Simultaneous analysis of amino acids and amines as their o-phthalaldehyde-ethanethiol-9-fluorenylmethyl chloroformate derivatives in cheese by high-performance liquid chromatography

Kőrös, Á.; Varga, Zsolt; Molnár-Perl, I.

doi:10.1016/j.chroma.2008.07.035

Cited by 96 publications

(32 citation statements)

References 44 publications

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“…Certainly these two derivatization protocols did have and they still have their uncertainties, limitations, shortages and advantages: however, in the last decade the overwhelming part of uncertainties were examined and cleared up: resulting in the trouble-free, conscious and reliable use the OPA [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and that of the FMOC [20,21] techniques, as well.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Advancement in the derivatizations of the amino groups with the o-phthaldehyde-thiol and with the 9-fluorenylmethyloxycarbonyl chloride reagents

Molnár-Perl

2011

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

“…; () = in parentheses percentages, expressed in the number of the total, given in the last two horizontal line. own experiences [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] the following questions had to be answered:…”

Section: Introductionmentioning

confidence: 99%

Advancement in the derivatizations of the amino groups with the o-phthaldehyde-thiol and with the 9-fluorenylmethyloxycarbonyl chloride reagents

Molnár-Perl

2011

Journal of Chromatography B

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“…They determined that MeOH-HCl resulted in a more complete recovery of cadaverine, putrescine, and histamine. Kőrös, Varga and Molnár-Perl (2008) evaluated the extraction step in cheese with four acids, one by one: perchloric, chloridic, sulfosalicylic and trichloroacetic and confirmed that perchloric acid provided a 30% higher yield, compared to the other acids.…”

Section: Sample Preparationmentioning

confidence: 90%

Métodos cromatográficos para determinar aminas biogênicas em alimentos de origem animal

Torre

Conté-Júnior

2013

Braz. J. Vet. Res. Anim. Sci.

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Biogenic amines (BAs) are formed as a result of specific free amino acid decarboxylation. Analysis of these metabolites may be of great importance to determine food quality and for monitoring the levels of biogenic amines such as histamine and tyramine related to intoxication episodes in humans. Chromatography is a chemistry separation technique used to characterize biogenic amines in foods. Variations of this technique (liquid, thin layer and gas chromatography) have been widely applied; however, the food matrix complex requires that changes in the methodology of extraction, derivatization and detection must be performed according to each group of foods. High-performance liquid chromatography is the most widely used chromatographic method applied for biogenic amines in foods. However, due to the current importance of biogenic amines in quality control and consumer safety, researchers try to develop new methods for a fast, reliable analysis of foods in the market. This review presents some chromatographic techniques applied to monitoring BAs in different foods of animal origin.Keywords: Biogenic amines. Chromatography. Foods of animal origin. ResumoAminas biogênicas são formadas como resultado da descarboxilação de aminoácidos livres específicos. A análise desses metabólitos é de grande importância na determinação da qualidade e monitoramento de biogênicas como histamina e tiramina relacionadas com episódios de intoxicação em humanos. A cromatografia é uma técnica de separação química usada para caracterizar aminas biogênicas. Variações da técnica (cromatografia líquida, em camada delgada e gasosa) têm sido amplamente usadas, porém a complexidade da matriz alimentar faz com que sejam realizadas mudanças nos processos de extração, derivatização e detecção em concordância com cada grupo de alimento. A cromatografia líquida de alta eficiência (CLAE) é o método mais utilizado na determinação de aminas biogênicas em alimentos. Contudo, devido à importância das aminas biogênicas no controle da qualidade e a segurança do consumidor, os pesquisadores tentam desenvolver novos métodos com o intuito de uma análise mais rápida e precisa para o controle de alimentos no mercado. O objetivo da revisão é apresentar algumas técnicas cromatográficas aplicadas no monitoramento de aminas biogênicas em produtos de origem animal.Palavras-chave: Alimentos de origem animal. Aminas biogênicas. Cromatografia.

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“…Sample broths were centrifuged at 10,000g for 10 Min, the supernatants were diluted 20 times with trichloroacetic acid and went through 0.22 μm filters. The supernatants were subsequently treated with o-phthalaldehyde reagent solution (Agilent Technologies) and then detected at 338 nm of emission wavelengths at a flow rate of 1 mL/Min according to the published method [25]. All reagent used were of chromatographic grade.…”

Section: Figmentioning

confidence: 99%

Enhancing pentose phosphate pathway in Corynebacterium glutamicum to improve l‐isoleucine production

Wang

et al. 2016

Biotech and App Biochem

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Three genes, gnd, pgl, and fbp, relevant to the pentose phosphate pathway (PPP) were overexpressed in Corynebacterium glutamicum IWJ001, leading to increase of l-isoleucine production. The transcriptional levels of gnd, pgl, and fbp significantly increased in IWJ001/pDXW-8-gnd-fbp-pgl. Compared with the control strain IWJ001/pDXW-8, intracellular NADPH/NADP ratios in IWJ001/pDXW-8-gnd and IWJ001/pDXW-8-gnd-fbp cells grown for 36 H increased threefold and fourfold, respectively, indicating that overexpression of gnd and fbp redirected the carbon flux to PPP. Intracellular NADPH/NADP ratio in IWJ001/pDXW-8-gnd-fbp-pgl grown for 36 H was similar to IWJ001/pDXW-8, suggesting that the NADPH produced by PPP could be quickly consumed for l-isoleucine production. 10.9 and 28.96 g/L of l-isoleucine was produced in IWJ001/pDXW-8-gnd-fbp-pgl in shake flask cultivation and fed-batch fermentation, respectively. In addition, IWJ001/pDXW-8-gnd-fbp-pgl grew fast, its dry cell weight reached 49 g/L after 48 H, whereas the start strain IWJ001/pDXW-8 reached only 40 g/L. After 96 H fermentation, l-isoleucine yield on glucose in IWJ001/pDXW-8-gnd-fbp-pgl reached 0.138 g/g. The results demonstrate that carbon flux redirection to PPP is an efficient approach to enhance l-isoleucine production in C. glutamicum.

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Simultaneous analysis of amino acids and amines as their o-phthalaldehyde-ethanethiol-9-fluorenylmethyl chloroformate derivatives in cheese by high-performance liquid chromatography

Cited by 96 publications

References 44 publications

Advancement in the derivatizations of the amino groups with the o-phthaldehyde-thiol and with the 9-fluorenylmethyloxycarbonyl chloride reagents

Advancement in the derivatizations of the amino groups with the o-phthaldehyde-thiol and with the 9-fluorenylmethyloxycarbonyl chloride reagents

Métodos cromatográficos para determinar aminas biogênicas em alimentos de origem animal

Enhancing pentose phosphate pathway in Corynebacterium glutamicum to improve l‐isoleucine production

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