Simultaneous detection of Aspergillus nidulans, Aspergillus luchuensis and Lichtheimia sp. in a bovine abortion

Pesca, Cristina; Cruciani, Deborah; Agostini, L.; Rossi, E.; Pavone, Silvia; Stazi, Marica; Mangili, Piermario; Crotti, Silvia

doi:10.1016/j.mycmed.2019.100923

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…Thanks to the scientific progress, we now have a better understanding of how specific mycoses affect livestock welfare and production. Some mycoses, such as those causing mycotic abortions and mastitis, have been extensively studied [ 57 , 58 , 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Fungi’s Swiss Army Knife: Pleiotropic Effect of Melanin in Fungal Pathogenesis during Cattle Mycosis

Romero,

Kalinhoff,

Saa

et al. 2023

JoF

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Fungal threats to public health, food security, and biodiversity have escalated, with a significant rise in mycosis cases globally. Around 300 million people suffer from severe fungal diseases annually, while one-third of food crops are decimated by fungi. Vertebrate, including livestock, are also affected. Our limited understanding of fungal virulence mechanisms hampers our ability to prevent and treat cattle mycoses. Here we aim to bridge knowledge gaps in fungal virulence factors and the role of melanin in evading bovine immune responses. We investigate mycosis in bovines employing a PRISMA-based methodology, bioinformatics, and data mining techniques. Our analysis identified 107 fungal species causing mycoses, primarily within the Ascomycota division. Candida, Aspergillus, Malassezia, and Trichophyton were the most prevalent genera. Of these pathogens, 25% produce melanin. Further research is required to explore the involvement of melanin and develop intervention strategies. While the literature on melanin-mediated fungal evasion mechanisms in cattle is lacking, we successfully evaluated the transferability of immunological mechanisms from other model mammals through homology. Bioinformatics enables knowledge transfer and enhances our understanding of mycosis in cattle. This synthesis fills critical information gaps and paves the way for proposing biotechnological strategies to mitigate the impact of mycoses in cattle.

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Section: Discussionmentioning

confidence: 99%

Fungi’s Swiss Army Knife: Pleiotropic Effect of Melanin in Fungal Pathogenesis during Cattle Mycosis

Romero,

Kalinhoff,

Saa

et al. 2023

JoF

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“…The causes of abortions include stress (Garcia‐Ispierto & López‐Gatius, 2019; Roth, 2020), nutritional disorders (Akar & Yildiz, 2005), and infectious pathogens such as fungi, viruses, protozoans, and bacteria (Austin, 2021; Barkallah et al., 2014; Leaver & Hart, 1960; Pesca et al., 2020). Abortions due to brucellosis led to the decline of milk production in 1952 with losses amounting to USD 400 million in the United States (Acha & Szyfres, 2003) and negatively affected the livelihood of small farmers in Sub‐Saharan Africa (McDermott et al., 2013).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

Ntivuguruzwa

Kolo

Mwikarago³

et al. 2022

Veterinary Medicine & Sci

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Background Abortions cause tremendous economic losses in food‐producing animals and may lead to food insecurity. Objectives This study aimed to characterize Brucella spp. and other abortigenic pathogens from aborted tissues of cattle. Methods For cattle, aborted tissues (n = 19) were cultured, and Brucella spp. were detected using the genus‐specific 16S‐23S ribosomal DNA interspacer region (ITS) assay and speciated using Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce‐ladder PCR assays. Brucella negative samples were screened using the eight abortigenic pathogens PCR panel. Samples from an abortion outbreak that occurred within a goat tribe were included in this investigation. Sera of females (n = 8) and males (n = 2) were analyzed using the Rose Bengal Test (RBT) and indirect enzyme‐linked immunosorbent assay (i‐ELISA), while vaginal swabs (n = 3) and aborted tissues (n = 1) were cultured and characterized. Results The ITS‐PCR detected Brucella DNA in cultures from two aborted tissues of cattle (10.5%, [2/19]), which were identified as B. melitensis (n = 1), and B. abortus (n = 1) using AMOS and Bruce‐ladder PCR assays. Campylobacter fetus (n = 7) and Leptospira spp. (n = 4) including co‐infections (n = 2) of C. fetus and Leptospira spp. were identified from the Brucella negative samples of cattle. Goats (100.0%, 10/10) were brucellosis seropositive on RBT and i‐ELISA. Mixed infections caused by B. melitensis and B. abortus were isolated from the vaginal swabs (n = 3) and aborted tissues (n = 1). Discussion and conclusions This is the first identification of abortion‐associated pathogens in aborted cattle indicating the enormous financial losses and a threat to public health. It is therefore essential to include these identified pathogens in the surveillance scheme of veterinary and human services.

