This review explores the synergistic relationship between advanced microscopy techniques and mechanical engineering, outlining their profound impact on materials science and mechanical system design. We delve into the multifaceted applications of electron microscopy, X-ray diffraction, and spectroscopic methods in understanding microstructural dynamics, mechanical properties, and failure mechanisms of materials integral to mechanical engineering. Through a comprehensive synthesis of recent research, we emphasize the pivotal role these techniques play in optimizing material performance, bolstering structural integrity, and driving innovation in mechanical design. By elucidating the intricate details of material behavior at the microscale, advanced microscopy contributes to informed decision-making in material selection and design processes. Furthermore, we address emerging trends and prospects, underscoring the continued synergy between advanced microscopy and mechanical engineering. This collaboration remains at the forefront of materials science and technology, promising ongoing advancements that will shape the future landscape of mechanical design and materials innovation.