Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay

Sergeev, N. V.; Volokhov, Dmitriy V.; Chizhikov, Vladimir; Rasooly, Avraham

doi:10.1128/jcm.42.5.2134-2143.2004

Cited by 91 publications

(41 citation statements)

References 40 publications

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“…It was also shown that all MRSA strains harbored SGF, SGFa and SGFb prophage types; therefore, the presence of sea, sek and seq genes was not surprising. This finding was not in agreement with those of other studies, in which the prevalence of these genes was significantly lower (12,15,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41). On the other hand, the high prevalence of sea, sek and seq genes was shown in MRSA strains isolated from patients in a referral hospital in Tehran, Iran (42).…”

Section: Discussioncontrasting

confidence: 55%

Isolation of Methicillin-Resistant Staphylococcus aureus Strains Producing Enterotoxins A, K and Q From Chicken Meat in Isfahan, Iran, 2014

Rahimi

Karimi

2016

Arch Clin Infect Dis

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Section: Discussioncontrasting

confidence: 55%

Isolation of Methicillin-Resistant Staphylococcus aureus Strains Producing Enterotoxins A, K and Q From Chicken Meat in Isfahan, Iran, 2014

Rahimi

Karimi

2016

Arch Clin Infect Dis

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“…Moreover, genes of the egc cluster were found in isolates recovered from nasal and blood specimens in high percentages (4,5). Recently, by applying the microarray technology for simultaneous detection of the SE genes, Sergeev et al (34) estimated that about 92% of S. aureus strains contain multiple SE genes (especially the egc cluster genes). Surveys of the egc distribution in S. aureus strains of animal origin also demonstrated a high frequency of egc-like genes (36).…”

Section: Resultsmentioning

confidence: 99%

Biotyping of Enterotoxigenic Staphylococcus aureus by Enterotoxin Gene Cluster ( egc ) Polymorphism and spa Typing Analyses

Blaiotta

Fusco

Eiff

et al. 2006

Appl Environ Microbiol

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Thirty-five Staphylococcus aureus strains, including 10 reference strains and 25 strains recovered from clinical specimens and food samples, were analyzed by PCR REA (restriction endonucleases analysis) of the egc operon and spa typing. Nineteen spa types and seven different egc operons, including four putative new egc variants, were revealed. In 13 strains, allelic variants of sei and/or seg were found. By an analysis of their nucleotide sequence identities, a new homogeneous cluster of a sei variant, called the sei variant, was detected in six strains. In addition, the prototype sei was shown to be more polymorphic than assumed so far. Seven strains possessed the recently described seg variant, also exhibiting several nucleotide exchanges. spa typing was more effective than REA egc grouping as a typing technique. Since, in some cases, the REA typing method was able to discriminate strains showing the same spa type, it must be considered for PCR approaches involved in diagnostic procedures and may be useful for epidemiological studies. Hence, the polyphasic approach used in this study can be reliably and advantageously applied for typing egc-positive S. aureus strains.Staphylococcus aureus is an extraordinarily versatile pathogen causing a wide spectrum of infections, ranging from mild to severe and life-threatening, in humans as well as economically important infections in animals. In addition to superficial lesions and systemic infections, S. aureus is responsible for toxin-mediated diseases, such as toxic shock syndrome (TSS) and staphylococcal food poisoning. The virulence factors causing these toxicoses are members of the family of bacterial pyrogenic toxin superantigens (PTSAgs) comprising the TSS-causing toxins and the staphylococcal enterotoxins (SEs) producing food-borne illness (11,24). Superantigens bypassing normal antigen presentation stimulate large populations of T cells by binding to a specific variable region of the T-cell antigen receptor beta chain (22). In addition to their nature as superantigens, SEs operate as potent gastrointestinal toxins, causing staphylococcal food poisoning, which has a major public health impact (18,28). Approximately 1.5 billion dollars are spent annually in the United States because of staphylococcal intoxications (38).Primarily, five major serological types, SEA through SEE, have been characterized (6). In the past years, many new types of SEs and their coding genes (seg through seu) have been reported (12, 16, 17, 19, 27, 30-33, 37, 37, 39, 40). However, some of the novel SE homologues were shown to be nonemetic, thus actually lacking the defining property of SEs and consequently designated "staphylococcal enterotoxin-like" superantigens (20). For SEC, minor variants have been reported (23). The staphylococcal PTSAgs constitute a large family of structurally related proteins whose genes are associated with mobile genetic elements. SEB, SEC, SEG, SEI, SEM, SEN, SEO, SEK, SEL, SEQ, and TSS toxin 1 are encoded by pathogenicity islands (2,16,17,21). SEA, SEE, and S...

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“…Microarray technologies have become a tool of choice for multiparametric diagnostic assays and for gene expression profiling (7,14,21,24). The ability to perform sensitive and reproducible microarray hybridizations depends to a great extent on the quality of the microarray substrate.…”

Section: Discussionmentioning

confidence: 99%

Plastic Polymers for Efficient DNA Microarray Hybridization: Application to Microbiological Diagnostics

et al. 2008

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Fabrication of microarray devices using traditional glass slides is not easily adaptable to integration into microfluidic systems. There is thus a need for the development of polymeric materials showing a high hybridization signal-to-background ratio, enabling sensitive detection of microbial pathogens. We have developed such plastic supports suitable for highly sensitive DNA microarray hybridizations. The proof of concept of this microarray technology was done through the detection of four human respiratory viruses that were amplified and labeled with a fluorescent dye via a sensitive reverse transcriptase PCR (RT-PCR) assay. The performance of the microarray hybridization with plastic supports made of PMMA [poly(methylmethacrylate)]-VSUVT or Zeonor 1060R was compared to that with high-quality glass slide microarrays by using both passive and microfluidic hybridization systems. Specific hybridization signal-to-background ratios comparable to that obtained with high-quality commercial glass slides were achieved with both polymeric substrates. Microarray hybridizations demonstrated an analytical sensitivity equivalent to approximately 100 viral genome copies per RT-PCR, which is at least 100-fold higher than the sensitivities of previously reported DNA hybridizations on plastic supports. Testing of these plastic polymers using a microfluidic microarray hybridization platform also showed results that were comparable to those with glass supports. In conclusion, PMMA-VSUVT and Zeonor 1060R are both suitable for highly sensitive microarray hybridizations.

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Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay

Cited by 91 publications

References 40 publications

Isolation of Methicillin-Resistant Staphylococcus aureus Strains Producing Enterotoxins A, K and Q From Chicken Meat in Isfahan, Iran, 2014

Isolation of Methicillin-Resistant Staphylococcus aureus Strains Producing Enterotoxins A, K and Q From Chicken Meat in Isfahan, Iran, 2014

Biotyping of Enterotoxigenic Staphylococcus aureus by Enterotoxin Gene Cluster ( egc ) Polymorphism and spa Typing Analyses

Plastic Polymers for Efficient DNA Microarray Hybridization: Application to Microbiological Diagnostics

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