Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS)

Abstract

IntroductionIn recent years, trace elements have gained importance as biomarkers in many chronic diseases. Unfortunately, the requirement for sample volume increases with the extent of investigation either for diagnosis or elucidating the mechanism of the disease. Here, we describe the method development and validation for simultaneous determination of 25 trace elements (lithium [Li], beryllium [Be], magnesium [Mg], aluminium [Al], vanadium [V], chromium [Cr], manganese [Mn], iron [Fe], cobalt [Co], nickel [Ni], copper [Cu], zinc [Zn], gallium [Ga], arsenic [As], selenium [Se], rubidium [Rb], strontium [Sr], silver [Ag], cadmium [Cd], caesium [Cs], barium [Ba], mercury [Hg], thallium [Tl], lead [Pb], uranium [U]) using only 20 μL of human serum.MethodsSerum samples were digested with nitric acid and hydrochloric acid (ratio 1:1, v/v) and analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Seronorm®, a human-derived serum control material was used as quality control samples.ResultsThe coefficient of variations for both intra- and inter-day precisions were consistently <15% for all elements. The validated method was later tested on 30 human serum samples to evaluate its applicability.ConclusionWe have successfully developed and validated a precise and accurate analytical method for determining 25 trace elements requiring very low volume of human serum.

Association between bone trace elements and osteoporosis in older adults: a cross-sectional study

“…As we know, the accurate determination of trace elements in bone tissue mainly includes flame atomic absorption spectrometry (FAAS), inductively coupled plasma–mass spectrometry (ICP-MS), and EDX. 15–17 The bone tissue measured by the first two methods is incomplete because atomic absorption spectrometry (AAS) and ICP-MS require the removal of collagen, fat, bone marrow, and other components of bone samples through combustion or solvent dissolution, which may cause the loss of some trace elements. EDX is characterized by nondestructive sample analysis with the minimum amount of sample preparation, and samples can be detected under atmospheric pressure or in a low vacuum environment by polishing the sample surface.…”

Evaluation of Lead Ion in the Wastewater of the Lifting and Treatment Stations Using ICP-MS and CPE Methods

“…The most often used and recommended techniques are ICP-MS and inductively coupled plasma-optoelectronic spectroscopy (ICP-OES) because of their well-known advantages of sensitivity, selectivity, and multi-element analytical capabilities [27][28][29]. ICP-MS technology currently allows for the simultaneous identification of several trace elements [30]. In tests ranging from 1 ng/L to high mg/L ranges, ICP-MS was shown to be more accurate than other methods at detecting components of biological or clinical origin at the lowest concentrations with good linearity and exceptional sensitivity [31].…”

“…The metal ion in CPE should form a stable complex that is separable from the surfactant layer [38]. With a variety of KI solution concentrations (5,10,15,20,25,30,35, and 40 mmol/L) and a volume of 1 mL of KI solution, the effect of iodide concentration on the recovery value 6(a) illustrates that as more KI reagent was added the recovery value increased. The reagent quantity that produced the best results was 1 mL (30 mmol/L) of KI reagent [39].…”

Baseline Nickel Level in Saliva and Urine Among Healthy Malay Population

“…Moreover, with similar analytical technique and sample used, the documented baseline nickel level in saliva were heterogenous (Amini et al, 2012;Butt et al, 2020). Other studies which used blood serum to analyse the baseline nickel level conducted their experiment using different method and reported different values (Mohammed et al, 2020;Satija et al, 2014). Furthermore, published reports indicated that even with the same analytical technique used, a standardised level of nickel baseline has not been confirmed.…”

“…Furthermore, published reports indicated that even with the same analytical technique used, a standardised level of nickel baseline has not been confirmed. Most other studies used Inductively Coupled Plasma Mass Spectrometry (ICP-MS) due to its lowest detection limit and highly sensitive analytical technique for the determination of trace elements of clinical interest such as nickel from biological samples (Nisse et al, 2017;Mohammed et al, 2020;Komarova et al, 2021). Different types of biological samples were used in the past such as blood and hair.…”