Simultaneous and rapid analysis of chemical preservatives in processed animal products by ultra-performance liquid chromatography

Abstract

An ultra-performance liquid chromatography-tunable ultraviolet method was optimized and validated for the simultaneous analysis of nine chemical preservatives in processed animal products. The limits of detection and quantification for the preservatives were within the ranges of 0.02-0.23 and 0.07-0.76 μg/mL, respectively. The relative standard deviations for intraday analyses of retention time and peak area were 0.00-0.23 and 0.03-2.93%, respectively, whereas, those for interday analyses were 0.67-2.30 and 2.12-5.37%, respectively. Of the nine preservatives spiked into six different animal products, dehydroacetic acid spiked into soft cheese exhibited the lowest recovery rate of 72.1 ± 0.36% at the lowest concentration (0.25 g/kg). Comparing data between UPLC and high-performance liquid chromatography with a 5% significance level, the t-statistic was 1.42. Moreover, sorbic acid was detected in 16 animal products (0.11-2.49 g/kg) when 278 products were analyzed for preservatives.

Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37

“…The increase in the use of chemical antiseptics has led to increased public discussion. The misuse of chemical antiseptics not only results in public health risks such as allergy and carcinogenesis, but also endangers the environment [23]. In seeking replacements to apply for crop protection, we found that A. niger, a fungus used in the food industry, has enormous antibacterial activity against A. tumefaciens T-37, which is a species of major concern as a plant pathogen [6].…”

An environment-friendly approach using deep eutectic solvent combined with liquid–liquid microextraction based on solidification of floating organic droplets for simultaneous determination of preservatives in beverages

“…Parabens are alkyl esters of p-hydroxybenzoic acid, which are commonly used as chemical preservatives in food, cosmetics and pharmaceuticals. 1,2 Shinshi Oishi has demonstrated that butyl paraben (BP) has a harmful effect on the reproductive system of male mice by damaging spermatogenesis. 3 Methyl paraben (MP), ethyl paraben (EP), propyl paraben (PP), and BP are estrogenic-like substances 4 and high exposure to estrogenic-like substances might lead to breast cancer.…”

Stability indicating reversed‐phase‐high‐performance liquid chromatography method development and validation for pyridostigmine bromide and sodium benzoate in oral solution

“…Consequently, the estimation of the drug product is essential in the regulated pharmaceutical industry. To date, several analytical methods, primarily for the estimation of PGB (Abu‐Qare & Abou‐Donia, 2001; Barber et al, 1979; Ellin et al, 1982; Giovanni et al, 2019; Matsunaga et al, 1987) and SBN (Abdollahpour et al, 2010; Boukarim et al, 2009; Can et al, 2011; Ettaboina, Katakam, & Dongala, 2022; Ettaboina, Nakkala, & Chathalingath, 2022; Galli & Barbas, 2004; Hewala, 1994; Jwahaeng et al, 2018; Katakam, Ettaboina, & Dongala, 2021; Katakam, Ettaboina, & Marisetti, 2021; Lakka et al, 2021, 2022; Lakka & Goswami, 2012; Narasimha et al, 2011; Narasimha & Chandrasekar, 2019; Navneet et al, 2012; Parvateesam et al, 2023; Pylypiw & Grether, 2000; Shabir, 2011; Siva et al, 2022), have been developed using various analytical techniques. None of the methods are inherently stability‐indicating for the estimation of PGB and SBN in liquid oral dosage forms.…”