2022
DOI: 10.3390/agriculture12020254
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Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

Abstract: Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths of the plant epidermis, are suitable for monitoring infection processes of powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On the trichomes, tomato powdery mildew (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced a maximum of four vigorously elongated hyphae per conidium, which stopped growth approximately 12 days after inoculation. Single trichome cells, invaded… Show more

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Cited by 2 publications
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“…Mung bean [206] Tomato powdery mildew RT PCR Tomato [207] Waitea circinata ITS rDNA seq Brassica sp. [208] In general, the listed PCR-based assays are highly sensitive.…”
Section: Methods Species Referencementioning
confidence: 99%
“…Mung bean [206] Tomato powdery mildew RT PCR Tomato [207] Waitea circinata ITS rDNA seq Brassica sp. [208] In general, the listed PCR-based assays are highly sensitive.…”
Section: Methods Species Referencementioning
confidence: 99%