Roller drafting, a key mechanical operation for attenuating slivers to a desired thickness, holds critical significance in the spinning processes of the textile industry. Within the drafting stage of spinning, the motion state of fibers is influenced by their intrinsic characteristics, the force applied, and the distribution of both fast-moving and slow-moving fibers. This research focused on man-made fibers, which are isometric, and studied their distributions in the roller drafting zone. In addition, the cut-off weighting method was incorporated to measure the fiber distributions in the drafting zone, the impact of drafting parameters on fiber distribution was discussed, and the associated fiber distribution curves were generated. Findings reveal that fiber distributions are highly affected by factors such as the fiber length, roller-setting, and draft ratio. Conversely, the input sliver linear density and doubling number exhibited negligible influence on the fiber distribution. The total fiber distribution curve appeared as a dichotomy, including a horizontal line and an oblique line. This study also presented a second-order regression model and a deduction model to predict the distribution of isometric fibers in the roller drafting zone.