Simultaneous analysis of cellular glycoproteome and phosphoproteome in cervical carcinoma by one-pot specific enrichment

Zheng, Haoyang; Sun, Nianrong; Deng, Chunhui

doi:10.1016/j.aca.2021.338693

Cited by 18 publications

(14 citation statements)

References 42 publications

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“…Compared with recent reports of offline enrichment, 16,25,32–37 the performance of our online analytical platform technology was competitive in different aspects (Table S1†). In addition, the quantitative reproducibility of this method was better with a correlation coefficient R 2 of 0.95 compared to the R 2 of 0.89 obtained for offline enrichment in a recent work, 38 and was even comparable to its offline data-independent acquisition (DIA) method ( R 2 = 0.93).…”

Section: Resultsmentioning

confidence: 78%

An eco-friendly, low-cost, and automated strategy for phosphoproteome profiling

et al. 2022

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Section: Resultsmentioning

confidence: 78%

An eco-friendly, low-cost, and automated strategy for phosphoproteome profiling

et al. 2022

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“…and TiO 2 (FZT-GSH), as shown in Figure 3b, had been applied for simultaneous purification of glycopeptides and phosphopeptides from complex samples [128]. GSH modification improved the hydrophilicity of the material, made it more evenly dispersed in the buffer, and…”

Section: Magnetic Mos Materialsmentioning

confidence: 99%

“…Reprinted with permission from Deng et al. [128]; (C) Schematic illustration of the construction of Fe 3 O 4 @nSiO 2 @mSiO 2 @TiO 2 . Reprinted with permission from Yan et al.…”

Section: Sample Preparation and Enrichment For Protein Phosphorylatio...mentioning

confidence: 99%

Advances in proteomics sample preparation and enrichment for phosphorylation and glycosylation analysis

et al. 2022

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As the common and significant chemical modifications, post‐translational modifications (PTMs) play a key role in the functional proteome. Affected by the signal interference, low concentration, and insufficient ionization efficiency of impurities, the direct detection of PTMs by mass spectrometry (MS) still faces many challenges. Therefore, sample preparation and enrichment are an indispensable link before MS analysis of PTMs in proteomics. The rapid development of functionalized materials with diverse morphologies and compositions provides an avenue for sample preparation and enrichment for PTMs analysis. In this review, we summarize recent advances in the application of novel functionalized materials in sample preparation for phosphoproteomes and glycoproteomes analysis. In addition, this review specifically discusses the design and preparation of functionalized materials based on different enrichment mechanisms, and proposes research directions and potential challenges for proteomic PTMs research.

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“…Phosphorylation plays a crucial regulatory role in almost all cellular activities; hence, it is extensively being studied. − Abnormal phosphorylation processes may be the direct or indirect cause of more than 400 diseases, including cancer and diabetes. − Therefore, the study of protein phosphorylation is highly significant because it may provide vital information for humans to identify cancer markers and discover drug targets. − However, because of the low abundance of phosphorylated peptides obtained through protein digestion, numerous signals from nonphosphopeptides interfere with those of phosphopeptides . Thus, the development of an enrichment method with specificity for the analysis of protein phosphorylation is highly significant.…”

Section: Introductionmentioning

confidence: 99%

Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides

Zhang

Wang

Zhang

et al. 2022

ACS Biomater. Sci. Eng.

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Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment. However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography–mass spectrometry (LC–MS) arise. Moreover, the reusability of these materials to achieve sustainability was hardly investigated. To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol–gel method. This produced an organic–inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition. The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials. Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area. As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture. The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability. Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material. The results indicated that the material has good application prospects in the analysis of protein phosphorylation. Furthermore, TiO2-CM consists of green and cheap cellulose as the skeleton, and its synthesis process is environment-friendly, simple, and inexpensive.

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Simultaneous analysis of cellular glycoproteome and phosphoproteome in cervical carcinoma by one-pot specific enrichment

Cited by 18 publications

References 42 publications

An eco-friendly, low-cost, and automated strategy for phosphoproteome profiling

An eco-friendly, low-cost, and automated strategy for phosphoproteome profiling

Advances in proteomics sample preparation and enrichment for phosphorylation and glycosylation analysis

Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides

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