Simultaneous analysis of free amino acids and taurine-related compounds in deep-sea mussel tissues using reversed-phase HPLC

Nagasaki, Toshihiro; Koito, Tomoko; Nemoto, Suguru; Ushio, Hideki; Inoue, Koji

doi:10.1007/s12562-017-1143-8

Cited by 11 publications

(4 citation statements)

References 34 publications

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“…Hypotaurine, a substance similar to taurine, and hydrogen sulfide ion can be converted to thiotaurine, a nontoxic substance that can be passed to symbionts (Pruski and Fiala-Médioni 2003;Yancey 2005;Koito et al 2010;Nagasaki et al 2018;Kuroda et al 2021). As mussels are always exposed to sulfides in hydrothermal-vent water, gill cells must maintain high hypotaurine levels.…”

Section: Hypotaurine Of Deep-sea Musselsmentioning

confidence: 99%

“…In addition, GAT-1, a transporter for gamma-aminobutyric acid (GABA), may participate in this process (Kinjo et al 2019b). Moreover, cysteine dioxygenase (CDO) and cysteine sulfinate decarboxylase (CSAD) may synthesize hypotaurine from cysteine (Nagasaki et al 2015(Nagasaki et al , 2018.…”

Section: Hypotaurine Of Deep-sea Musselsmentioning

confidence: 99%

“…Therefore, these transporters and enzymes have not evolved specifically for adaptation to hydrothermal vents. Rather, they may have been co-opted for osmotic adaptation in an ancestor that inhabited shallow seas, although functions of individual components may have been modified to augment hypotaurine accumulation (Koito et al 2016;Nagasaki et al 2018). Interestingly, seawater ejected from hydrothermal vents at Myojin Knoll and Suiyo Seamount is slightly hypoosmotic relative to surrounding seawater (Fig.…”

Section: Hypotaurine Of Deep-sea Musselsmentioning

confidence: 99%

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Mussel biology: from the byssus to ecology and physiology, including microplastic ingestion and deep-sea adaptations

2021

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Mussels are a group of bivalves that includes the dominant species of shallow-sea, freshwater, and deep-sea chemosynthetic ecosystems. Mussels cling to various solid underwater surfaces using a proteinaceous thread, called the byssus, which is central to their ecology, physiology, and evolution. Mussels cluster using their byssi to form “mussel beds,” thereby increasing their biomass per unit of habitat area, and also creating habitats for other organisms. Clustered mussels actively filter feed to obtain nutrients, but also ingest pollutants and suspended particles; thus, mussels are good subjects for pollution analyses, especially for microplastic pollution. The byssus also facilitates invasiveness, allowing mussels to hitchhike on ships, and to utilize other man-made structures, including quay walls and power plant inlets, which are less attractive to native species. Physiologically, mussels have adapted to environmental stressors associated with a sessile lifestyle. Osmotic adaptation is especially important for life in intertidal zones, and taurine is a major component of that adaptation. Taurine accumulation systems have also been modified to adapt to sulfide-rich environments near deep-sea hydrothermal vents. The byssus may have also enabled access to vent environments, allowing mussels to attach to “evolutionary stepping stones” and also to vent chimneys.

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Section: Hypotaurine Of Deep-sea Musselsmentioning

confidence: 99%

Section: Hypotaurine Of Deep-sea Musselsmentioning

confidence: 99%

Section: Hypotaurine Of Deep-sea Musselsmentioning

confidence: 99%

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Mussel biology: from the byssus to ecology and physiology, including microplastic ingestion and deep-sea adaptations

2021

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“…The standards were also dried and derivatized as described above. The samples and standards were then filtered through 0.45-μm filters (Millipore, Billerica, Massachusetts, USA) and PITC-labeled taurine-related compounds and other amino acids were detected by reversed-phase high-performance liquid chromatography using a gradient program described by Nagasaki et al [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Difference in sulfur regulation mechanism between tube-dwelling and free-moving polychaetes sympatrically inhabiting deep-sea hydrothermal chimneys

Koito,

Ito,

Suzuki

et al. 2023

Zoological Lett

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The environment around deep sea hydrothermal vents is characterized by an abundance of sulfur compounds, including toxic hydrogen sulfide. However, numerous communities of various invertebrates are found in it. It is suggested that invertebrates in the vicinity of hydrothermal vents detoxify sulfur compounds by biosynthesis of taurine-related compounds in the body. On the other hand, the vent endemic polychaete Alvinella pompejana has spherocrystals composed of sulfur and other metals in its digestive tract. It was considered that the spherocrystals contribute to the regulation of sulfur in body fluids. Paralvinella spp. and Polynoidae. gen. sp. live sympatrically and in areas most affected by vent fluid. In this study, we focused on the digestive tract of Paralvinella spp. and Polynoidae. gen. sp. to examine whether they have spherocrystals. We also investigated the possible involvement of bacteria in the digestive tract in spherulization. Examination with a scanning electron microscope (SEM) equipped with Energy Disperse X-ray Spectroscopy (EDS) detected spherocrystals containing sulfur and iron in the digestive tract of Paralvinella spp. In contrast, such spherocrystals were not observed in that of Polynoidae. gen. sp. although sulfur is detected there by inductively coupled plasma-optical emission spectrometry (ICP-OES). Meta-16S rRNA analysis indicated that the floras of the digestive tracts of the two species were very similar, suggesting that enteric bacteria are not responsible for spherocrystal formation. Analysis of taurine-related compounds indicated that the digestive tissues of Polynoidae. gen. sp. contain a higher amount of hypotaurine and thiotaurine than those of Paralvinella spp. Therefore, the two sympatric polychaetes use different strategies for controlling sulfur, i.e., Paralvinella spp. forms spherocrystals containing elemental sulfur and iron in the digestive tract, but Polynoidae. gen. sp. accumulates taurine-related compounds instead of spherocrystals. Such differences may be related to differences in their lifestyles, i.e., burrow-dweller or free-moving, or may have been acquired phylogenetically in the evolutionary process.

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Taurine-related compounds and other free amino acids in deep-sea hydrothermal vent and non-vent invertebrates

et al. 2018

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Simultaneous analysis of free amino acids and taurine-related compounds in deep-sea mussel tissues using reversed-phase HPLC

Cited by 11 publications

References 34 publications

Mussel biology: from the byssus to ecology and physiology, including microplastic ingestion and deep-sea adaptations

Mussel biology: from the byssus to ecology and physiology, including microplastic ingestion and deep-sea adaptations

Difference in sulfur regulation mechanism between tube-dwelling and free-moving polychaetes sympatrically inhabiting deep-sea hydrothermal chimneys

Taurine-related compounds and other free amino acids in deep-sea hydrothermal vent and non-vent invertebrates

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