Simultaneous and rapid differential diagnosis of Mycoplasma genitalium and Ureaplasma urealyticum based on a polymerase chain reaction-restriction fragment length polymorphism

Mirnejad, Reza; Amirmozafari, Nour; Kazemi, Bahram

doi:10.4103/0255-0857.76521

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…Usually, PCR is used to detect M. genitalium infections, because it has proved to be more sensitive, specific, and rapid than the conventional microbiology methods 8, 19. For example, detection of M. genitalium by the culture method requires up to 8 weeks 9, 20.…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of M. genitalium was 12% and 2% in symptomatic and asymptomatic men, respectively, and M. genitalium infection was associated with dysuria, testis inflammation, pelvic pain, and low educational level [4]. In Mirnejad et al's [20] study, genital swabs were taken from 210 women in Tehran, Iran, and, using PCR the frequency of M. genitalium was 3.3%. The prevalence of C. trachomatis and M. genitalium in pregnant women of Sabzevar (north-east of Iran) in Haghighi Hasanabad et al's [9] study was determined in 196 urine specimens by duplex PCR.…”

Section: Discussionmentioning

confidence: 99%

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A Case–control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction

Ramazanzadeh

Khodabandehloo

Farhadifar

et al. 2016

Osong Public Health and Research Perspectives

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ObjectivesMycoplasma genitalium infections are suggested as causes of a number of pathological outcomes in pregnant women. The aim of this study was to evaluate the frequency of M. genitalium infections among pregnant women and its association with spontaneous abortion.MethodsIn this case–control study we included 109 women with spontaneous abortion with a gestational age of 10–20 weeks (patients), and 109 women with normal pregnancy with a gestational age of 20–37 weeks (controls) in Sanandaj, Iran. Using specific primers and extracted DNA from endocervical swabs, a polymerase chain reaction was conducted for the detection of M. genitalium infection in both groups.ResultsThe frequency of M. genitalium infection in patient and control groups was one (0.91%) and three (2.75%), respectively. In both control and patient groups using Fisher test, no association between mycoplasma infection and spontaneous abortion was seen.ConclusionM. genitalium may be positive in the genital tract of some pregnant women but was not associated with spontaneous abortion. Further powerful studies with larger sample sizes are needed for the determination of a possible role of M. genitalium in pregnancy outcomes and spontaneous abortion.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Case–control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction

Ramazanzadeh

Khodabandehloo

Farhadifar

et al. 2016

Osong Public Health and Research Perspectives

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“…With better laboratory detection and increasing knowledge about their significance in maternal and neonatal infections, this situation is now changing (8), as high as 40 to 80% of women have been reported to be colonized with genital mycoplasmas (9). Their role in infertility, post-partum endometritis, chorioamnionitis, preterm labor, and spontaneous abortion has been extensively investigated.…”

Section: Discussionmentioning

confidence: 99%

Ureaplasma Urealyticum and Mycoplasma Hominis Colonization and Chronic Lung Disease in Preterm Neonates Under Ventilation

et al. 2013

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A B S T R A C TBackground: Ureaplasma urealyticum (U. urealyticum) and Mycoplasma hominis (M. hominis) are two important pathogens in neonatal respiratory tract infections. As low gestational age and birth weight increase the colonization rate with these pathogens, and they are also risk factors of chronic lung disease (CLD), it is difficult to establish a statistically significant relation between these two factors. Objectives: To determine the colonization rate of preterm ventilated neonates with U. urealyticum and M. hominis , and the relationship between infection and chronic lung disease in these neonates, a prospective study was performed. Patients and Methods: Determining tracheal colonization rate of preterm neonates with ureaplasma and mycoplasmsa and its relation to CLD. In a cohort prospective study in Hazrat Rasoul Akram Hospital, with 62 ventilated neonates (< 35 weeks) in the first 24 h of life, where tracheal secretions were aspirated, transported, and cultured in a specific media. CLD was diagnosed as oxygen requirement at 28 days or 36 weeks post conceptional age. Results: From 62 cultures, 7 were positive for U. urealyticum (11.3%, CI95%: 3.4-19.2%) and 3 were positive for M. hominis (4.8%, CI95%: 0-10.2%). From 53 neonates who were alive at 28 days after birth, 11 neonates needed oxygen (20.8%, CI95%: 9.8-31.7%). From 10 neonates with positive cultures, 2 (20%, CI95%: 0-44.8%) died, and from 8 alive neonates, 3 (37.5%, CI95%: 4.0-71.0%) survived without CLD. From 52 neonates with negative cultures, 7 (13.5%, CI95%: 4.2-22.7%) died, and from 45 alive neonates, 39 (86.7%, CI95%: 76.7-96.6%) survived without CLD, and there was a significant difference between neonatal groups with positive and negative tracheal culture progressing to CLD (62.5% vs. 13.3%) (P < 0.001) (Figure 1). Conclusions: Intrauterine infection with U. urealyticum and M. hominis leads to negative neonatal outcome and increased rate of chronic lung disease in preterm neonates (< 35 weeks).

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“…Other detection methods were developed based on PCR. Mirnejad et al [7] used PCR restrictive fragment length polymorphism (RFLP) to simultaneously detect and identify Mg and ureaplasma urealyticum (Uu). By designing specific primers and restriction enzymes, PCR-RFLP provides a simple, fast, and accurate method for Mg and Uu clinical tests and possible means for other genotyping studies.…”

Section: Pcr Technologymentioning

confidence: 99%

Advances in Epidemiological Studies of Herpes Zoster

Gu¹

2015

Infection International

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Mycoplasma genitalium (Mg) commonly causes nongonococcal urethritis and cervicitis. Mg is a fastidious bacterium that poses difficulty in time-consuming isolation and culture. Lack of specificity for serological tests also hampers clinical research of Mg. With development of molecular biology, polymerase chain reaction tests, which exhibit high sensitivities and specificities, became primary tools for foundational and clinical studies of Mg.

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Simultaneous and rapid differential diagnosis of Mycoplasma genitalium and Ureaplasma urealyticum based on a polymerase chain reaction-restriction fragment length polymorphism

Abstract: PCR-RFLP offers a rapid and easily applicable protocol to simultaneous detection and differentiation of M. genitalium and U. urealyticum from clinical samples when specific primers and restriction enzymes are used.

Cited by 10 publications

References 17 publications

A Case–control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction

A Case–control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction

Ureaplasma Urealyticum and Mycoplasma Hominis Colonization and Chronic Lung Disease in Preterm Neonates Under Ventilation

Advances in Epidemiological Studies of Herpes Zoster

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