Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining

Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water

“…Soil aliquots were drawn as follows: 5 g of soil was suspended in PBS (1:5 w:v) (Sigma), shaken (200 RPM X 30 min) and after 10 min of settling the suspension was used for culture of indicators and pathogens including FIB, total coliform, P. aeruginosa and Staphylococcus aureus as previously described (Benami et al, 2013). Five gram was also used for protozoal detection according to Orlofsky et al (2013). One gram was transferred to 9 mL BPW for S. enterica pre-enrichment.…”

Prevalence of Cryptosporidium oocysts and Giardia cysts in raw and treated sewage sludges

“…[37] Other authors [35] also found high counts of Cryptosporidium oocysts, 70%, using IMS and IFA after a spontaneous sedimentation. In contrast, Orlofsky et al [36] obtained better results avoiding the sedimentation step.…”

Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia

“…Documenting the degree of soil contamination with T. gondii oocysts and G. dudodenalis cysts is fundamental to propose efficacious measures to prevent toxoplasmosis and giardiasis ( Slifko et al, 2000 ; Escotte-Binet et al, 2019 ; Dumètre et al, 2012 ; Lee et al, 2021 ; Paller et al, 2022 ). Molecular assays are currently the most sensitive and effective methods to detect T. gondii and G. dudoenalis in soil ( Orlofsky et al, 2013 ; Shapiro et al, 2019a ; Slifko et al, 2000 ).…”