Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants

Rood, Jon J. van; Leeuwen, A. van; Ploem, J. S.

doi:10.1038/262795a0

Cited by 475 publications

(113 citation statements)

References 11 publications

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“…B, and C typing was done by means of a complement-dependent microcytotoxicity assay (21). HLA-DR and HLA-DQ typing was performed using a 2-color fluorescence technique (22). HLA-A.…”

Section: Methodsmentioning

confidence: 99%

Human autoantibodies against the 70‐kd polypeptide of u1 small nuclear rnp are associated with hla‐dr4 among connective tissue disease patients

Hoffman

Rettenmaier

Takeda

et al. 1990

Arthritis & Rheumatism

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“…B, and C typing was done by means of a complement-dependent microcytotoxicity assay (21). HLA-DR and HLA-DQ typing was performed using a 2-color fluorescence technique (22). HLA-A.…”

Section: Methodsmentioning

confidence: 99%

Human autoantibodies against the 70‐kd polypeptide of u1 small nuclear rnp are associated with hla‐dr4 among connective tissue disease patients

Hoffman

Rettenmaier

Takeda

et al. 1990

Arthritis & Rheumatism

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“…RF positivity was defined as >12.50 IU (titer 1:160) by latex fixation and/or >25 IU (titer 1:16) by Rose-Waaler test. One hundred thirty-one of the RA patients (97%) and 357 of the controls (94%) were typed for HLA-DR. HLA-DR typing was done by the double-fluorescence technique described by Van Rood et a1 (27), with a battery of well-characterized antisera defining all recognized DR specificities.…”

Section: Methodsmentioning

confidence: 99%

Reduction of the risk of rheumatoid arthritis among women who take oral contraceptives

Hazes

Dijkmans

Vandenbroucke

et al. 1990

Arthritis & Rheumatism

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Discrepant results among investigations of the association between oral contraceptive use and rheumatoid arthritis (RA) have been ascribed to shortcomings in the study methods. In the present study, we obtained detailed information on oral contraceptive use in 135 young women with confirmed definite or classic RA of recent onset and in 378 controls with soft tissue rheumatism or osteoarthritis. All patients had at least 2 years of followup to confirm the consistency of the diagnosis. Oral contraceptive use at any time before disease onset was reported by 70% of the RA patients and 85% of the controls; the latter figure corresponded to general population data for The Netherlands. This yielded a relative risk for RA of 0.39 among those who had ever used oral contraceptives and 0.58 for those using oral contraceptives at the time of symptom onset. The preventive effect of oral contraceptive use on the risk of RA was found to be independent of the dose, duration of use, or presence of HLA-DR4. The strongest protection was seen in women with a family history of RA and in women ages 31-40 at symptom onset. The conflicting results in the literature are discussed, and a possible biologic mechanism for this phenomenon is suggested.From the

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“…(19). H342 alone distinguishes "live" cells from "dead" ones (21). "Dead" cells fluoresce almost immediately after adding the dye to the cells, while "viable" cells take up the dye gradually, reaching saturation after approximately 30 min.…”

mentioning

confidence: 99%

Permeabilization of lymphocytes with polyethylene glycol 1000. Discrimination of permeabilized cells by flow cytometry

1982

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The toxicity of polyethylene glycol 1000 (PEG) used similarly as in cell hybridization experiments, has been studied by flow cytometry, measuring the light scattering and fluorescence distributions of PEG‐treated human lymphocytes stained with propidium iodide, fluorescein diacetate and acridine orange (AO). The sensitivity of these tests to detect permeabilized, or potentially dead cells, was equal. In addition, PEG proved to interfere with AO staining most likely through the inhibition of its binding to nucleic acids. The decrease of AO fluorescence in cells killed by PEG was unexpected since intercalation of propidium iodide was the same as in alcohol fixed cells. Permeabilization of cells by PEG appears to be an all‐or‐none phenomenon, accompanied by entrance of PEG into the cells. The findings are described in the context of a review of the currently used flow cytometric techniques to discriminate viable and lethally affected cells; also, the problems of interpretation are discussed.

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Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants

Cited by 475 publications

References 11 publications

Human autoantibodies against the 70‐kd polypeptide of u1 small nuclear rnp are associated with hla‐dr4 among connective tissue disease patients

Human autoantibodies against the 70‐kd polypeptide of u1 small nuclear rnp are associated with hla‐dr4 among connective tissue disease patients

Reduction of the risk of rheumatoid arthritis among women who take oral contraceptives

Permeabilization of lymphocytes with polyethylene glycol 1000. Discrimination of permeabilized cells by flow cytometry

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