Simultaneous analysis of homovanillic acid, 5-hydroxyindoleacetic acid, 3-methoxy-4-hydroxy-phenylethylene glycol and vanilmandelic acid in plasma from alcoholics by high-performance liquid chromatography with electrochemical detection

Determination of 3,4-Dihydroxyphenylacetic Acid and 5-Hydroxyindoleacetic Acid in Human Plasma by a Simple and Rapid High-performance Liquid Chromatography Assay*

“…13 Another approach which has been used is liquid-liquid extraction after saturation of plasma samples with sodium chloride. 7,8 This technique has been shown to yield better recovery than alumina adsorption for some of the catecholamine metabolites and is not affected by the amount of sodium chloride. 8 However, these procedures are tedious and relatively labor intensive and would not solve the problems of interfering materials caused by medications or dietary constituents.…”

“…8,13 It offers sensitivity and specificity which are needed in biological analysis of these compounds, particularly in plasma. Using an electrochemical detector with the triple-electrode design as described, subpicrogram quantities of these compounds could be detected.…”

“…To each tube containing 10 mL of freshly collected blood was added 0.1 mL of a 0.1 M solution of sodium metabisulfite as antioxidant. 8 The tube was covered with Parafilm (American National Can, Greenwich, CT) and inverted several times to ensure mixing. The plasma was immediately separated by centrifugation (1760g, 4°C for 10 min) and stored at -70°C until analysis.…”

“…Different extraction techniques have been described to isolate catecholamines from plasma. [7][8][9][10][11][12] Most of these methods are laborious and time-consuming and will not resolve the problems of interference caused by medications. The present paper describes a simple, rapid and sensitive HPLC assay which would overcome the problems of interference caused by lipophilic substances (drugs and metabolites) for routine analysis of plasma 5-HIAA and DOPAC.…”

Determination of homovanillic acid (HVA) in human plasma by HPLC with coulometric detection and a new SPE procedure

“…Several analytical methods have been published for the determination of HVA in different biological matrices including urine [16,17], cerebro-spinal fluid [18], brain tissues [19], animal plasma [20,21] and other tissues [22]. Other methods regard the analysis of this compound in human plasma; the vast majority of them are based on the use of HPLC with electrochemical [15,21,[23][24][25][26][27][28][29] detection, however at least one uses HPLC with fluorescence detection [17] and another capillary gas chromatography-mass spectrometry [30].…”