Simultaneous Characterisation of Fifty Coumarins from the Roots of<i>Angelica dahurica</i>by Off-line Two-dimensional High-performance Liquid Chromatography Coupled with Electrospray Ionisation Tandem Mass Spectrometry

Li, Bin; Zhang, Xu; Wang, Juan; Zhang, Le; Gao, Bowen; Shi, She‐Po; Wang, Xiaohui; Li, Jun; Tu, Pengfei

doi:10.1002/pca.2496

Cited by 67 publications

(58 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compounds were identified using the mass spectra of reference compounds, as well as MS data from the literature (Li et al, 2014;Liu et al, 2011;O'Neill et al, 2013).…”

Section: Hplc-dad Analysis and Hplc-dad-esi-tof-ms Identification Of mentioning

confidence: 99%

Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography

et al. 2015

View full text Add to dashboard Cite

“…Compounds were identified using the mass spectra of reference compounds, as well as MS data from the literature (Li et al, 2014;Liu et al, 2011;O'Neill et al, 2013).…”

Section: Hplc-dad Analysis and Hplc-dad-esi-tof-ms Identification Of mentioning

confidence: 99%

Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography

et al. 2015

View full text Add to dashboard Cite

“…In traditional Chinese medicine, another wildly-grown Angelica species named Angelica dahurica (Baizhi in Chinese) , is used as an herb that purge the body of any negative influences such as heat, clamminess, dryness, and cold on the skin[17]. A number of active constituents such as imperatorin, isoimperatorin, oxypeucedanin, byakangelicol, and byakangelicin have been identified from Angelica Dahurica Radix [18]. Angelica dahurica and its constituents have been reported to possess wide spectrum pharmacological actions.…”

Section: Introductionmentioning

confidence: 99%

Angelica Dahurica ethanolic extract improves impaired wound healing by activating angiogenesis in diabetes

Zhang

Wang

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

Abnormal angiogenesis plays an important role in impaired wound healing and development of chronic wounds in diabetes mellitus. Angelica dahurica radix is a common traditional Chinese medicine with wide spectrum medicinal effects. In this study, we analyzed the potential roles of Angelica dahurica ethanolic extract (ADEE) in correcting impaired angiogenesis and delayed wound healing in diabetes by using streptozotocin-induced diabetic rats. ADEE treatment accelerated diabetic wound healing through inducing angiogenesis and granulation tissue formation. The angiogenic property of ADEE was subsequently verified ex vivo using aortic ring assays. Furthermore, we investigated the in vitro angiogenic activity of ADEE and its underlying mechanisms using human umbilical vein endothelial cells. ADEE treatment induced HUVECs proliferation, migration, and tube formation, which are typical phenomena of angiogenesis, in dose-dependent manners. These effects were associated with activation of angiogenic signal modulators, including extracellular signal-regulated kinase 1/2 (ERK1/2), Akt, endothelial nitric oxide synthase (eNOS) as well as increased NO production, and independent of affecting VEGF expression. ADEE-induced angiogenic events were inhibited by the MEK inhibitor PD98059, the PI3K inhibitor Wortmannin, and the eNOS inhibitor L-NAME. Our findings highlight an angiogenic role of ADEE and its ability to protect against impaired wound healing, which may be developed as a promising therapy for impaired angiogenesis and delayed wound healing in diabetes.

show abstract

“…2 B2), indicating that several constituents in the extract had a good potential for passive absorption. These moderate to high permeant compounds were identified as furanocoumarins by comparing their retention times, UV-DAD, and mass spectra with standards analysed under the same conditions as well as by relating to the MS data from the literature [45][46][47]. Their effective passive permeability values were simply calculated by integrating their resolved UV peaks in the extracts without the need for information on their concentrations (Table S2, Supporting Information).…”

mentioning

confidence: 99%

Prediction of the Passive Intestinal Absorption of Medicinal Plant Extract Constituents with the Parallel Artificial Membrane Permeability Assay (PAMPA)

et al. 2016

View full text Add to dashboard Cite

At the early drug discovery stage, the high-throughput parallel artificial membrane permeability assay is one of the most frequently used in vitro models to predict transcellular passive absorption. While thousands of new chemical entities have been screened with the parallel artificial membrane permeability assay, in general, permeation properties of natural products have been scarcely evaluated. In this study, the parallel artificial membrane permeability assay through a hexadecane membrane was used to predict the passive intestinal absorption of a representative set of frequently occurring natural products. Since natural products are usually ingested for medicinal use as components of complex extracts in traditional herbal preparations or as phytopharmaceuticals, the applicability of such an assay to study the constituents directly in medicinal crude plant extracts was further investigated. Three representative crude plant extracts with different natural product compositions were chosen for this study. The first extract was composed of furanocoumarins (Angelica archangelica), the second extract included alkaloids (Waltheria indica), and the third extract contained flavonoid glycosides (Pueraria montana var. lobata). For each medicinal plant, the effective passive permeability values Pe (cm/s) of the main natural products of interest were rapidly calculated thanks to a generic ultrahigh-pressure liquid chromatography-UV detection method and because Pe calculations do not require knowing precisely the concentration of each natural product within the extracts. The original parallel artificial membrane permeability assay through a hexadecane membrane was found to keep its predictive power when applied to constituents directly in crude plant extracts provided that higher quantities of the extract were initially loaded in the assay in order to ensure suitable detection of the individual constituents of the extracts. Such an approach is thus valuable for the high-throughput, cost-effective, and early evaluation of passive intestinal absorption of active principles in medicinal plants. In phytochemical studies, obtaining effective passive permeability values of pharmacologically active natural products is important to predict if natural products showing interesting activities in vitro may have a chance to reach their target in vivo.

show abstract

Simultaneous Characterisation of Fifty Coumarins from the Roots ofAngelica dahuricaby Off-line Two-dimensional High-performance Liquid Chromatography Coupled with Electrospray Ionisation Tandem Mass Spectrometry

Abstract: The method described here allows rapid and convenient identification of the coumarins in A. dahurica, and may be applied to other herbal medicines containing linear furanocoumarins.

Cited by 67 publications

References 39 publications

Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography

Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography

Angelica Dahurica ethanolic extract improves impaired wound healing by activating angiogenesis in diabetes

Prediction of the Passive Intestinal Absorption of Medicinal Plant Extract Constituents with the Parallel Artificial Membrane Permeability Assay (PAMPA)

Contact Info

Product

Resources

About