Simultaneous analysis of naproxen, nabumetone and its major metabolite 6-methoxy-2-naphthylacetic acid in pharmaceuticals and human urine by high-performance liquid chromatography

Mikami, Eiichi; Goto, Tetsushi; Ohno, Tsutomu; Matsumoto, Hiroshi; Nishida, Mikio

doi:10.1016/s0731-7085(00)00365-4

Cited by 90 publications

(60 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several analytical methods have been reported for the determination of naproxen in pharmaceutical preparations including UV-visible spectrophotomery, [3][4][5] spectrofluorimetry, [6][7][8] room temperature phosphorimetry, 9,10 voltametry, 11 high-performance liquid chromatography, [12][13][14] capillary electrophoresis, 15,16 coulometry 17 and oscilometric titration. 18 However, most of these techniques are timeconsuming, involving the use of organic solvents or require expensive and sophisticated instruments and for this reason they are not suitable for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

A novel potentiometric naproxenate ion sensor immobilized in a graphite matrix for determination of naproxen in pharmaceuticals

Santini¹,

Oliveira²,

Pezza³

et al. 2006

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

As características, o desempenho e a aplicação de um eletrodo do tipo Pt⎪Hg⎪Hg 2 (NAP) 2 ⎪ Graphite, onde NAP=íon naproxenato, são descritas. O eletrodo responde a NAP com sensibilidade de (58,1± 0,9) mV década -1 no intervalo de 5,0 × 10 -5 -1,0 × 10 -2 mol L -1 , a pH 6,0-9,0 e com um limite de detecção de 3,9 × 10 -5 mol L -1 . O eletrodo é de baixo custo e facilmente construído, apresenta um rápido tempo de resposta (10-35 s) e pode ser usado por um período de seis meses sem qualquer divergência considerável nos potenciais. O sensor supracitado mostrou boa seletividade para naproxeno na presença de várias substâncias, tais como carboxilatos e ânions inorgânicos, sendo aplicado na análise direta de naproxeno em medicamentos (comprimidos) via método da adição de padrão. Os resultados analíticos obtidos com o eletrodo proposto estão em boa concordância com aqueles obtidos pelo procedimento preconizado na Farmacopéia Americana.The characteristics, performance, and application of an electrode, namely, Pt⎪Hg⎪Hg 2 (NAP) 2 ⎪ Graphite, where NAP stands for naproxenate ion, are described. This electrode responds to NAP with sensivity of (58.1± 0.9) mV decade -1 over the range 5.0 × 10 -5 -1.0 × 10 -2 mol L -1 at pH 6.0-9.0 and a detection limit of 3.9 × 10 -5 mol L -1 . The electrode is easily constructed at a relatively low cost with fast response time (within 10-35 s) and can be used for a period of 6 months without any considerable divergence in potentials. The proposed sensor displayed good selectivity for naproxen in the presence of several substances, especially concerning carboxylate and inorganic anions. It was used for the direct assay of naproxen in commercial tablets by means of the standard additions method. The analytical results obtained by using this electrode are in good agreement with those given by the United States Pharmacopeia procedure.Keywords: naproxenate-sensitive electrode, potentiometry, pharmaceutical formulations Introduction Naproxen [(+)-2-(6-metoxy-2naphthyl)propionic acid], is a non-steroidal anti-inflammatory drug that also presents analgesic and antipyretic properties often preferred to acetylsalicylic acid because of its better absortion following oral administration and fewer adverse effects.Naproxen is extensively used in the treatment of many diseases like rheumatoid arthrits, degenerative joint disease, ankylosing spondylits, acute gout and primary dismenorrea. 1 Like other non-steroidal anti-inflammatory drugs, it inhibits the biosyntesis of prostaglandins. 1 The United States Pharmacopeia 2 2003 reports an highperformance liquid chromatography (HPLC) method for the determination of naproxen tablets. Several analytical methods have been reported for the determination of naproxen in pharmaceutical preparations including UV-visible spectrophotomery, 3-5 spectrofluorimetry, 6-8 room temperature phosphorimetry, 9,10 voltametry, 11 high-performance liquid chromatography, 12-14 capillary electrophoresis, 15,16 coulometry 17 and oscilometric titration. 18 However, most of these techniq...

show abstract

Section: Introductionmentioning

confidence: 99%

A novel potentiometric naproxenate ion sensor immobilized in a graphite matrix for determination of naproxen in pharmaceuticals

Santini¹,

Oliveira²,

Pezza³

et al. 2006

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…The main target of the chromatographic method is to get the separation for closely eluting degradation products, mainly for the degradation product at 3.18 RT, which is eluting very closely to the NS. The degradation samples were run using different stationary phases like C 18 , C 8 and Mobile phases containing buffers like phosphate and acetate with different pH (2-7) and using organic modifiers like acetonitrile and methanol in the mobile phase. But the separation was satisfactory in the adopted chromatographic conditions only.…”

