Simultaneous detection of the release of glutamate and nitric oxide from adherently growing cells using an array of glutamate and nitric oxide selective electrodes

Castillo, Jaime; Işık, Sonnur; Blöchl, Andrea; Pereira-Rodrigues, Nazaré; Bedioui, Féthi; Csöregi, E.; Schuhmann, Wolfgang; Oni, Joshua

doi:10.1016/j.bios.2004.08.021

Cited by 42 publications

(40 citation statements)

References 21 publications

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“…A variety of chemistries have been explored for efficient detection of H 2 O 2 generated by GluOx, including direct oxidation on metals 12, [24][25][26][27][28][29] (usually coated with a permselective polymer to block interference), metallicized carbon 30,31 or conducting polymercoated Pt, 32 and catalytic oxidation using horseradish peroxidase coupled to a redox polymer. 6,15,20,33,34 Biosensors in general, and especially those designed for tissue implantation, [35][36][37][38][39] must fulfill the following minimum criteria for reliable monitoring of the target analyte: appropriate size and biocompatibility properties, good sensitivity to the enzyme substrate, effective rejection of electroactive interference, and low sensitivity to changes in pO 2 over the range of substrate and oxygen concentrations relevant to the intended application. We recently reported preliminary data and analysis of the oxygen dependence of implantable Glu biosensors incorporating GluOx in a poly(o-phenylenediamine), PPD, interference-rejecting layer electrosynthesized onto 125-µm-diameter Pt cylinders (Pt C ) and disks (Pt D ).…”

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confidence: 99%

Control of the Oxygen Dependence of an Implantable Polymer/Enzyme Composite Biosensor for Glutamate

et al. 2006

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Biosensors for glutamate (Glu) were fabricated from Teflon-coated Pt wire (cylinders and disks), modified with the enzyme glutamate oxidase (GluOx) and electrosynthesized polymer PPD, poly(o-phenylenediamine). The polymer/enzyme layer was deposited in two configurations: enzyme before polymer (GluOx/PPD) and enzyme after polymer (PPD/GluOx). These four biosensor designs were characterized in terms of response time, limit of detection, Michaelis-Menten parameters for Glu (J max and K M (Glu)), sensitivity to Glu in the linear response region, and dependence on oxygen concentration, K M (O 2 ). Analysis showed that the two polymer/enzyme configurations behaved similarly on both cylinders and disks. Although the two geometries showed different behaviors, these differences could be explained in terms of higher enzyme loading density on the disks; in many analyses, the four designs behaved like a single population with a range of GluOx loading. Enzyme loading was the key to controlling the K M (O 2 ) values of these first generation biosensors. The counterintuitive, and beneficial, behavior that biosensors with higher GluOx loading displayed a lower oxygen dependence was explained in terms of the effects of enzyme loading on the affinity of GluOx for its anionic substrate. Some differences between the properties of surface immobilized GluOx and glucose oxidase are highlighted.L-Glutamate (Glu) is the most widespread excitatory neurotransmitter in the mammalian central nervous system, 1 plays a major role in a broad range of brain functions, and has been implicated in a number of neurological disorders. 2 Indeed, the development of devices for Glu detection has become an important research area due to the value of monitoring this key amino acid in a number of complex matrixes, including food processing, 3,4 cell cultures, 5-7 tissue slices ex vivo, 8,9 and intact brain in vivo. [10][11][12][13][14][15] A number of designs of biosensors for Glu monitoring have been reported and, although Glu receptors 16 Brain Res. Bull. 2002, 58, 401-404. (10) Burmeister, J. J.; Gerhardt, G. A. Trends Anal. Chem. 2003, 22, 498-502. (11)

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confidence: 99%

Control of the Oxygen Dependence of an Implantable Polymer/Enzyme Composite Biosensor for Glutamate

et al. 2006

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“…This setup was extended by simultaneously measuring NO and glutamate levels after bradykinin stimulation, as shown in Figure 2B. In this experimental setup, NO was sensed with a platinized gold electrode coated with a NiTmPyP layer and glutamate was monitored with a gold electrode modified with a horseradish peroxidase/glutamate oxidase hydrogel (Castillo et al , 2005 ).…”

