Simultaneous analysis of cytokinins, auxins and abscisic acid by combined immunoaffinity chromatography, high performance liquid chromatography and immunoassay

Fernández, Belén; Centeno, Marı́a Luz; Feito, Isabel; Tamés, Ricardo Sánchez; Rodrı́guez, Ana

doi:10.1002/pca.2800060108

Cited by 46 publications

(20 citation statements)

References 18 publications

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“…The frozen embryogenic calluses were lyophilised and powdered, prior to the extraction and purification of ABA-like compounds and several cytokinins (Cks): N 6 -isopentenyladenine (iP), zeatin riboside (t-ZR), dihydrozeatinriboside (DHZR), performed following Fernández et al (1995). Samples (100 mg dry weight) were extracted with 80% (v/v) methanol containing t-butylated hydroxytoluene by agitating at 4°C in the dark according to the method of Valdés et al (2002).…”

Section: Endogenous Hormone Analysismentioning

confidence: 99%

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

Park

Cho

Moon

et al. 2010

Plant Cell Tiss Organ Cult

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In an attempt to obtain insight into the differential responsiveness of different genotypes of Kalopanax septemlobus regarding their embryogenic capacity, several parameters such as endogenous hormonal levels, DNA content, embryogenic callus proliferation and somatic embryogenesis were studied in several genotypes of this plant. Also, to understand the effect of the age of the explants on their embryogenic capacity, the same parameters were studied in two embryogenic cell lines of different ages in the selected genotype. In the present study, it was observed that the cytokinins/abscisic acid (ABA) ratio plays an important role in embryogenic capacity in the studied genotypes of K. septemlobus species. A decrease in embryogenic capacity of callus was observed with increasing age, along with a marginal decrease in the DNA content of nuclei. Further, it can be suggested from our results that young embryogenic callus is a better choice for somatic embryo formation than the long-term-maintained callus in K. septemlobus.

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Section: Endogenous Hormone Analysismentioning

confidence: 99%

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

Park

Cho

Moon

et al. 2010

Plant Cell Tiss Organ Cult

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“…Extraction and subsequent purification of abscisic acid (ABA)-like compounds, and cytokinins were performed following the method of Fernandez et al (1995). Samples (100 mg dry weight) were extracted with 80% (v/v) methanol containing t-butylated hydroxytoluene by agitating at 4°C in the dark according to the method of Valdes et al (2003).…”

Section: Analysis Of Endogenous Phytohormonesmentioning

confidence: 99%

Micropropagation of Salix pseudolasiogyne from nodal explants

Park

Kim

Moon

et al. 2008

Plant Cell Tiss Organ Cult

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The effect of phytohormones on the breaking of dormancy of axillary buds in Salix pseudolasiogyne and their subsequent proliferation from nodal explants were examined. Nodal explants obtained from a 20-year-old S. pseudolasiogyne tree were cultured either on woody plant basal medium (WPM) or WPM supplemented with benzyladenine (BA, 2.2/4.4 lM), zeatin (1.1/2.2 lM), gibberillic acid (GA 3 , 2.9 and 14.5 lM), and GA 3 + BA (2.9 + 4.4 lM). Although axillary shoots developed in all the media, a higher percentage bud break occurred on BA supplemented media. To corroborate the results, endogenous levels of cytokinins [Cks, N 6 -isopentenyladenine (iP), zeatin riboside (t-ZR), dihydrozeatinriboside (DHZR)] and abscisic acid (ABA) were determined. On BA supplemented media, the levels of zeatin type (Z-type) of Cks were higher than those of isopentenyladenine type of Ck in the explants, while the ABA level was low. Axillary shoots did not grow well and became necrotic upon subculture to fresh basal WPM. In order to improve shoot growth, they were subcultured twice at a 4-week interval on to WPM supplemented with BA (2.2/4.4 lM), GA 3 (1.4 lM), or GA 3 + BA (1.4 + 4.4/2.9 + 4.4 lM). Maximal shoot growth (93%) was achieved on WPM supplemented with 2.2 lM BA. Comparative analyses of endogenous Cks revealed that higher Cks (Z-type Cks) were present in actively growing shoots. Rooting was readily achieved when the shoots were subcultured to WPM without phytohormones. The rooted plants were acclimatized well upon transplantation.

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“…Frozen pulp of grape samples was dissolved in 2.0 mL of phosphatebuffered saline (PBS) containing 0.1% (v/v) Tween-20 and 0.1% (w/v) gelatin (pH 7.5) to quantify free abscisic acid (ABA), jasmonic acid (JA), indole acetic acid (IAA) by ELISA following the protocol described by Fernández et al [21]. The mouse monoclonal antigen and antibodies against free ABA, JA and IAA were produced at phytohormones research institute, China Agricultural University, Beijing China.…”

Section: Determination Of Endogenous Hormones Contentmentioning

confidence: 99%

The Effect of Pre-Harvest Methyl Jasmonate Treatment on the Selected Volatile Compounds and Endogenous Hormones Contends in the Pulp of Grape Berries

Wu¹,

Rehman²,

Feng³

et al. 2017

J Plant Biochem Physiol

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Grapevine is one of the most valued and widely cultivated fruit crop worldwide, with their pleasant flavor and valuable health effects. During the consequent ripening at ambient temperature, the volatile compounds of table grape often decreased, to affect their sensory evaluation. The development of new and effective methods to increase the volatile compounds of berries is necessary. Present study was carried out to investigate the pre-harvest methyl jasmonate (MeJA) treatment on the selected volatile compounds and endogenous hormones content from 'Shine Muscat' berries pulp. The results indicated that, pre-harvest application of MeJA (0.1 mM or 0.01 mM) on grape berries generally enhanced the production of terpenes, like nerol, linalool, alpha-terpineol; While some C6 compounds were reduced, such as (E)-2-hexenol, hexanol, (Z)-3-hexenol, hexanal and (E)-2-hexenal. The endogenous hormones like IAA (indole acetic acid), ABA (abscisic acid) and JA (jasmonate acid) content were also changed after MeJA treatment. We also observed that MeJA palys a key role in fruit endogenous hormones level and volatile compounds by increasing the expression level of several related genes, such as aroma-related genes Vvter, Vv-syn and hormone-related genes VvOPR3, VvAuI, VvEth, VvNCED1. We hypothesize that, MeJA as an effective elicitor affects the volatile compounds by altering endogenous hormones level in berries pulp of 'Shine Muscat'.

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Simultaneous analysis of cytokinins, auxins and abscisic acid by combined immunoaffinity chromatography, high performance liquid chromatography and immunoassay

Cited by 46 publications

References 18 publications

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

Micropropagation of Salix pseudolasiogyne from nodal explants

The Effect of Pre-Harvest Methyl Jasmonate Treatment on the Selected Volatile Compounds and Endogenous Hormones Contends in the Pulp of Grape Berries

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