Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Abstract

Phytohormones have attracted wide attention due to their important biological functions. However, their detection is still a challenge because of their complex composition, low abundance and diverse sources. In this study, a novel method of high-performance liquid chromatography with electrospray ionization tandem mass spectrometry was developed and validated for the simultaneous determination of ten phytohormones including indole-3-acetic acid, isopentenyladenine, isopentenyl adenosine, trans-zeatin riboside, zeatin, strigolactones, abscisic acid, salicylic acid, gibberellin A3, and jasmonic acid in Sargassum horneri (S. horneri). The phytohormones were extracted from freeze-dried S. horneri with methanol/water/methanoic acid (15:4:1, v/v/v) analyzed on a Hypersil Gold C18 column and detected by electrospray ionization tandem triple quadrupole mass spectrometry in the multiple reaction monitoring mode. The experimental conditions for the extraction and analysis of phytohormones were optimized and validated in terms of reproducibility, linearity, sensitivity, recovery, accuracy, and stability. Distributions of the phytohormones in the stems, blades, and gas bladder of the S. horneri in drift, fixed, and semi-fixed growing states were investigated for the first time. The observed contents of the phytohormones in S. horneri range from not detected to 5066.67 ng/g (fresh weight). Most phytohormones are distributed mainly in the stems of S. horneri in drift and semi-fixed states.

Combined BSA-Seq Based Mapping and RNA-Seq Profiling Reveal Candidate Genes Associated with Plant Architecture in Brassica napus

“…A total of 36 tall and 36 dwarf individuals in the BC 8 population were used for a phytohormone analysis, with three biological replicates (each replicate contains 12 individuals). By following a previous study [ 45 ], the phytohormones IAA, GA, ABA, SL, CK, and ETH were extracted. Briefly, the freeze-dried tissues were crushed in a mixer mill (30 Hz, 1 min).…”

Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis

“…The method for phytohormone detection followed the reported procedures with modification [ 56 , 57 ]. Five hundred milligrams of frozen fresh N. haitanensis samples were ground to powder using liquid nitrogen and then extracted with 5 mL MeOH: H 2 O: HCOOH (15: 4: 1) containing 0.5% butylated hydroxytoluene by ultrasonication for 10 min.…”

Comprehensive Analysis of Long Non-coding RNA Modulates Axillary Bud Development in Tobacco (Nicotiana tabacum L.)

“…The mixture was scrolled and extracted by a refrigerated centrifuge at 12,000 r/min for 5 min. The concentrated solution was re-dissolved in 100 μl of methanol (80% v/v), using a 0.22 μM ultrafiltrate membrane for LC–MS/MS analysis ( Floková et al, 2014 ; Li Y. et al, 2016 ). UPLC-ESI-MS/MSsystem (UPLC’ExionLC ™ AD, https://sciex.com.cn/ ; MS, Applied Biosystems 6500 Triple Quadrupole, https://sciex.com.cn/ ) was used for separation and quantification.…”