Simultaneous analysis of 1176 gene products in normal human aorta and abdominal aortic aneurysms using a membrane-based complementary DNA expression array

Tung, William S.; Lee, Jason K.; Thompson, Robert W.

doi:10.1067/mva.2001.113310

Cited by 94 publications

(80 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7) Armstrong, et al compared AAA tissue with that from aortoiliac occlusive disease and normal aortas for 265 genes, and found aneurysm-specific decreased expression of a subunit of collagen. 8) Likewise, Absi, et al reported distinct patterns of gene expression in TAA and AAA tissues by comparing 1185 genes with normal aorta specimens.…”

Section: Discussionmentioning

confidence: 99%

“…Cathepsin D, the increased expression of which in atherosclerosis and aneurysm tissue has been well described, is a cysteine protease. 7,20,21) Apoptosis and inflammation: It is known that apoptosis of vascular smooth muscle cells is increased within the medial layer of aneurysmal aortic tissue. 13) In our microarray assays, apoptosis related genes such as caspase 4 and TNF family genes were upregulated.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6] Further, since the development of cDNA microarray methods, several studies have been performed to compare gene expression profiles of aneurysm specimens with those of normal aorta specimens, demonstrating many genes in aortic aneurysms were altered. [7][8][9] However, most of these reports compared aortic aneurysms with normal aorta obtained from non-age-matched cadavers, organ-transplant donors, or patients with aortoiliac occlusive diseases. Therefore, it remains unclear whether the differences found in gene expression were relevant to the aneurysms themselves, or were because of other factors such as age, atherosclerosis, hemodynamics, method or timing of tissue sampling, individual differences, or other systemic factors.…”

Section: Taketani Et Almentioning

confidence: 99%

See 2 more Smart Citations

Altered Patterns of Gene Expression Specific to Thoracic Aortic Aneurysms: Microarray Analysis of Surgically Resected Specimens

et al. 2005

View full text Add to dashboard Cite

SUMMARYChanges in the expression levels of several genes have been described in aortic aneurysm specimens, however, the spectrum of diverse molecular alterations remains to be elucidated. We attempted to identify key molecules that modulate the pathogenesis of aortic aneurysm, using a complimentary DNA microarray carrying approximately 13,000 human genes.Segments of thoracic aortic aneurysms (TAA) and adjacent normal thoracic aortic tissues without aneurysmal changes (NTA) were obtained from 20 patients undergoing graft surgery. RNA obtained from five pairs of TAA and NTA samples was compared to determine aneurysm-specific alterations using microarray. Further, the expression levels of several genes of interest were verified in the remaining specimens by real-time reverse transcription-polymerase chain reaction (RT-PCR).In microarray assays, several types of the matrix metalloproteinases were upregulated as reported previously. Also, 220 genes suggested to be involved in protein degradation, inflammation, apoptosis, stress response, intracellular signaling, and other processes were significantly upregulated. Many of these genes have not been previously implicated in cardiovascular disease. The real time RT-PCR independently confirmed that the expression levels of MMP-2, MMP-9, ADAMTS-1, and caspase 4 were consistently increased in TAA.The results indicate that many genes are involved in a complicated manner in the pathogenesis of TAA. Investigation of these genes will help clarify the pathogenesis of this disease, and may lead to the discovery of novel therapeutic targets. (Int Heart J 2005; 46: 265-277)

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Taketani Et Almentioning

confidence: 99%

See 1 more Smart Citation

Altered Patterns of Gene Expression Specific to Thoracic Aortic Aneurysms: Microarray Analysis of Surgically Resected Specimens

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Armstrong et al [45] showed no differences in MMP or TIMP expression between AAA and AOD specimens, while MMP-9 expression was increased in AOD and AAA specimens, compared to normal arteries. Other studies revealed increased expression of MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 in AAA compared to AOD [46][47][48][49].…”

Section: S Heeneman Et Almentioning

confidence: 99%

The dynamic extracellular matrix: intervention strategies during heart failure and atherosclerosis

