 16 publications

References 20 publications

Response of bacteriophage T7 biological dosimeter to dehydration and extraterrestrial solar UV radiation

Response of bacteriophage T7 biological dosimeter to dehydration and extraterrestrial solar UV radiation

An ultraviolet simulator for the incident Martian surface radiation and its applications

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

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