Simultaneous conversion of all cell wall components by an oleaginous fungus without chemi-physical pretreatment

Xie, Shangxian; Qin, Xing; Cheng, Yanbing; Laskar, Dhrubojyoti D.; Qiao, Weichuan; Sun, Su; Reyes, Luis H.; Wang, Xin; Dai, Susie Y.; Sattler, Scott E.; Kao, Katy C.; Yang, Bin; Zhang, Xiaoyu; Yuan, Joshua S.

doi:10.1039/c4gc01529k

Cited by 56 publications

(29 citation statements)

References 50 publications

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“…Besides MnP, versatile peroxidases were described as a new family of ligninolytic peroxidases, which had a hybrid molecular architecture that combined different oxidation sites with the heme cofactor for the oxidation of some high redox potential aromatic substrates [25]. Previous reports suggested that peroxidase synergized the redox condition and the Fenton reaction to maximize aromatic polymer degradation [5,8,9]. This study indicated a similar mechanism with specific refined enzymes.…”

Section: Overview Of Differential Gene Expressionmentioning

confidence: 56%

Genomic and molecular mechanisms for efficient biodegradation of aromatic dye

Sun

Xie

Chen

et al. 2016

Journal of Hazardous Materials

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Understanding the molecular mechanisms for aromatic compound degradation is crucial for the development of effective bioremediation strategies. We report the discovery of a novel phenomenon for improved degradation of Direct Red 5B azo dye by Irpex lacteus CD2 with lignin as a co-substrate. Transcriptomics analysis was performed to elucidate the molecular mechanisms of aromatic degradation in white rot fungus by comparing dye, lignin, and dye/lignin combined treatments. A full spectrum of lignin degradation peroxidases, oxidases, radical producing enzymes, and other relevant components were up-regulated under DR5B and lignin treatments. Lignin induced genes complemented the DR5B induced genes to provide essential enzymes and redox conditions for aromatic compound degradation. The transcriptomics analysis was further verified by manganese peroxidase (MnP) protein over-expression, as revealed by proteomics, dye decolorization assay by purified MnP and increased hydroxyl radical levels, as indicated by an iron reducing activity assay. Overall, the molecular and genomic mechanisms indicated that effective aromatic polymer degradation requires synergistic enzymes and radical-mediated oxidative reactions to form an effective network of chemical processes. This study will help to guide the development of effective bioremediation and biomass degradation strategies.

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Section: Overview Of Differential Gene Expressionmentioning

confidence: 56%

Genomic and molecular mechanisms for efficient biodegradation of aromatic dye

Sun

Xie

Chen

et al. 2016

Journal of Hazardous Materials

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“…The total lipids of S. cerevisiae were extracted in the form of fatty acid methyl ester (FAME) using the sulfuric acid-methanol method as previously described [34], details can be found in Supplementary method. The lipid composition was analyzed by GC-MS using a SHIMADZU-QP2010 SE GC-MS (SHIMADZU CORPORATION, Japan) equipped with a ZB-5MSi column (thickness 0.25 µm; length 30 m; diameter 0.25 mm) as previously described [34].…”

Section: Lipid Quantificationmentioning

confidence: 99%

Effects of Seawater on Carotenoid Production and Lipid Content of Engineered Saccharomyces cerevisiae

et al. 2019

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The use of seawater in fermentation can potentially reduce the freshwater burden in the bio-based production of chemicals and fuels. We previously developed a Saccharomyces cerevisiae carotenoids hyperproducer SM14 capable of accumulating 18 mg g−1 DCW (DCW: dry cell weight) of β-carotene in rich media (YPD). In this work, the impacts of seawater on the carotenoid production of SM14 were investigated. When using nutrient-reduced media (0.1× YNB) in freshwater the β-carotene production of SM14 was 6.51 ± 0.37 mg g−1 DCW; however in synthetic seawater, the production was increased to 8.67 ± 0.62 mg g−1 DCW. We found that this improvement was partially due to the NaCl present in the synthetic seawater, since supplementation of 0.5 M NaCl in freshwater increased β-carotene production to 11.85 ± 0.77 mg g−1 DCW. The combination of synthetic seawater with higher carbon-to-nitrogen ratio (C:N = 50) further improved the β-carotene production to 10.44 ± 0.35 mg g−1 DCW. We further showed that the carotenoid production improvement in these conditions is related with lipid content and composition. These results demonstrated the benefit of using seawater to improve the production of carotenoids in S. cerevisiae, and have the potential to expand the utilization of seawater.

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“…sorghum and maize), brown midrib ( bmr ) mutants have been useful for reducing lignin content (Cherney et al ., ) and altering secondary cell wall composition (Palmer et al ., ), which has led to enhanced saccharification and fermentation efficiencies and improvements in ethanol yields from lignocellulosic biomass (Dien et al ., ) and in vitro digestibility (Aydin et al ., ; Oliver et al ., ). Sorghum bmr mutants have also been shown to increase yields in emerging biofuel fermentation systems that utilize lignin as a substrate for lipid synthesis (Xie et al ., ). Together this research illustrates the plasticity of plants to tolerate changes in lignin composition and the utility of modifying biomass composition for industrial purposes.…”

Section: Introductionmentioning

confidence: 97%

Overexpression of SbMyb60 impacts phenylpropanoid biosynthesis and alters secondary cell wall composition in Sorghum bicolor

Scully

Gries²,

Sarath³

et al. 2016

The Plant Journal

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SUMMARYThe phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. The global regulators of phenylpropanoid metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall composition and the levels of several other aromatic compounds, including anthocyanins and flavonoids. While transcription factors correlated with downregulation of the phenylpropanoid biosynthesis pathway have been identified in several grass species, few transcription factors linked to activation of this pathway have been identified in C4 grasses, some of which are being developed as dedicated bioenergy feedstocks. In this study we investigated the role of SbMyb60 in lignin biosynthesis in sorghum (Sorghum bicolor), which is a drought-tolerant, high-yielding biomass crop. Ectopic expression of this transcription factor in sorghum was associated with higher expression levels of genes involved in monolignol biosynthesis, and led to higher abundances of syringyl lignin, significant compositional changes to the lignin polymer and increased lignin concentration in biomass. Moreover, transgenic plants constitutively overexpressing SbMyb60 also displayed ectopic lignification in leaf midribs and elevated concentrations of soluble phenolic compounds in biomass. Results indicate that overexpression of SbMyb60 is associated with activation of monolignol biosynthesis in sorghum. SbMyb60 represents a target for modification of plant cell wall composition, with the potential to improve biomass for renewable uses.

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Simultaneous conversion of all cell wall components by an oleaginous fungus without chemi-physical pretreatment

Abstract: C. echinulata FR3, a non-basidiomycete oleaginous fungus with strong lignin degradation machinery, could convert all cell wall components to lipid without chemi-physical pretreatment.

Cited by 56 publications

References 50 publications

Genomic and molecular mechanisms for efficient biodegradation of aromatic dye

Genomic and molecular mechanisms for efficient biodegradation of aromatic dye

Effects of Seawater on Carotenoid Production and Lipid Content of Engineered Saccharomyces cerevisiae

Overexpression of SbMyb60 impacts phenylpropanoid biosynthesis and alters secondary cell wall composition in Sorghum bicolor

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