Simultaneous Cellulose Degradation and Electricity Production by
            <i>Enterobacter cloacae</i>
            in a Microbial Fuel Cell

Rezaei, Farzaneh; Xing, Defeng; Wagner, Rachel C.; Regan, John J.; Richard, Tom L.; Logan, Bruce E.

doi:10.1128/aem.02600-08

Cited by 251 publications

(100 citation statements)

References 42 publications

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“…These genera have been described in active sludges from wastewater treatment (Khan, 2002;Sohn et al, 2004;Zhua et al, 2008;Sarma et al, 2010) and polycyclic aromatic hydrocarbon-contaminated soils (Achuthan et al, 2006). E. cloacae has been recently reported as able to produce electricity in an MFC (Rezaei et al, 2009). Therefore, the bacteria identified in the present study are congruent with reports regarding similar samples.…”

Section: Discussionsupporting

confidence: 82%

“…The electrons are transferred to the cathode compartment through an external electric circuit; meanwhile, the protons are transferred to the cathode compartment through the membrane. MFCs provide dual benefits of wastewater treatment and the production of inexpensive and environmentally friendly energy; this technology has been increasing in importance worldwide over the last decade because of its sustainability (Logan et al, 2006;Kumlanghan et al, 2007;Mohan et al, 2009;Rezaei et al, 2009;Ryu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…A microbial fuel cell (MFC) is a bio-system in which microorganisms consume organic substrates such as sugars and metabolize them to produce energy, i.e., transforming chemical energy to electrical energy (Scott and Murano, 2007;Rezaei et al, 2009;Mohan et al, 2009;Venkata et al, 2010). A typical MFC device is composed of 2 compartments, the anode and cathode, which are separated by a proton exchange membrane.…”

Section: Introductionmentioning

confidence: 99%

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Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

Canto-Canché¹,

Tzec-Simá²,

Vázquez-Loría³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) -a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the microbial communities in MFCs. Fast and efficient DNA extraction protocols that are suitable for extracting diverse bacterial genomes are necessary to identify the bacterial diversity present in MFCs and to further monitor the dynamic changes of microbial communities. This study focused on testing different direct cell lysis protocols to extract DNA from a microbial sludge harvested DNA extraction of bacterial consortium in MFC from an MFC. The protocol that achieved the best results was based on a previous study, but was modified by eliminating a chaotropic salt and the special columns used for nucleic acid purification. The efficiency of this less expensive and more straightforward protocol was confirmed by PCR amplification of the 16S rRNA gene and denaturing gradient gel electrophoresis analysis, which confirmed the extraction of multiple genomes. The sequences of 10 clones revealed the presence of phyla, Proteobacteria, Firmicutes and Actinobacteria, comprising both Gramnegative and Gram-positive bacteria. Some of these bacteria were identified at the genus level, e.g., Clostridium, Pseudoxanthomonas, Tistrella, and Enterobacter; these genera have been described in active sludges from wastewater treatment, supporting the congruency of our results. Therefore, this protocol is a useful tool for analysis of the bacteria responsible for energy production in MFCs.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

Canto-Canché¹,

Tzec-Simá²,

Vázquez-Loría³

et al. 2013

Genet. Mol. Res.

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“…The advantage of BESs when using two compartments is that they not solely convert compounds but also separate oxidation and reduction products, which makes it possible to extract useful products out of wastes. Nowadays, BES with bioanodes uses electron donors derived from wastes (e.g., wastewaters; Logan 2005), sediments (Reimers et al 2001), processed energy crops (as cellulose; Niessen et al 2005;Ren et al 2007;Rezaei et al 2009), photosynthetic microorganisms (Strik et al 2008b;Chiao et al 2006;Fu et al 2009), or in situ photosynthesized plant rhizodeposits (Strik et al 2008a;De Schamphelaire et al 2008). BESs, like MFCs treating wastewater, combine energy harvesting with necessary wastewater cleaning.…”

Section: Introductionmentioning

confidence: 99%

New applications and performance of bioelectrochemical systems

Hamelers

Heijne

Sleutels

et al. 2009

Appl Microbiol Biotechnol

257

134

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Bioelectrochemical systems (BESs) are emerging technologies which use microorganisms to catalyze the reactions at the anode and/or cathode. BES research is advancing rapidly, and a whole range of applications using different electron donors and acceptors has already been developed. In this mini review, we focus on technological aspects of the expanding application of BESs. We will analyze the anode and cathode half-reactions in terms of their standard and actual potential and report the overpotentials of these half-reactions by comparing the reported potentials with their theoretical potentials. When combining anodes with cathodes in a BES, new bottlenecks and opportunities arise. For application of BESs, it is crucial to lower the internal energy losses and increase productivity at the same time. Membranes are a crucial element to obtain high efficiencies and pure products but increase the internal resistance of BESs. The comparison between production of fuels and chemicals in BESs and in present production processes should gain more attention in future BES research. By making this comparison, it will become clear if the scope of BESs can and should be further developed into the field of biorefineries.

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“…Table 2 shows the performance of the MFCs using cellulose biomass as substrates. It was found that both cellulolytic and exoelectrogenic microorganisms would be required for electricity generation in BES, because no single strain has yet been capable of producing electricity directly from cellulose [39,40]. An early study reported indirect electricity generation from cellulose in an MFC, through in situ oxidation of hydrogen that was produced from the anaerobic degradation of cellulose by cellulolytic bacteria (Clostridium cellulolyticum and Clostridium thermocellum) [41].…”

Section: Plant Wastementioning

confidence: 99%

Development of Bioelectrochemical Systems to Promote Sustainable Agriculture

Abu-Reesh

2015

Agriculture

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Bioelectrochemical systems (BES) are a newly emerged technology for energy-efficient water and wastewater treatment. Much effort as well as significant progress has been made in advancing this technology towards practical applications treating various types of waste. However, BES application for agriculture has not been well explored. Herein, studies of BES related to agriculture are reviewed and the potential applications of BES for promoting sustainable agriculture are discussed. BES may be applied to treat the waste/wastewater from agricultural production, minimizing contaminants, producing bioenergy, and recovering useful nutrients. BES can also be used to supply irrigation water via desalinating brackish water or producing reclaimed water from wastewater. The energy generated in BES can be used as a power source for wireless sensors monitoring the key parameters for agricultural activities. The importance of BES to sustainable agriculture should be recognized, and future development of this technology should identify proper application niches with technological advancement.

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Simultaneous Cellulose Degradation and Electricity Production by Enterobacter cloacae in a Microbial Fuel Cell

Cited by 251 publications

References 42 publications

Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

New applications and performance of bioelectrochemical systems

Development of Bioelectrochemical Systems to Promote Sustainable Agriculture

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