Simultaneous assessment of nitrification and denitrification on freshwater epilithic biofilms by acetylene block method

Teissier, Samuel; Torre, M.

doi:10.1016/s0043-1354(02)00098-2

Cited by 35 publications

(22 citation statements)

References 24 publications

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“…1), indicating that ammonium availability had a stimulating effect on both the establishment and the intensity of biofilm-associated nitrification activity, as previously reported (3). Maximum rates were similar to activities measured at the oxic/anoxic interface of river sediments (18 to 45 nmol cm Ϫ2 h Ϫ1 ) (7) but in the lower range of activities reported from river and estuary biofilms (10 to 171 nmol cm Ϫ2 h Ϫ1 ) (13,19,20,38). Community composition and abundance of ammonia oxidizers.…”

supporting

confidence: 80%

Ammonium Availability Affects the Ratio of Ammonia-Oxidizing Bacteria to Ammonia-Oxidizing Archaea in Simulated Creek Ecosystems

Herrmann

Scheibe

Avrahami

et al. 2011

Appl Environ Microbiol

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The ammonia-oxidizing microbial community colonizing clay tiles in flow channels changed in favor of ammonia-oxidizing bacteria during a 12-week incubation period even at originally high ratios of ammoniaoxidizing archaea to ammonia-oxidizing bacteria (AOB). AOB predominance was established more rapidly in flow channels incubated at 350 M NH 4 ؉ than in those incubated at 50 or 20 M NH 4 ؉ . Biofilm-associated potential nitrification activity was first detected after 28 days and was positively correlated with bacterial but not archaeal amoA gene copy numbers.Nitrogen transformation processes in small-river ecosystems are primarily associated with the sediment or epilithic biofilms (4,5,18). Here, nitrification as the first step in coupled nitrification-denitrification is a key step in the removal of the allochthonous nitrogen load, preventing the eutrophication of downstream ecosystems (1,5,29,39). The activity and community composition of biofilm-associated nitrifying microorganisms have been investigated extensively in wastewater treatment systems (27,36), in drinking water distribution systems (17, 41), and in lakes, large rivers, and estuaries (7,19,21,39) but only rarely in small-creek ecosystems. The first and ratelimiting step in nitrification, oxidation of ammonia, is carried out by ammonia-oxidizing archaea (AOA) (14) and ammoniaoxidizing bacteria (AOB) (16). Molecular surveys targeting the amoA gene, which encodes catalytic subunit A of ammonia monooxygenase, the key enzyme of ammonia oxidation (33), as well as cultivation-based studies, indicated that pH (26), salinity (25, 34), and especially ammonium availability (6, 22) could play important roles in the ecological niche differentiation of AOA and AOB. However, only little is known about archaeal versus bacterial ammonia oxidizers in creek or river ecosystems or about the association of AOA with biofilms (37,43). In this study, we used flow channels (FC) as simulated creek ecosystems to investigate the influence of ammonium availability (i) on the establishment of biofilm-associated nitrification activity, (ii) on the community composition of biofilm-associated AOA and AOB, and (iii) on temporal patterns of the abundance of AOA and AOB during a 12-week incubation period.FC experiment. Water for FC was obtained from three shell limestone creeks (Ammerbach, Leutra South, and Leutra North) located near the city of Jena (Thuringia, Germany). The creeks differed in their nutrient concentrations, with the highest nutrient load at Ammerbach resulting from diffuse wastewater inputs (Table 1). Flow velocities ranged from 0.105 Ϯ 0.092 to 0.230 Ϯ 0.224 m s Ϫ1 . Plexiglas FC measuring 160 by 10 by 18 cm (see Fig. S1 in the supplemental material) were set up in triplicate for each creek. Concentrations of NH 4 ϩ in the water phase of the FC were adjusted to 350 mol liter Ϫ1 (Ammerbach; FC 1 to 3), 50 mol liter Ϫ1 (Leutra South, FC 4 to 6), and 20 mol liter Ϫ1 (Leutra North, FC 7 to 9) to run the experiment at three distinct levels of NH 4 ϩ . pHs and concentrat...

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supporting

confidence: 80%

Ammonium Availability Affects the Ratio of Ammonia-Oxidizing Bacteria to Ammonia-Oxidizing Archaea in Simulated Creek Ecosystems

Herrmann

Scheibe

Avrahami

et al. 2011

Appl Environ Microbiol

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“…The films consist of an often complex microbial community composed mainly of bacteria and diatoms (e.g. Teissier & Torre 2002, Lyautey et al 2003, Pei Yuan Quian et al 2003. Later, the larvae also graze on the often more speciesrich biota on water plants.…”

Section: Rearingmentioning

confidence: 99%

Breeding, rearing and raising the red-bellied toad Bombina bombina in the laboratory

Kinne¹,

Kunert²,

Zimmermann³

2004

Endang. Species Res.

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“…They play a marked ecological role in biochemical processes such as organic matter degradation (Romani et al, 2004) or nitrogen biotransformation (Teissier & Torre, 2002).…”

Section: Introductionmentioning

confidence: 99%

The periphyton as a multimetric bioindicator for assessing the impact of land use on rivers: an overview of the Ardières-Morcille experimental watershed (France)

Montuelle¹,

Dorigo²,

Bérard³

et al. 2010

Hydrobiologia

103

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Simultaneous assessment of nitrification and denitrification on freshwater epilithic biofilms by acetylene block method

Cited by 35 publications

References 24 publications

Ammonium Availability Affects the Ratio of Ammonia-Oxidizing Bacteria to Ammonia-Oxidizing Archaea in Simulated Creek Ecosystems

Ammonium Availability Affects the Ratio of Ammonia-Oxidizing Bacteria to Ammonia-Oxidizing Archaea in Simulated Creek Ecosystems

Breeding, rearing and raising the red-bellied toad Bombina bombina in the laboratory

The periphyton as a multimetric bioindicator for assessing the impact of land use on rivers: an overview of the Ardières-Morcille experimental watershed (France)

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