Heeneman

Cleutjens

Faber

et al. 2003

The Journal of Pathology

120

106

View full text Add to dashboard Cite

The extracellular matrix is no longer seen as the static embedding in which cells reside; it has been shown to be involved in cell proliferation, migration and cell-cell interactions. Turnover of the different extracellular matrix components is an active process with multiple levels of regulation. Collagen, a major extracellular matrix constituent of the myocardium and the arterial vascular wall, is synthesized by (myo)fibroblasts in the myocardium and smooth muscle cells in the medial arterial vascular wall. Its degradation is controlled by proteinases, which include matrix metalloproteinases. This review will focus on the impact of fibrosis and especially collagen turnover on the progression of heart failure and atherosclerosis, two of the main cardiovascular pathologies. We will discuss data from human studies and animal models, with an emphasis on the effects of interventions on collagen synthesis and degradation. We conclude that there is a dynamic (dis)balance in the rate of collagen synthesis and degradation during heart failure and atherosclerosis, which makes the outcome of interventions not always predictable. Alternative approaches for intervening in collagen metabolism will be discussed as possible therapeutic intervention strategies.

show abstract

“…2 Irrespective of the type, it is well known that activation of the renineangiotensin (Ang) system, with a consequent increase in the production of Ang II, promotes the development of aneurysms. 1 It has been established that the expression of the Apoe gene is altered in patients with AAA, 3 and that Apoe À/À mice receiving exogenous Ang II develop AAA, and are therefore used as a model for human AAA. 4 AAA pathogenesis involves a series of events characterized by increased oxidative stress; inflammation caused by infiltration of immune cells resulting in production of cytokines/chemokines; apoptosis of smooth muscle cells; and activation of matrix metalloproteinase (MMP), resulting in degradation of elastic fibers and dilation of the aortic wall.…”

mentioning

confidence: 99%

Cytochrome P450 1B1 Contributes to the Development of Angiotensin II–Induced Aortic Aneurysm in Male Apoe−/− Mice

Thirunavukkarasu

Khan

Song

et al. 2016

The American Journal of Pathology

View full text Add to dashboard Cite

Cytochrome P450 (CYP) 1B1 is implicated in vascular smooth muscle cell migration, proliferation, and hypertension. We assessed the contribution of CYP1B1 to angiotensin (Ang) IIeinduced abdominal aortic aneurysm (AAA). Male Apoe À/À /Cyp1b1 þ/þ and Apoe À/À /Cyp1b1 À/À mice were infused with Ang II or its vehicle for 4 weeks; another group of Apoe À/À /Cyp1b1 þ/þ mice was coadministered the CYP1B1 inhibitor 2,3 0 ,4,5 0 -tetramethoxystilbene (TMS) every third day for 4 weeks. On day 28 of Ang II infusion, AAAs were analyzed by ultrasound and ex vivo by Vernier calipers, mice were euthanized, and tissues were harvested. Ang II produced AAAs in Apoe À/À /Cyp1b1 þ/þ mice; mice treated with TMS or Apoe À/À /Cyp1b1 À/À mice had reduced AAAs. Ang II enhanced infiltration of macrophages, T cells, and platelets and increased plateletderived growth factor D, Pdgfrb, Itga2, and matrix metalloproteinases 2 and 9 expression in aortic lesions; these changes were inhibited in mice treated with TMS and in Apoe À/À /Cyp1b1 À/À mice. Oxidative stress resulted in cyclooxygenase-2 expression in aortic lesions. These effects were minimized in Apoe À/À / Cyp1b1 þ/þ mice treated with TMS and in Apoe À/À /Cyp1b1 À/À mice and by concurrent treatment with the superoxide scavenger 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl. CYP1B1 contributed to the development of Ang IIeinduced AAA and associated pathogenic events in mice, likely by enhancing oxidative stress and associated signaling events. Thus, CYP1B1 may serve as a target for therapeutic agents for AAA in males. (Am J Pathol 2016 http://dx

show abstract

Simultaneous analysis of 1176 gene products in normal human aorta and abdominal aortic aneurysms using a membrane-based complementary DNA expression array

Cited by 94 publications

References 44 publications

Altered Patterns of Gene Expression Specific to Thoracic Aortic Aneurysms: Microarray Analysis of Surgically Resected Specimens

Altered Patterns of Gene Expression Specific to Thoracic Aortic Aneurysms: Microarray Analysis of Surgically Resected Specimens

The dynamic extracellular matrix: intervention strategies during heart failure and atherosclerosis

Cytochrome P450 1B1 Contributes to the Development of Angiotensin II–Induced Aortic Aneurysm in Male Apoe−/− Mice

Contact Info

Product

Resources

About