Simultaneous Analysis of Fatty Alcohols, Fatty Aldehydes, and Sterols in Thyroid Tissues by Electrospray Ionization-Ion Mobility-Mass Spectrometry Based on Charge Derivatization

Qi, Wei; Wang, Yunjun; Cao, Yuqi; Cao, Yanjing; Guan, Qing; Sun, Tuanqi; Zhang, Li; Guo, Yinlong

doi:10.1021/acs.analchem.0c01292

Cited by 38 publications

(22 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…其次这类衍生化反应将氨基等亲质子基团或带电基团取代偶联到被分析物分子链上, 提高被分析物的电离效率, 增强 MS 分析的灵敏度. 例如, Qi 等人 [138] 在同时分析脂肪醇、脂肪醛和甾醇时, 使用吡啶和亚硫酰氯对生物样品进行化学衍生化, 使得生物分子具有永久带电的标签, 显著提高 MS 检测的灵敏度. 最后一种功能：该类衍生化可促使亲水或者疏水基团偶联到分析物分子链上, 从而改变分析物亲疏水性, 增强被分析物的分离纯化效率, 降低 MS 检测中的离子抑制.…”

Section: F O R R E V I E W O N L Yunclassified

Chemical derivatization: recent advances in mass spectrometry imaging

Li¹,

Qin²,

Jiang³

et al. 2022

Sci. Sin.-Chim

View full text Add to dashboard Cite

Recently, mass spectrometry imaging (MSI) has been drawing more and more attention due to its continuous development and improvement as well as its vital role in the study of biological science. Many MSI-based strategies have been widely applied to the in situ qualitative and quantitative detection and imaging of endogenous/exogenous molecules in tissues. Among, matrix assisted laser desorption/ionization MSI (MALDI-MSI) and desorption electrospray ionization MSI (DESI-MSI) are generally regarded as two the most commonly used molecular imaging techniques. Although MSI, as a histology-based emerging molecular imaging technique, has significant advantages over other imaging techniques because it is a label-free technique which provides high sensitivity, high throughput, and molecular specificity derived from the use of mass spectrometers as detectors for a wide variety of ionized biomolecules in situ within a tissue section, it still has obvious shortcomings in the in situ analysis of low-polarity and neutral biomolecules. To solve this issue, the application of chemical derivatization technology in MSI is one of the most important approaches to overcome these deficiencies of MS molecular imaging. This review provides an overview of various chemical derivatization techniques, such as, esterification derivatization, acylation derivatization, addition reaction derivatization, substitution derivatization, oxidative derivatization, and other derivatizations, used in MS analysis, highlights the application of these chemical derivatization methods in the in situ detection and imaging of biomolecules in tissues by MALDI-MSI and DESI-MSI. It can be predicted that with the advance progress of chemical derivatization technology, the application scope of MSI will be further expanded in the fields of spatial metabolomics, spatial lipidomics, spatial proteomics, and spatial glycomics.

show abstract

Section: F O R R E V I E W O N L Yunclassified

Chemical derivatization: recent advances in mass spectrometry imaging

Li¹,

Qin²,

Jiang³

et al. 2022

Sci. Sin.-Chim

View full text Add to dashboard Cite

show abstract

“…Pyridine-d 5 labeled aliphatic aldehydes have been prepared as internal standards. Furthermore, d 0 /d 5 -pyridine could also be used to simultaneously label different functional groups including hydroxyls and carbonyls [141]. Similarly, aniline/ 13 C 6 -aniline could also label multiple functional groups to simultaneously quantify metabolites involved in central carbon and energy metabolism [104,106].…”

Section: Absolute Quantificationmentioning

confidence: 99%

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

2021

View full text Add to dashboard Cite

Carbonyl-containing metabolites widely exist in biological samples and have important physiological functions. Thus, accurate and sensitive quantitative analysis of carbonyl-containing metabolites is crucial to provide insight into metabolic pathways as well as disease mechanisms. Although reversed phase liquid chromatography electrospray ionization mass spectrometry (RPLC-ESI-MS) is widely used due to the powerful separation capability of RPLC and high specificity and sensitivity of MS, but it is often challenging to directly analyze carbonyl-containing metabolites using RPLC-ESI-MS due to the poor ionization efficiency of neutral carbonyl groups in ESI. Modification of carbonyl-containing metabolites by a chemical derivatization strategy can overcome the obstacle of sensitivity; however, it is insufficient to achieve accurate quantification due to instrument drift and matrix effects. The emergence of stable isotope-coded derivatization (ICD) provides a good solution to the problems encountered above. Thus, LC-MS methods that utilize ICD have been applied in metabolomics including quantitative targeted analysis and untargeted profiling analysis. In addition, ICD makes multiplex or multichannel submetabolome analysis possible, which not only reduces instrument running time but also avoids the variation of MS response. In this review, representative derivatization reagents and typical applications in absolute quantification and submetabolome profiling are discussed to highlight the superiority of the ICD strategy for detection of carbonyl-containing metabolites.

show abstract

“…In recent years, ultrahigh performance liquid chromatography (UPLC) coupled to ion mobility spectroscopy (IMS) and a quadrupole time-of-flight mass spectrometer (UPLC-IMS-QTof) has been shown to be a powerful technique for the untargeted screening of complex samples. − The ionized molecules pass through a drift cell under an electric field and collide with a buffer gas of neutral molecules (normally nitrogen or helium). The arrival time of the ionized molecules is related to their size, charge, and shape, and as such, IMS potentially allows the separation of isobaric and isomeric compounds that coelute in liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

Discovery and Characterization of Phenolic Compounds in Bearberry (Arctostaphylos uva-ursi) Leaves Using Liquid Chromatography–Ion Mobility–High-Resolution Mass Spectrometry

Song

Canellas

Dreolin

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

The characterization and quantification of phenolic compounds in bearberry leaves were performed using hyphenated ion mobility spectroscopy (IMS) and a quadrupole time-of-flight mass spectrometer. A higher identification confidence level was obtained by comparing the measured collision cross section ( TW CCS N2 ) with predicted values using a machine learning algorithm. A total of 88 compounds were identified, including 14 arbutin derivatives, 33 hydrolyzable tannins, 6 flavanols, 26 flavonols, 9 saccharide derivatives, and glycosidic compounds. Those most reliably reproduced in all samples were quantified against respective standards. Arbutin (47−107 mg/g), 1,2,3,4,6-pentagalloylglucose (6.6−12.9 mg/g), and quercetin 3-galactoside/quercetin 3glucoside (2.7−5.7 mg/g) were the most abundant phenolic components in the leaves. Quinic acid and ellagic acid were also detected at relatively high concentrations. The antioxidant activity of the most abundant compounds was evaluated. A critical view of the advantages and limitations of traveling wave IMS and CCS for the discovery of natural products is given.

show abstract

Simultaneous Analysis of Fatty Alcohols, Fatty Aldehydes, and Sterols in Thyroid Tissues by Electrospray Ionization-Ion Mobility-Mass Spectrometry Based on Charge Derivatization

Cited by 38 publications

References 33 publications

Chemical derivatization: recent advances in mass spectrometry imaging

Chemical derivatization: recent advances in mass spectrometry imaging

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

Discovery and Characterization of Phenolic Compounds in Bearberry (Arctostaphylos uva-ursi) Leaves Using Liquid Chromatography–Ion Mobility–High-Resolution Mass Spectrometry

Contact Info

Product

Resources

About