Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR

Germini, Andrea; Masola, Annalisa; Carnevali, Paola; Marchelli, Rosangela

doi:10.1016/j.foodcont.2008.09.010

Cited by 112 publications

(87 citation statements)

References 12 publications

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“…High PCR amplification yield of the internal control gene (16S rRNA) confirmed the efficiency of the above DNA extraction protocol and the presence of bacteria in the entire tested cheese samples. This finding was correlated with results previously reported by Germini et al, (2009) and in accordance with the general guideline for PCR technique proposed by the European Standardization Committee and International Standard Organization (Anonymous, 2002). Also the efficiency of Up-M-PCR in multiple pathogen detection was evaluated using three standard pathogenic bacteria: S. enterica, L. monocytogenes, E. coli O157:H7 and Nonpathogenic strain of E. coli (BL21).…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Molecular characterization of some food borne pathogens in soft cheese samples collected from Jeddah, Saudi Arabia

Tork¹,

Al-Fattani²,

Al-Kahtani³

et al. 2017

IOSRJPBS

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Section: Discussionsupporting

confidence: 92%

“…Particular primers, ESC-F/R, LIS-F/R, and SAL -F/R described by Kawasaki et al, 2005, Germini et al, 2009, Rahn et al 1992, were taken to identify the presence E. coli O157:H7, L. monocytogenes, and S. enteric, respectively. The primer sets selected for the multiplex PCR assay are revealed in Table 2.…”

Section: Pcr Settingmentioning

confidence: 99%

Molecular characterization of some food borne pathogens in soft cheese samples collected from Jeddah, Saudi Arabia

Tork¹,

Al-Fattani²,

Al-Kahtani³

et al. 2017

IOSRJPBS

View full text Add to dashboard Cite

“…After vortexing of the samples for 10 s and following incubation at 100°C for 8 min, the samples were then centrifuged at 8700 g for 2 min. Pellet was removed, and 2 μL of this solution were used directly in PCR [9].…”

Section: Dna Extraction Methodsmentioning

confidence: 99%

“…Primer sets were developed based on the evaluation and combination of published primer sets. Developed protocol involved an overnight enrichment step followed by DNA isolation and mPCR, and detection limit was as low as 10 cells/25 g in liquid whole egg samples [9].…”

Section: Concurrent Detection Of Foodborne Pathogensmentioning

confidence: 99%

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Evrendilek¹,

Richter²

2017

J Food Microbiol Saf Hyg

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Rapid and specific identification of foodborne pathogens is important for not only for food producers but also consumers as well as food safety authorities. Foods can harbor pathogen bacteria of different species, thus it is also important to be able to detect different pathogens simultaneously from the food samples even though they are in very low number. Detection of bacteria depends on different factors such as the initial number, food matrix, sensitivity of the method, competitor microflora and physical state of the bacteria whether they are injured or not. Simultaneous detection of different bacteria yet adds another hurdle for the level of detection, and thus, different approaches have developed and tested so far to optimize the maximum recovery.

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“…The advantages of using mPCR are that the method is sensitive, saves time and effort as well as laboratory cost (Settanni and Corsetti, 2007;Chen et al, 2012). There have been many studies done in the last decade in successfully developing mPCR procedures for simultaneous detection of three different foodborne pathogens (Park et al, 2006;Germini et al, 2009;AlJobori et al, 2016;Nguyen et al, 2016;Trimoulinard et al, 2017). mPCR is a very useful tool which has been used by other researchers to detect five different foodborne pathogens (Chen et al, 2012), distinguish between five closely-related Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous multiplex Polymerase Chain Reaction detection of Salmonella spp., Escherichia coli O157, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter spp.

Ling¹,

Noramirah²,

Abidatul³

et al. 2018

Food Res.

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Foodborne illness is a global burden that impacts a country politically, economically and socio-economically. The severity of the burden can be unmeasurable as foodborne illness is often an underestimated problem. In order to enlighten the burden, appropriate food safety control measures should be taken. This study aimed to optimize a multiplex Polymerase Chain Reaction (mPCR) detection method to identify foodborne pathogens simultaneously. Six foodborne pathogens namely, Salmonella spp., Escherichia coli O157, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter spp., were targeted in the mPCR detection method. Each mPCR parameter was tested and the outcome was analysed to obtain a successful mPCR protocol to detect the targeted foodborne pathogens. The amplified PCR products showed that the optimized mPCR protocol will be a potential rapid diagnostic tool in foodborne pathogen detection.

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Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR

Cited by 112 publications

References 12 publications

Molecular characterization of some food borne pathogens in soft cheese samples collected from Jeddah, Saudi Arabia

Molecular characterization of some food borne pathogens in soft cheese samples collected from Jeddah, Saudi Arabia

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Simultaneous multiplex Polymerase Chain Reaction detection of Salmonella spp., Escherichia coli O157, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter spp.

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