Simultaneous Detection of Flavonoids, Phenolic Acids and Alkaloids in Abri Herba and Abri Mollis Herba using Liquid Chromatography Tandem Mass Spectrometry

Yan, Wenying; Han, Qingjie; Guo, Panpan; Wang, Chunying; Zhang, Zijian

doi:10.1002/pca.2598

Cited by 22 publications

(17 citation statements)

References 6 publications

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“…By using HPLC–MS/MS, Yan et al could characterize 20 compounds, such as flavonoids, phenolic acids, and alkaloids, included luteolin, in abri herba and abri mollis herba 52 . Calibration curves were linear in the range of 0.13–4680.00 ng/ml for luteolin, with LOD and LOQ of 0.01 and 0.03 ng/ml for luteolin, respectively.…”

Section: Chromatographic Determinationsmentioning

confidence: 99%

Analytical determinations of luteolin

Tesio¹,

Robledo

2021

BioFactors

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Plants, through the photosynthesis process, produce the substances necessary for all the life cycles of nature, which are called "primary metabolites." Moreover, there are some plants that synthesize, in addition to these, other substances with more specific functions, which are known as "secondary metabolites." It is inside this group that flavonoids are located, whose main function is to protect organisms from damage caused by different oxidizing agents. Luteolin (3,4,5,7-tetrahydroxy-flavone) belongs to the sub-class of flavonoids known as flavones and is one of 10,000 flavonoids currently known,

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Section: Chromatographic Determinationsmentioning

confidence: 99%

Analytical determinations of luteolin

Tesio¹,

Robledo

2021

BioFactors

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“…Recently, UHPLC-ESI-MS/MS and HPLC-Q-TOF-MS method were reported to simultaneous qualitative analysis of flavonoids and alkaloids in both positive and negative ESI. 22,23 In this paper, we first measured the alkaloids, flavonoids, and triterpenes in liquorice and Semen Strychni simultaneously via ESI mode switch in different retention periods, which not only achieved approving chromatograph separation (Figure 1), but also had sufficient sensitivity for analytes.…”

Section: Mass Spectrometrymentioning

confidence: 99%

An LC–MS/MS method for determination of bioactive components of liquorice and Semen Strychni in rat plasma: Application to a pharmacokinetics study

Zhang

Deng

Wang

et al. 2017

Drug Testing and Analysis

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Semen Strychni is known for its treatment of rheumatic arthritis with a low therapeutic index. Liquorice contributes a lot in herb detoxification according to the traditional Chinese medicine theory. A simple, rapid, and sensitive liquid chromatography-mass spectrometric method (LC-MS) was developed and validated for simultaneous determination of main bioactive ingredients in liquorice and Semen Strychni in rat plasma. Using moclobemide and cyproterone acetate as the internal standards, the analytes were pretreated via protein precipitation with methanol. An Ultimate AQ-C18 column (3.0 μm, 3.0 × 100 mm) was employed for chromatographic separation, combining with gradient elution. The mobile phase consisted of 0.07% formic acid and 0.12% ammonium acetate in aqueous phase (A) and acetonitrile in organic phase (B). The elution program was as follows: 0-0.5 min, 20% B; 0.5-1 min, 20-60% B; 1-7 min, 60-85% B; and 7-7.5 min, returned to 20% B, then continued to 12 min. Selected reaction monitoring was performed in both positive and negative ESI. Positive mode was adopted for detection of strychnine, brucine, and moclobemide, while negative mode was used for glycyrrhizic acid, glycyrrhetinic acid, liquiritigenin, isoliquiritigenin, liquiritin, and cyproterone acetate. The method was validated for specificity, linearity, matrix effect, recovery, precision, accuracy, and stability. The results show that this method is sensitive, accurate and robust for biological matrix analysis. Moreover, the proposed method was applied to a pharmacokinetic study in Sprague-Dawley rats for investigating the mechanism of which liquorice detoxifies Semen Strychni.

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“…Actually, Abri mollis Herba is often used as Abri Herba, such as Jigucao pills [9,10] and Jigucao capsules [11] produced by Guangxi Yulin Pharmaceutical Refco Group (China). Although phytochemical studies have revealed that both of the herbs contain various chemical constituents, such as alkaloids, sterol compounds, saponins and phenolic compounds and so on [12][13][14], there was the content difference of each identical active composition between Abri mollis Herba and Abri Herba according to our previous study [15]. Accordingly, the rationality of substitution for each other needs to be assessed by further investigation.…”

Section: Introductionmentioning

confidence: 96%

Simultaneous detection of four flavonoids and two alkaloids in rat plasma by LC–MS/MS and its application to a comparative study of the pharmacokinetics between Abri Herba and Abri mollis Herba extract after oral administration

Liu

Yan

Han

et al. 2019

J of Separation Science

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Abri Herba and Abri mollis Herba both were important members of the Leguminosae family in southwestern China. Abri mollis Herba was often used as Abri Herba due to their proximity, but there are few studies on pharmacokinetics to compare their main identical active compositions. A sensitive and selective high‐performance liquid chromatography with tandem mass spectrometry method in the positive/negative electrospray ionization switching mode was developed and validated for the simultaneous analysis of four flavonoids and two alkaloids in rat plasma. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of methanol and 0.5% acetic acid. The detection of the target compounds was conducted in multiple‐reaction monitoring mode with a hybrid triple quadrupole linear ion trap mass spectrometer equipped with positive/negative ion‐switching electrospray ion source. The differences in pharmacokinetics were discovered, which indicated that the substitution between them is some degree of irrationality. The validated method was successfully applied to pharmacokinetic study of the six components in male rat plasma after oral administration of Abri Herba and Abri mollis Herba extract and the results in the study would provide a useful guide for the clinical application of Abri Herba with those in Abri mollis Herba.

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Simultaneous Detection of Flavonoids, Phenolic Acids and Alkaloids in Abri Herba and Abri Mollis Herba using Liquid Chromatography Tandem Mass Spectrometry

Abstract: HPLC-MS/MS method is sensitive and selective and can be suitable for the reliable quality control of Abri mollis Herba and Abri Herba.

Cited by 22 publications

References 6 publications

Analytical determinations of luteolin

Analytical determinations of luteolin

An LC–MS/MS method for determination of bioactive components of liquorice and Semen Strychni in rat plasma: Application to a pharmacokinetics study

Simultaneous detection of four flavonoids and two alkaloids in rat plasma by LC–MS/MS and its application to a comparative study of the pharmacokinetics between Abri Herba and Abri mollis Herba extract after oral administration

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