Simultaneous analysis of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography–tandem mass spectrometry

Han, Eunyoung; Park, Yonghoon; Kim, Eun‐Mi; In, Sangwhan; Yang, Wenjun; Lee, Sooyeun; Choi, Hwakyung; Lee, Sangki; Chung, Heesun; Song, Joon Myong

doi:10.1016/j.jpba.2011.03.020

Cited by 37 publications

(32 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we approach uncertainty factor in another aspect in this study because our analysis is for hair analysis and hair is a solid matrix. We spike standard solutions in matrix (blank hair samples) and then the extraction efficiency was good as described in the previous studies [22,23], therefore we excluded the uncertainty from method bias. As suggested by Lee et al [18], in case of hair analysis, they also excluded the method bias study while the method bias study should be evaluated for in-house method in blood or urine.…”

Section: Discussionmentioning

confidence: 99%

“…Method precision was investigated by analyzing hair samples (20 mg) spiked with medium (0.08 ng) concentrations of THCCOOH as described in the previous studies [22,23].…”

Section: Evaluation Of Methods Precisionmentioning

confidence: 99%

“…Each triplicate hair sample (20 mg) was washed two times with 1 mL of methanol in order to remove external contamination. Sample extraction was performed using the methods described in previous studies [22,23]. Briefly, the hair samples were digested with 1 mL of 1 M NaOH at 85 8C for 30 min in the presence of internal standards of THCCOOH-d 3 (2.5 pg/mg).…”

Section: Sample Collection and Extraction Protocolmentioning

confidence: 99%

“…The limit of detection (using a 20-mg hair sample) was 0.025 pg/mg with a signal-to-noise ratio >3 for THCCOOH and calibration curves were performed using drug-free controlled hair. The cut-off value for THCCOOH in hair was 0.05 pg/mg by GC/MS/MS-NCI confirmation [22][23][24]. The experimental procedure was fully validated in previous studies [22,23].…”

Section: Instrumentationmentioning

confidence: 99%

“…The cut-off value for THCCOOH in hair was 0.05 pg/mg by GC/MS/MS-NCI confirmation [22][23][24]. The experimental procedure was fully validated in previous studies [22,23].…”

Section: Instrumentationmentioning

confidence: 99%

See 4 more Smart Citations

Establishment of the measurement uncertainty of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid in hair

Han

Yang

Lee

et al. 2011

Forensic Science International

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…Method precision was investigated by analyzing hair samples (20 mg) spiked with medium (0.08 ng) concentrations of THCCOOH as described in the previous studies [22,23].…”

Section: Evaluation Of Methods Precisionmentioning

confidence: 99%

Section: Sample Collection and Extraction Protocolmentioning

confidence: 99%

Section: Instrumentationmentioning

confidence: 99%

“…The cut-off value for THCCOOH in hair was 0.05 pg/mg by GC/MS/MS-NCI confirmation [22][23][24]. The experimental procedure was fully validated in previous studies [22,23].…”

Section: Instrumentationmentioning

confidence: 99%

See 3 more Smart Citations

Establishment of the measurement uncertainty of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid in hair

Han

Yang

Lee

et al. 2011

Forensic Science International

Self Cite

View full text Add to dashboard Cite

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Battista

Sergi

Montesano

et al. 2013

Drug Testing and Analysis

View full text Add to dashboard Cite

Over the last two decades, the role played by phytocannabinoids and endocannabinoids in medicine has gained increasing interest in the scientific community. Upon identification of the plant compound Δ(9)-tetrahydrocannabinol (THC) and of the endogenous substance anandamide (AEA), different methodological approaches and innovative techniques have been developed, in order to evaluate the content of these molecules in various human matrices. In this review, we discuss the analytical methods that are currently used for the identification of phytocannabinoids and endocannabinoids, and we summarize the benefits and limitations of these procedures. Moreover, we provide an overview of the main biological matrices that have been analyzed to date for qualitative detection and quantitative determination of these compounds.

show abstract

Simultaneous quantification of THC‐COOH, OH‐THC, and further cannabinoids in human hair by gas chromatography–tandem mass spectrometry with electron ionization applying automated sample preparation

Kieliba

Lerch

Andresen‐Streichert

et al. 2018

Drug Testing and Analysis

View full text Add to dashboard Cite

The detection of Δ -tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair, for the purpose of identifying cannabis consumption, is conducted in many forensic laboratories. Since external contamination of hair with these cannabis components cannot be excluded, even after hair decontamination, only the detection of THC metabolites such as 11-nor-9-carboxy-Δ -tetrahydrocannabinol (THC-COOH) or 11-hydroxy-Δ -tetrahydrocannabinol (OH-THC), is considered to prove cannabis consumption. At present, testing for THC metabolites is not standard practice due to its analytical complexity. For these reasons, we developed a novel method for the detection of THC-COOH and OH-THC as well as THC, CBD, and CBN in one single analytical run using gas chromatography-tandem mass spectrometry (GC-MS/MS) with electron ionization. After manual hair washing and grinding, sample preparation was fully automated, by means of a robotic autosampler. The hair extraction took place by digestion with sodium hydroxide. A solid-phase extraction (SPE) was chosen for sample clean-up, using a mixed-mode anion exchange sorbent. Derivatization of all analytes was by silylation. The method has been fully validated according to guidelines of the Society of Toxicological and Forensic Chemistry (GTFCh), with a limit of detection (LOD) of 0.2 pg/mg for THC-COOH and OH-THC and 2 pg/mg for THC, CBD and CBN, respectively, thus fulfilling the Society of Hair Testing (SoHT) recommendations. The validated method has been successfully applied to our routine forensic case work and a summary of data from authentic hair samples is given, as well as data from proficiency tests.

show abstract

Simultaneous analysis of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography–tandem mass spectrometry

Cited by 37 publications

References 29 publications

Establishment of the measurement uncertainty of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid in hair

Establishment of the measurement uncertainty of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid in hair

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Simultaneous quantification of THC‐COOH, OH‐THC, and further cannabinoids in human hair by gas chromatography–tandem mass spectrometry with electron ionization applying automated sample preparation

Contact Info

Product

Resources

About