Simultaneous Assay of Olanzapine and Fluoxetine in Tablets by Column High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography

Abstract

This paper describes validated high-performance liquid chromatographic (LC) and high-performance thin-layer chromatographic (TLC) methods for the simultaneous estimation of olanzapine and fluoxetine in pure powder and tablet formulations. The LC separation was achieved on a Lichrospher 100 RP-180, C18 column (250 mm, 4.0 mm id, 5 m) using 0.05 M potassium dihydrogen phosphate buffer (pH 5.6 adjusted with o-phosphoric acid) acetonitrile (50 + 50, v/v) as the mobile phase at a flow rate of 1 mL/min and ambient temperature. The TLC separation was achieved on aluminum sheets coated with silica gel 60F254 using methanoltoluene (40 + 20, v/v) as the mobile phase. Quantitation was achieved by measuring ultraviolet absorption at 233 nm over the concentration range of 1070 and 40280 g/mL with mean recovery of 99.54 0.89 and 99.73 0.58% for olanzapine and fluoxetine, respectively, by the LC method. Quantitation was achieved by measuring ultraviolet absorption at 233 nm over the concentration range of 100800 and 4003200 ng/spot with mean recovery of 101.53 0.06 and 101.45 0.35% for olanzapine and fluoxetine, respectively, by the TLC method with densitometry. These methods are simple, precise, and sensitive, and they are applicable for simultaneous determination of olanzapine and fluoxetine in tablet formulations.

Determination of olanzapine for therapeutic drug monitoring in schizophrenia patients by LC/MS method

“…These studies include the methods of liquid chromatographic devices combined with ultraviolet (Boulton, Markowitz, & DeVane, 2001;D'Arrigo, Migliardi, Santoro, & Spina, 2006;Olesen, Poulsen, & Linnet, 2001;Sachse, Köller, Härtter, & Hiemke, 2006;Weigmann et al, 2001), diode array (Llorca et al, 2001;Saracino, Koukopoulos, Sani, Amore, & Raggi, 2007;Siva Selva Kumar & Ramanathan, 2016), amperometric (Raggi et al, 2000) and electrochemical detection systems (Aravagiri, Ames, Wirshing, & Marder, 1997;Kobylińska, Buś, & Bukowska-Kiliszek, 2008) and also mass spectrometric systems including MS (Cao, Zhang, Tian, Li, & Rui, 2012;Choong et al, 2009;Zhou et al, 2004) and mainly tandem mass (MS/MS) detectors (Ansermot, Brawand-Amey, Kottelat, & Eap, 2013;Berna, Ackermann, Ruterbories, & Glass, 2002;Cavalcanti Bedor et al, 2015;Kirchherr & Kühn-Velten, 2006;Nirogi et al, 2006;Ravinder, Bapuji, Mukkanti, & Reddy, 2013;Reddy, Reddy, Sreeramulu, & Kanumula, 2007;Uřinovská et al, 2012). Additionally, the use of gas chromatography coupled to nitrogen phosphorus detection (Robertson & McMullin, 2000;Ulrich, 2005), gas chromatography mass spectrometric detection (Da Fonseca et al, 2013;Ikeda et al, 2012) and high-performance thin-layer chromatography (Shah, Shah, Suhagia, & Patel, 2007;Wróblewski, Petruczynik, & Waksmundzka-Hajnos, 2014) have been reported. According to our literature survey, this study is the first study on the determination of olanzapine from schizophrenia patients after intramuscular injection.…”

Use of Sodium Tetraphenyl Boron for Fabrication of Potentiometric Membrane Sensor for the Assay of Olanzapine in Pharmaceuticals and Human Urine

“…In the literature titrimetry [1][2][3] , visible spectrophotometry [3][4][5][6][7][8][9][10] , kinetic spectrophotometry [11] , UV-spectrophotometry [2,12] , capillary zone electrophoresis and linear voltammetry [12] and high-performance thin layer chromatography (HPTLC) [13][14][15] have been reported for determination of OLP in pharmaceuticals. Several liquid chromatographic methods [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] have also been reported for the assay of OLP in pharmaceuticals and biological materials. analyses [34][35][36][37][38][39][40][41][42][43][44][45] .…”

Determination of fluoxetine in pharmaceutical and biological samples based on the silver nanoparticle enhanced fluorescence of fluoxetine–terbium complex

“…Therefore, developing a very sensitive analytical method for the determination of FLX is of great importance (4). Several methods have been reported for the quantitation of FLX in various samples including capillary electrophoresis (5), nuclear magnetic resonance spectrometry (6), chromatography (7)(8)(9), chromatography-mass spectrometry (4,10,11), electrochemical methods (12,13), spectrophotometry (14)(15)(16) and spectrofluorimetry (2,17,18). Many of these methods have some major drawbacks such as lack of selectivity, tedious extraction procedures and time-consuming procedures.…”