Simultaneous detection of A and B trichothecenes by gas chromatography with flame ionization or mass selective detection

Qualitative and Quantitative Analysis of Mycotoxins

“…There are some reports of validated methods for the analysis of mycotoxins, such as DON, T2 toxin and HT‐2 toxin in cereals by gas chromatographic (GC) methods coupled with flame ionization detector (FID) and mass spectrometry (MS) (Eskola and others 2001; Krska and others 2001; Eke and others 2004; Melchert and Pabel 2004). An European Committee (EC)‐funded project within the framework of the SMT‐programme was organized by Pettersson and Langseth (2002), in which gas chromatographic methods for nivalenol, DON, HT‐2, and T‐2 toxin were assessed.…”

“…Limit of detection of the method was from 0.30 to 0.47 mg/kg for GC‐FID and from 0.05 to 0.35 mg/kg for GC–MSD. Using this cartridge, no further sample cleanup steps are required thus making the developed method time‐ and cost‐effective (Eke and others 2004). Recently, 13 trichothecenes were determined in whole beans, roasted soy, nuts, flour and flakes, textured soy protein, tofu, infant formulas, and fermented products (soy sauce) by gas chromatography/mass spectrometry, and HPLC with fluorescence and UV‐detection (Schollenberger and others 2007).…”

“…During the last decades, several reviews have been written in this area. There are some reports of successful application of HPLC techniques for the analysis of mycotoxins in grains (Li and others 2001, 2006; Razzazi‐Fazeli and others 2002, 2003; Eke and others 2004; Gobel and Lusky 2004; Klotzel and others 2005; Visconti and others 2005), fungal cultures (Delmulle and others 2006), cheese (Kokkonen and others 2005), milk (Sorensen and Elbaek 2005), bee pollen (Garcia‐Villanova and others 2004), cereal products (Aresta and others 2003; Chan and others 2004; Biselli and Hummert 2005), beer and wine (Soleas and others 2001), and feeds (Vrabcheva and others 2000; Biselli and Hummert 2005; Martins and others 2007; Krska and others 2007). These HPLC methods differed significantly in the choice of normal‐phase or reversed‐phase columns of different types, elution mixtures and gradients, detection methods, and sample preparation and purification procedures.…”

Comparision and evaluation of two pretreatments of different mechanisms for multi-Fusarium toxins

“…It is essential to have available precise and reliable analytical methods applicable at the regulatory levels for the residue of the four Fusarium toxins. The main analytical methods for the determination of Fusarium toxins in Grain products are thin layer chromatography (TLC), gas chromatography (GC) [9] , gas chromatography-mass spectrometry (GC-MS) [10] , high performance liquid chromatography (HPLC) [11][12] and liquid chromatography mass spectrometry (LC-MS/MS) [13][14] . These methods are mainly aimed at the rapid screening, qualitative or quantitative detection of a certain type of toxin or a class of structurally similar toxins [15][16] .…”

Development of a Simultaneous Lateral Flow Strip Test for the Rapid and Simple Detection of Deoxynivalenol and Zearalenone

“…Analyses of DON and ZEA have been usually performed by gas chromatography (GC) (Schwadorf and Muller ; Eke and others ), gas chromatography‐mass spectrometry (GC‐MS) (Tanaka and others ; Zhao and others ), high performance liquid chromatography (HPLC) (Saeger and others ; Omurtag and Beyoğlu ) and immunoassays (Kuzdraliński and others ). However, chromatographic methods are unsuitable for the routine screening of large number of samples because GC and HPLC require a complicated clean‐up, enrichment step prior to determination, sophisticated technical equipment, and highly skilled personnel (Kolosova and others ).…”