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“…and infectious pathogens such as fungi, viruses, protozoans, and bacteria (Austin, 2021;Barkallah et al, 2014;Leaver & Hart, 1960;Pesca et al, 2020). Abortions due to brucellosis led to the decline of milk production in 1952 with losses amounting to USD 400 million in the United States (Acha & Szyfres, 2003) and negatively affected the livelihood of small farmers in Sub-Saharan Africa (McDermott et al, 2013).…”

Section: Brucellosis Of Goat's Tribe With Storm Abortionmentioning

confidence: 99%

Characterization of Brucella spp., and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

Ntivuguruzwa,

Kolo,

Mwikarago

et al. 2024

Preprint

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This study aimed to characterize Brucella spp., and other abortigenic pathogens from aborted tissues of cattle from five selected districts of Rwanda. For cattle, aborted tissues (n=19) were collected, cultured, and Brucella spp. were detected using genus-specific 16S-23S ribosomal DNA interspacer region (ITS) assay and further speciated using AMOS and Bruce-ladder PCR assays. Brucella negative samples were screened using eight abortion pathogens PCR panel ( Anaplasma phagocytophilum, Bovine Herpesvirus 4, Campylobacter fetus, Chlamydophila spp., Coxiella burnetti, Leptospira spp., Listeria monocytogenes, and Salmonella spp.). samples from an abortion outbreak that occurred within a goat tribe in the Nyagatare district were included in this investigation. Sera of females (n=8), and males (n=2) were analysed using the Rose Bengal Test (RBT), and indirect enzyme-linked immunosorbent assay (i-ELISA), while vaginal swabs (n=3), and aborted tissues (n=1) were cultured and characterized. The ITS-PCR detected Brucella DNA in cultures from two aborted tissues of cattle [10.5%, (2/19)] which were identified as B. melitensis (n=1), and B. abortus (n=1) using AMOS and Bruce-ladder PCR assays. Campylobacter fetus (n=7) and Leptospira spp. (n=4) including co-infections (n=2) of C. fetus and Leptospira spp. were identified with the PCR panel from the Brucella negative samples of cattle. Goats (100.0%, 10/10) were brucellosis seropositive on RBT and i-ELISA. Mixed infections caused by B. melitensis and B. abortus were isolated from the vaginal swabs (n=3) and aborted tissues (n=1). The isolation of both B. abortus and B. melitensis indicated cross-infections and mixed livestock farming in Rwanda. This is the first identification of abortion-associated pathogens ( B. abortus, B. melitensis, C. fetus, and Leptospira spp.) in aborted cattle samples in Rwanda indicating the enormous financial losses to cattle owners and a threat to public health. It is therefore essential to include these identified pathogens in the surveillance scheme of veterinary and human services, and raise the awareness of caretakers, abattoir workers, and laboratory personnel.

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Simultaneous detection of Aspergillus nidulans, Aspergillus luchuensis and Lichtheimia sp. in a bovine abortion

Cited by 5 publications

References 16 publications

Fungi’s Swiss Army Knife: Pleiotropic Effect of Melanin in Fungal Pathogenesis during Cattle Mycosis

Fungi’s Swiss Army Knife: Pleiotropic Effect of Melanin in Fungal Pathogenesis during Cattle Mycosis

Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

Characterization of Brucella spp., and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

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