Simulation of Cardiac Cell-Seeded Membranes Using the Edge-Based Smoothed FEM

Frotscher, Ralf; Goßmann, Matthias; Raatschen, Hans-Juergen; Artmann, A.; Staat, Manfred

doi:10.1007/978-3-319-02535-3_11

Cited by 4 publications

(12 citation statements)

References 25 publications

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“…Cells were delivered live in standard cell culture flasks. After trypsinization, they were seeded on CellDrum membranes at a density of 150,000 cells cm -² (a representative figure in [42] shows the irregular assembly of the cells in the confluent monolayer on a CellDrum membrane). The culture medium was replaced every day.…”

Section: Cell Culturingmentioning

confidence: 99%

Mechano-Pharmacological Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells

Goßmann

Frotscher

Linder³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.

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Section: Cell Culturingmentioning

confidence: 99%

Mechano-Pharmacological Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells

Goßmann

Frotscher

Linder³

et al. 2016

Cell Physiol Biochem

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“…Due to a missing cell type specific parametrization an equal parametrization was used for all cell types. For the whole tissue construct the cellular reference stress was determined to be D 50I = 0.5808 kPa [26] which replaced the original model parameter [10].…”

Section: Electromechanical Couplingmentioning

confidence: 99%

“…Global isotropy of the muscle fibers is given in the thin cardiac tissue construct on the CellDrum [26], thus…”

Section: Mechanical Modelmentioning

confidence: 99%

“…Clamped in a fixed ring the composite material can be inflated by a syringe pump and the resulting cellular contraction-dependent deflection is measured using a laser sensor. Thus, parameters for passive and coupling models can be determined [26]. Drugs have already been applied to the culture medium leading to phenomenologically observable changes in the beating frequency, deflection, contraction duration, activation time, relaxation time and resting time [25].…”

Section: Introductionmentioning

confidence: 99%

“…Drugs have already been applied to the culture medium leading to phenomenologically observable changes in the beating frequency, deflection, contraction duration, activation time, relaxation time and resting time [25]. Frotscher et al [14,26,27] developed an electromechanical model of the CellDrum which they parametrized, verified and validated using respective experiments. On the level of the electrophysiological cell models also the drug action was implemented.…”

Section: Introductionmentioning

confidence: 99%

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A 3d Electromechanical Fem-Based Model for Cardiac Tissue

Dương¹,

Jung²,

Frotscher³

et al. 2016

Proceedings of the VII European Congress on Computational Methods in Applied Sciences and Engineering (ECCOMAS Congress 2016)

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Modelling of Soft Connective Tissues to Investigate Female Pelvic Floor Dysfunctions

Bhattarai

Staat

2018

Computational and Mathematical Methods in Medicine

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After menopause, decreased levels of estrogen and progesterone remodel the collagen of the soft tissues thereby reducing their stiffness. Stress urinary incontinence is associated with involuntary urine leakage due to pathological movement of the pelvic organs resulting from lax suspension system, fasciae, and ligaments. This study compares the changes in the orientation and position of the female pelvic organs due to weakened fasciae, ligaments, and their combined laxity. A mixture theory weighted by respective volume fraction of elastin-collagen fibre compound (5%), adipose tissue (85%), and smooth muscle (5%) is adopted to characterize the mechanical behaviour of the fascia. The load carrying response (other than the functional response to the pelvic organs) of each fascia component, pelvic organs, muscles, and ligaments are assumed to be isotropic, hyperelastic, and incompressible. Finite element simulations are conducted during Valsalva manoeuvre with weakened tissues modelled by reduced tissue stiffness. A significant dislocation of the urethrovesical junction is observed due to weakness of the fascia (13.89 mm) compared to the ligaments (5.47 mm). The dynamics of the pelvic floor observed in this study during Valsalva manoeuvre is associated with urethral-bladder hypermobility, greater levator plate angulation, and positive Q-tip test which are observed in incontinent females.

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Simulation of Cardiac Cell-Seeded Membranes Using the Edge-Based Smoothed FEM

Cited by 4 publications

References 25 publications

Mechano-Pharmacological Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells

Mechano-Pharmacological Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells

A 3d Electromechanical Fem-Based Model for Cardiac Tissue

Modelling of Soft Connective Tissues to Investigate Female Pelvic Floor Dysfunctions

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