Simultaneous Assay of Immunoreactive β-Lipotropin, γ-Lipotropin, and β-Endorphin in Plasma of Normal Human Subjects, Patients with ACTH/Lipotropin Hypersecretory Syndromes, and Patients undergoing Chronic Hemodialysis

Bertagna, Xavier; Stone, William J.; Nicholson, Wendell E.; Mount, Charles D.; Orth, David N.

doi:10.1172/jci110004

Cited by 43 publications

(8 citation statements)

References 52 publications

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“…The hBend RIA was performed as described (35) (37), slightly modified in our laboratory (38). Briefly, frozen tissues were ground in liquid nitrogen, then homogenized at 4VC in a Waring blender in 20 volumes (wt/vol) of prechilled 0.02 M sodium acetate buffer, pH 5, containing 7 M guanidine-HCl.…”

Section: Methodsmentioning

confidence: 99%

Altered proopiomelanocortin gene expression in adrenocorticotropin-producing nonpituitary tumors. Comparative studies with corticotropic adenomas and normal pituitaries.

Keyzer¹,

Bertagna²,

Lenne³

et al. 1985

J. Clin. Invest.

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In order to assess the mechanisms of proopiomelanocortin (POMC) gene expression in human ACTH-producing tumors, we performed the simultaneous evaluation of POMC products and messenger RNA (mRNA) in tissue fragments obtained from two corticotropic adenomas, five nonpituitary tumors, and two normal human pituitaries. The POMC products were examined using a combination of gel exclusion chromatography and four different radioimmunoassays directed against "3 melanocyte stimulating hormone (y3MSH), ACTH, 'y-lipotropin ('yLPH), and f-endorphin. The POMCmRNA was detected and analyzed by dot and northern blot hybridization using a single-stranded genomic DNA probe corresponding to the coding region of the human POMC gene. Tissue concentrations of POMC products and mRNA showed parallel distributions. Immunoreactive It is concluded that POMC gene expression appears qualitatively unaltered in corticotropic adenomas. In nonpituitary tumors, in contrast, abnormal POMC processing is frequent; in addition, an extra POMCmRNA was detected in a thymic tumor with a greater length than the normal mRNA; the mechanisms and pathophysiological implications of these modifications remain to be elucidated.

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Section: Methodsmentioning

confidence: 99%

Altered proopiomelanocortin gene expression in adrenocorticotropin-producing nonpituitary tumors. Comparative studies with corticotropic adenomas and normal pituitaries.

Keyzer¹,

Bertagna²,

Lenne³

et al. 1985

J. Clin. Invest.

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“…The arterial catheter was used to sample blood and the splenic vein was used to infuse insulin and glucagon at basal levels. Angiocaths sured by a radioimmunoassay using '25l-labeled tracer and rabbit antihBE antiserum (13).…”

Section: Methodsmentioning

confidence: 99%

Beta-endorphin inhibits glucose production in the conscious dog.

Radosevich¹,

Williams²,

McRae³

et al. 1984

J. Clin. Invest.

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Abstract. The effect of human fl-endorphin (h(3E) infusion (0.2 mg/h) on glucose homeostasis was studied in 10 conscious overnight fasted dogs in which endocrine pancreatic function was fixed at basal levels with somatostatin plus intraportal replacement of basal insulin and glucagon.h#E caused a fall in plasma glucose from 107±5 to 76±6 mg/dl by 3 h (P < 0.01). This was due to a 25% fall in tracer-determined glucose production (Ra; P < 0.01). A significantly larger fall in Ra was observed in four dogs in which hypoglycemia was prevented by use of an exogenous glucose infusion (45 vs. 25%, P < 0.05). These changes occurred in the absence of changes in circulating levels of insulin, glucagon, epinephrine, norepinephrine, and cortisol.We conclude that the naturally occurring opioid peptide, f3-endorphin, inhibits glucose production by the liver in vivo. This appears to be a direct effect of the opioid on the liver, since the inhibition took place in the absence ofchanges in the other hormones measured. These results suggest that endorphins act on glucose homeostasis in a complex way, both by affecting other glucoregulatory hormones as demonstrated elsewhere, and by directly modulating hepatic glucose production as shown here. IntroductionThe role of opioids in glucoregulation is not completely clear. Several reports (1-3) have shown that opioids can cause hy-

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“…After suppression of the pituitary hypersecretion by cortisol in a patient with Addison's disease, Bertagna et al (1981) observed a rapid fall in both immunoreactive 7-1ipotropin and immunoreactive fl-lipotropin including fl-endorphin. The initial and secondary half-lifes (tl/2) of 7-1ipotropin were 80 and 170min, and for fl-lipotropin/fl-endorphin 100 and 180min.…”

Section: Endogenous Peptidesmentioning

confidence: 97%

“…Although in the various studies a variety of doses has been used to assess the pharmacokinetic data, generally the half-life of fl-endorphin found is in the order of 20-50 min. There is a tendency that the half-life of fl-lipotropin is somewhat longer than that of fl-endorphin as indicated by studies on endogenous as well as exogenous fl-lipotropin and endorphins (Aronin et al, 1981;Bertagna et al, 1981). Slower disappearance rates are found after suppressing the pituitary secretion by cortisol.…”

Section: Exogenous Peptidesmentioning

confidence: 99%

Action of proteolytic enzymes on lipotropins and endorphins: Biosynthesis, biotransformation and fate

Burbach¹

1984

Pharmacology & Therapeutics

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Simultaneous Assay of Immunoreactive β-Lipotropin, γ-Lipotropin, and β-Endorphin in Plasma of Normal Human Subjects, Patients with ACTH/Lipotropin Hypersecretory Syndromes, and Patients undergoing Chronic Hemodialysis

Cited by 43 publications

References 52 publications

Altered proopiomelanocortin gene expression in adrenocorticotropin-producing nonpituitary tumors. Comparative studies with corticotropic adenomas and normal pituitaries.

Altered proopiomelanocortin gene expression in adrenocorticotropin-producing nonpituitary tumors. Comparative studies with corticotropic adenomas and normal pituitaries.

Beta-endorphin inhibits glucose production in the conscious dog.

Action of proteolytic enzymes on lipotropins and endorphins: Biosynthesis, biotransformation and fate

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