Simultaneous degradation of aflatoxin B1 and zearalenone by Porin and Peroxiredoxin enzymes cloned from Acinetobacter nosocomialis Y1

Solvent Regulation Induced Cathode Aggregation-Induced Electrochemiluminescence of Tetraphenylethylene Nanoaggregates for Ultrasensitive Zearalenone Analysis

“…Zearalenone (ZEN) is a metabolite produced by fusarium. It is a common pollutant in cereals such as corn, wheat, oats, etc. − Meanwhile, ZEN has hepatotoxicity, immunotoxicity, teratogenicity, and carcinogenicity, posing a great threat to human health. ,, The International Agency for Research on Cancer classified it as a Group III carcinogen. , There are several methods available for ZEN detection, such as high-performance liquid chromatography, gas chromatography–mass spectrometry, ultra-performance liquid chromatography/tandem mass spectrometry, , and enzyme-linked immunosorbent assay. , But these methods require expensive equipment, skilled operators, and so on . Consequently, it is urgently necessary to develop a simple operation, low-cost, and easy-to-control approach for ZEN detection.…”

Identification and Modification of Enzymatic Substrate Specificity through Residue Alteration in the Cap Domain: A Thermostable Zearalenone Lactonase

“…Apart from microorganisms, enzymes have also proven to be efficient and eco-friendly biocatalysts for ZEN decontamination. , Lactonases play a crucial role in the feed industry for ZEN decontamination. , One such enzyme, lactonase ZHD101 from Clonostachys rosea IFO 7063, has shown remarkable capability in degrading ZEN . Additionally, lactonases from Fonsecaea monophora, Exophiala spinifera, and Rhinocladiella mackenziei have demonstrated the ability to degrade ZEN.…”

“…Furthermore, Bacillus subtilis Y816 was identified as a strain capable of converting ZEN into ZEN-14-phosphate within a fermentation period of 7 h. 10 Apart from microorganisms, enzymes have also proven to be efficient and eco-friendly biocatalysts for ZEN decontamination. 11,12 Lactonases play a crucial role in the feed industry for ZEN decontamination. 13,14 One such enzyme, lactonase ZHD101 from Clonostachys rosea IFO 7063, has shown remarkable capability in degrading ZEN.…”

Improvement of aflatoxin B1 degradation ability by Bacillus licheniformis CotA-laccase Q441A mutant

“…Among these four kinds of aflatoxins, AFB 1 receives particular attention because of the severe carcinogenicity and immunotoxicity to humans and animals [ 5 ]. Different approaches have been used to remove AFB 1 from grains, such as ammoniation, irradiation, adsorption, ozonation and peroxidation [ 6 ]. However, these methods are expensive and cause adverse effects on nutritional value of cereals [ 6 ].…”

“…Different approaches have been used to remove AFB 1 from grains, such as ammoniation, irradiation, adsorption, ozonation and peroxidation [ 6 ]. However, these methods are expensive and cause adverse effects on nutritional value of cereals [ 6 ]. Because of the depressed performance of traditional physical and chemical approaches to remove AFB 1 , biological methods have shown great potential in AFB 1 degradation [ 7 ].…”