Section: In Vitro Cell Measurementsmentioning

confidence: 99%

“…These experiments have been performed on microelectrode arrays (Figure 2 A) coated with a thick layer of biocompatible gels (Castillo et al , 2005 ;Wartelle et al , 2005 ). Wartelle and coworkers used a carbon electrode modified by electrodepositing a NiTSPc film to measure NO levels after exposure to bradykinin and after NOS inhibition with L-NAME (Wartelle et al , 2005 ).…”

Section: In Vitro Cell Measurementsmentioning

confidence: 99%

Biological applications of the electrochemical sensing of nitric oxide: fundamentals and recent developments

Trouillon

2012

Biological Chemistry

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Nitric oxide (NO) is a unique cellular messenger linked to a number of important biological processes. Its free radical nature, small size and fast diffusivity make it highly reactive and membrane permeable. Unfortunately, its reactivity, coupled with the inherent complexity of in situ biological measurements, makes it a challenge to detect. For the past 20 years, electrochemical methods have been used to investigate the role of NO in a number of biological processes, including vascular physiology, immune response, neuronal mediation, tissue growth and oxidative stress. This review examines the biological applications of electrochemical NO sensors and the technologies used to elucidate different physiological phenomena associated with this unique biomolecule with a specific focus on the developments and innovations reported in the last 3 years.

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“…Many glutamate biosensors are based on quantifying the oxidation of hydrogen peroxide (H 2 O 2 ) liberated in a chemical reaction between glutamate oxidase (GmOx, an oxidoreductase enzyme, EC 1.4.3.11) and L-glutamate in the presence of oxygen (O 2 ), water (H 2 O), and a flavin adenine dinucleotide (FAD) cofactor [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45]. GmOx enzyme catalyzes the oxidative deamination of glutamate resulting in the formation of 2-oxoglutarate (i.e., α-ketoglutarate, α-KG), ammonia (NH 3 ), and H 2 O 2 [46].…”

Section: Glutamate Oxidase-based Biosensorsmentioning

confidence: 99%

Enzyme-Based Electrochemical Glutamate Biosensors

Okon¹,

Ronkainen²

2017

Electrochemical Sensors Technology

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Glutamate, a major excitatory neurotransmitter in the mammalian central nervous system, plays a vital role in many physiological processes and is one of the key neurotransmitters of interest in psychopharmacology. It is involved in many normal and abnormal behaviors related to neurological and psychiatric disorders. The glutamate system has been proposed to play a significant role in various neurological and psychiatric disorders such as Alzheimer's disease, autism, schizophrenia, depression, drug addiction, and more. The design, construction, and optimization of enzyme-based electrochemical biosensors for in vivo and in vitro detection of glutamate are active areas of interdisciplinary research. For example, various glutamate biosensors have been developed for monitoring dynamic levels of extracellular glutamate in the living brain tissue adding to the current medical knowledge of these complex neurotransmitter systems and ultimately impacting treatment plans. In addition to biological sciences and clinical medicine, glutamate biosensors have been used in environmental monitoring, in the fermentation industry, and in the food industry for determination of monosodium glutamate (MSG), a common flavor-enhancing food additive.

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Simultaneous detection of the release of glutamate and nitric oxide from adherently growing cells using an array of glutamate and nitric oxide selective electrodes

Cited by 42 publications

References 21 publications

Control of the Oxygen Dependence of an Implantable Polymer/Enzyme Composite Biosensor for Glutamate

Control of the Oxygen Dependence of an Implantable Polymer/Enzyme Composite Biosensor for Glutamate

Biological applications of the electrochemical sensing of nitric oxide: fundamentals and recent developments

Enzyme-Based Electrochemical Glutamate Biosensors

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