Section: Methods Development and Optimization Of Stability Indicating mentioning

confidence: 99%

“…So far, several liquid chromatography procedures have been described for the determination of SS and NS [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. But, these procedures were developed to estimate either SS or NS individually Page 2 of 6 and in combination with other drugs from formulation, plasma, urine, intestinal perfusion samples and in bulk drugs.…”

Section: Introductionmentioning

confidence: 99%

Rapid Simultaneous Determination of Sumatriptan Succinate and Naproxen Sodium in Combined Tablets by Validated Ultra Performance Liquid Chromatographic Method

Reddy¹,

Kumar²,

Reddy³

et al. 2011

J Anal Bioanal Techniques

View full text Add to dashboard Cite

A stability-indicating ultra perfomance liquid chromatography (UPLC) method was developed for the simultaneous determination of sumatriptan succinate and Naproxen sodium in pharmaceutical dosage forms. The chromatographic separation was achieved on C18, 50 mm × 4.8 mm, 1.8-µm particle size column. The mobile phase contains a mixture of 0.2% ortho phosphoric acid and acetonitrile as the mobile phase in gradient elution technique. The retention time of Sumatriptan and Naproxen was found to be 1.7 and 2.7 min respectively. The total runtime was 5 min within which two active compounds and degradation products were separated. This method allows the determination of 850-2565 µg mL -1 of sumatriptan succinate and 5000-15000 µg mL -1 of Naproxen sodium. The flow rate was 1.0 mL min -1 and the detection wavelength was 225 nm. The limit of detection (LOD) for sumatriptan succinate and Naproxen sodium was 1.9 and 1.5 µg mL -1 , respectively. The limit of quantification (LOQ) for sumatriptan succinate and Naproxen sodium was 6.3 and 4.8 µg mL -1 , respectively. This method was validated for accuracy, precision, linearity and robustness. sumatriptan succinate and Naproxen sodium were subjected to different ICH prescribed stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation and the method was also found to be stability indicating. Ultra performance liquid chromatography (UPLC) is a recent technique in liquid chromatography, which enables significant reductions in separation time and solvent consumption. Literature indicates that UPLC system allows about ninefold decrease in analysis time as compared to the conventional HPLC system using 5μm particle size analytical columns, and about threefold decrease in analysis time in comparison with 3μm particle size analytical columns without compromise on overall separation [23][24][25][26][27]. Rapid Simultaneous Determination of SumatriptanHence, a rapid, accurate stability indicating UPLC method for the simultaneous determination of SS and NS from combined dosage form was developed and validated. Experimental Instrumentation and chromatographic conditionsThe Waters UPLC Acquity system we used consists of a binary solvent manager, a sample manager and a UV detector. Zorbax SB C-18, 50 mm x 4.6 mm i.d with 1.8µm particles was used as stationary phase. 0.2% ortho phosphoric acid in water (pH 2.2) as solvent A and acetonitrile and water in the ratio 90:10; v/v, was as solvent B used for mobile phase. Prior to use, the mobile phase was mixed thoroughly and degassed. ReagentsStandards were supplied by D.C.O. Hyderabad, India. HPLC grade acetonitrile, analytical grade ortho phosphoric acids were purchased from Merck (Mumbai, India). Water was prepared by Millipore MilliQ Plus water purification system. Commercial pharmaceutical preparation of Triximet combined tablets were purchased from the market. The declared content of tablets was Sumatriptan 85 mg and Naproxen 500 mg per tablet. Preparation of solutionsStandard solutions: A standard so...

show abstract

“…They showed reduced resolution and it hampered their accurate determination. The addition of HSA, the hydrophobic ionpair reagent, is particularly useful for the separation and detection of several drugs, such as nabumetone 15) and mosapride, 16) because it provides appropriate capacity factors for complexes formed with the counterion. When the effects of HSA concentration were examined, as shown in Fig.…”

Section: Optimization Of the Chromatographic Systemmentioning

confidence: 99%

Simultaneous Analysis of Seven Benzodiazepines in Dietary Supplements as Adulterants Using High Performance Liquid Chromatography and its Application to an Identification System for Diazepam

Mikami

Goto

Ohno

et al. 2005

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

Simultaneous analysis of naproxen, nabumetone and its major metabolite 6-methoxy-2-naphthylacetic acid in pharmaceuticals and human urine by high-performance liquid chromatography

Cited by 90 publications

References 15 publications

A novel potentiometric naproxenate ion sensor immobilized in a graphite matrix for determination of naproxen in pharmaceuticals

A novel potentiometric naproxenate ion sensor immobilized in a graphite matrix for determination of naproxen in pharmaceuticals

Rapid Simultaneous Determination of Sumatriptan Succinate and Naproxen Sodium in Combined Tablets by Validated Ultra Performance Liquid Chromatographic Method

Simultaneous Analysis of Seven Benzodiazepines in Dietary Supplements as Adulterants Using High Performance Liquid Chromatography and its Application to an Identification System for Diazepam

Contact Info

Product

Resources

About