Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity

Karuppiah, Valliappan; Vallikkannu, Murugappan; Chen, Jie

doi:10.1186/s12934-019-1233-7

Cited by 42 publications

(24 citation statements)

References 59 publications

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“…6). like T. asperellum GDFS1009 for Fusarium graminearum (Karuppiah et al 2019), and T. harzianum INAT11 for Fusarium verticillioides and Fusarium graminearum (Ferrigo et al 2020). The ameliorative role of Trichoderma under abiotic stress has been also confirmed by many investigations like treating wheat with Trichoderma longibrachiatum under salinity (10 and 20 g l −1 NaCl) as published by .…”

Section: Trichoderma Under Stressmentioning

confidence: 72%

Sustainable Approaches of Trichoderma under Changing Environments for Vegetable Production

Taha

Kamel

Elsakhawy

et al. 2020

EBSS

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T HE world's burgeoning population faces a great challenge concerning food security, which could be achieved through different sustainable agricultural practices. Trichoderma, as a ubiquitous fungus, is one of the most promising microorganisms that might offer several avenues for sustainable agriculture. Trichoderma spp. may guarantee a better solution for conventional problems in agriculture through several approaches including the protection of cultivated plants from undesirable abiotic and biotic conditions under changing environments and promoting their growth in poor or limited soil nutrients. The promising role of Trichoderma for vegetable production as a biocontrol and biofertilizers has been confirmed but its role as a plant pathogen still needs more studies. Trichoderma could inhibit or suppress the growth of soil phytopathogens, promoting plant growth and soil health, through activation of many mechanisms including synthesis of antibiotics, mycoparasitism and competition for nutrients and space against plant deleterious microorganisms. The sustainable approaches of Trichoderma including biofortification, bio-remediation and phyto-remediationas well as exploring future research opportunities will be also addressed in this work.

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Section: Trichoderma Under Stressmentioning

confidence: 72%

Sustainable Approaches of Trichoderma under Changing Environments for Vegetable Production

Taha

Kamel

Elsakhawy

et al. 2020

EBSS

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“…The co-cultivation with B. amyloliquefaciens offered differentiated cellular environs to induce the genes involved in metabolic pathways [11,12]. The metabolic pathway might comprise several enzymes, and these properties could vary depending on the circumstance.…”

Section: Discussionmentioning

confidence: 99%

“…The present investigations revealed that the overexpression of the Vel1 gene might induce the secondary metabolite producing genes of Trichoderma and mycoparasitism to upregulate the defense potential of plants. Previously, Karuppiah et al [12] evidenced that the co-culture stimulated the defense potential of plants against pathogens. Likewise, we observed that the TA OE-Vel1 + BA and TA + BA co-culture is improving the plant defense gene expression than the axenic cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Strand cDNA Synthesis Kit (+gDNA wiper). The ampli cation was performed as described previously by [12] using Roche light cycler 96. The Actin gene was used to normalize the gene expression.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we proved that the co-cultivation of B. amyloliquefaciens 1841 and T. asperellum GDFS1009 activated several genes and induced the production of secondary metabolites and enzymes, including cellulase [11,12]. B. amyloliquefaciens used in the co-cultivation is a plant growth promoting rhizobacteria, which stimulated the plant growth and bio-control mechanism [11][12][13]. The role of T. atrovirideVel1 was enhanced by the B. amyloliquefaciens in the co-culture [9].…”

Section: Introductionmentioning

confidence: 92%

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Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

Karuppiah

Liu

et al. 2020

Preprint

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Background: Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results: The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion: The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.

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Characterization of the Synergistic Effect of Fungal Isolates in Suppressing Ganoderma boninense and Enhancing Oil Palm Growth

Anuar,

Hashim,

Sundram

et al. 2024

Journal of Basic Microbiology

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The globally vital oil palm, a major oil producer, confronts productivity challenges due to Ganoderma boninense (Gb), causing output decline. Chemical control efforts have proven ineffective, prompting exploration of microbial‐based biocontrol. While single fungal biocontrol research exists, the impact of employing multiple biocontrols concurrently to combat Ganoderma and enhance oil palm growth remains uncharted. This study examined four soil‐derived fungal isolates for their ability to antagonize Gb PER71 in vitro. Molecular identification categorized them as Talaromyces spp. and Penicillium sp. Moreover, all isolates were revealed to have at least three plant growth‐promoting (PGP) traits and were shown to have phosphoric hydrolase, ester hydrolase, peptide hydrolase, and glycosidase activities which are essential for plant growth. Furthermore, the synergistic evaluation of fungal isolates was tested against Gb PER71. One out of six combinations of fungal isolates showed a synergistic effect in vitro, and two showed a synergistic effect in planta. The application of single and combined fungal isolates tested in planta also suppressed Gb PER71 and enhanced oil palm growth compared to control groups. The findings indicate the promising potential of these isolates as biocontrol agents (BCAs) and bioformulations against Gb in oil palm cultivation.

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Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity

Cited by 42 publications

References 59 publications

Sustainable Approaches of Trichoderma under Changing Environments for Vegetable Production

Sustainable Approaches of Trichoderma under Changing Environments for Vegetable Production

Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

Characterization of the Synergistic Effect of Fungal Isolates in Suppressing Ganoderma boninense and Enhancing Oil Palm Growth

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