Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

Sáez-Llorens, Xavier; Guzman-Verduzco, L M; Shelton, Sharon; Nelson, John D.; Kupersztoch, Yankel M.

doi:10.1128/jcm.27.7.1684-1688.1989

Cited by 9 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, STs are poorly antigenic and their activities are detected by fluid accumulation in calf or pig intestinal loops or in infant mice (81,308,321). Recently, DNA probes, DNA colony hybridization techniques, and EIA have been introduced for the detection of LT, STa, and STb (199,200,202,234,292,309,322). Serologic Types of ETEC Serologic typing of ETEC is based on the antigenic characteristics of the three major surface antigens (270,272).…”

Section: Detection Of Enterotoxinsmentioning

confidence: 99%

Some infectious causes of diarrhea in young farm animals.

Holland

1990

Clinical Microbiology Reviews

View full text Add to dashboard Cite

Section: Detection Of Enterotoxinsmentioning

confidence: 99%

Some infectious causes of diarrhea in young farm animals.

Holland

1990

Clinical Microbiology Reviews

View full text Add to dashboard Cite

Chemisch modifizierte Oligonucleotide als Sonden und Agentien

Englisch

Gauss

1991

Angewandte Chemie

View full text Add to dashboard Cite

show abstract

Chemically Modified Oligonucleotides as Probes and Inhibitors

Englisch

Gauss

1991

Angew. Chem. Int. Ed. Engl.

158

View full text Add to dashboard Cite

Oligonucleotides bind specifically to single‐stranded nucleic acids to form a double helix if there is a complementary antiparallel nucleotide sequence. In addition, certain oligonucleotides bind specifically to a variety of proteins. Therefore, biological processes involving these nucleic acids or proteins can be modulated (normally inhibited) by addition of the respective oligonucleotides. The effects of these oligonucleotides and the fields of potential application can be broadened by the introduction of chemically modified nucleotides. For instance, replacing oligonucleotide phosphate groups by methylphosphonates results in the loss of one negative charge per nucleotide and the oligonucleotide becomes more lipophilic. An oligonucleotide carrying a reactive group can modify its binding partner. An oligonucleotide covalently linked to a dye can be localized in a biological specimen. Oligonucleotides attached to an enzyme can be detected in very small amounts since the enzyme can catalyze the formation of large amounts of the substance assayed (e.g., a fluorescent product). An important biochemical application is the detection and localization of a messenger RNA or its gene. Medical applications include the detection of bacterial or viral sequences. There is also great interest in inhibiting the translation of messenger RNA and the transcription and replication of DNA. So‐called antisense oligonucleotides are currently being used for the inhibition of protein biosynthesis and of reverse transcription of retroviruses.

show abstract

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

Cited by 9 publications

References 31 publications

Some infectious causes of diarrhea in young farm animals.

Some infectious causes of diarrhea in young farm animals.

Chemisch modifizierte Oligonucleotide als Sonden und Agentien

Chemically Modified Oligonucleotides as Probes and Inhibitors

Contact Info

Product

Resources

About