Simultaneous detection of tylosin and tilmicosin in honey using a novel immunoassay and immunochromatographic strip based on an innovative hapten

Song, Yuehong; Song, Shanshan; Liu, Liqiang; Kuang, Hua; Guo, Lingling

doi:10.1080/09540105.2015.1089843

Cited by 34 publications

(17 citation statements)

References 32 publications

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“…if both of the lines do not appear (Figure 2(C)), the test procedure is incorrect or the strip is invalid, declaring that the test should be repeated with a new strip. (Mukunzi et al, 2016;Song et al, 2015).…”

Section: Ics Assaymentioning

confidence: 99%

Development of immunocolloidal strip for rapid detection of pyrimethanil

Chen

et al. 2019

Food and Agricultural Immunology

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In this study, we prepared a monoclonal antibody against pyrimethanil, for which we first derived its hapten according to the structural formula of pyrimethanil; we used indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to screen positive mice for cell fusion; finally, we developed five cell lines (1A5, 1E7, 3A6, 3H12 and 5D4) that produced monoclonal antibodies to pyrimethanil. The monoclonal antibody produced by each cell was detected by ic-ELISA, and the monoclonal antibody produced by the 1E7 cell strain was found to be the most sensitive to pyrimethanil. The IC50 value of this antibody was 4 ng/ml. After using this monoclonal antibody, i.e. 1E7, to perform immunocolloidal gold strip treatment on cucumber samples, the PBS and the cucumber samples cutoff value of Immunochromatographic strips (ICS) was found to be 50 ng/ml; We conclude that ICS can rapidly detect pyrimethanil in cucumber samples.

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Section: Ics Assaymentioning

confidence: 99%

Development of immunocolloidal strip for rapid detection of pyrimethanil

Chen

et al. 2019

Food and Agricultural Immunology

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“…The mouse with the best affinity and inhibition for OFL was sacrificed to fuse. The procedures of cell fusion were described previously [30]. Briefly, three days before fusion, the selected mouse was boosted with 25 µg immunogen mixed with saline, and SP2/0 myeloma cells were cultured.…”

Section: Production Of Mabmentioning

confidence: 99%

Gold Nanoparticle-Based Paper Sensor for Highly Specific Detection of Ofloxacin in Beef

Oliveira¹,

Soares²,

Teixeira³

et al. 2019

J Food Chem Nanotechnol

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Ofloxacin (OFL), a third-generation fluoroquinolone, is widely used as an antibacterial drug to prevent diseases in livestock and poultry. In this study, a paper sensor based on gold nanoparticle and a highly specific monoclonal antibody (mAb) against OFL was prepared to detect OFL residues in beef. The limit of detection of the developed immunochromatographic strip for OFL reached 0.16 ng/mL, and the linear range was from 3.125 ng/mL to 100 ng/mL, with the following optimal parameters for the preparation of colloidal gold-labeled mAb probe: pH 6.5, 2 mg/mL OFL-bovine serum albumin antigen, 10 µg/mL antibody, and 12 min immunoreaction time. Cross-reactivity (CR) experiment indicated that the developed strip is highly specific and features low CR with fluoroquinolone analogues. Overall, the developed strip is reliable for the rapid detection of OFL in beef and can be considered an effective screening method for food safety and quality management.

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“…The cell culture supernatants were screened with indirect competitive ELISA (ic-ELISA) as described (Liu, Xing, et al, 2014;Song et al, 2016;Zhu, Song, Liu, Kuang, & Xu, 2016). First, 96-well microtitre plates were coated with MD-OVA at the concentration of 0.2 μg/ml in coating buffer (0.05 M carbonate buffer solution) with 100 μl/well at 37°C for 2 h. Second, the plates were washed with PBS with 0.05% Tween-20 three times, then 200 μl blocking buffer (coating buffer with 0.1% gelatin) was added to each well and incubated at 4°C overnight.…”

Section: Screening Of Cell Culture Supernatantsmentioning

confidence: 99%

Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples

Guo

Song

et al. 2017

Food and Agricultural Immunology

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Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 10 10 l/mol, and the isotype was IgG3. The MD-mAb has no cross-reactivity with other polyether antibiotics. Using this MD-mAb, a gold immunochromatographic assay was developed to detect MD residues in chicken breast and egg samples. For semi-quantitative detection by the naked eye, the visual limit of detection was 5 ng/g in chicken breast, 10 ng/g in egg. Quantitative results can be obtained by a hand-held strip scan reader, with the detection range of 5.11-19.34 ng/g in chicken breast and 6.46-27.87 ng/g in egg. The strip test took 10 min to run in total. This strip assay is suitable for on-site detection of MD residues in chicken breast and egg samples. ARTICLE HISTORY

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Simultaneous detection of tylosin and tilmicosin in honey using a novel immunoassay and immunochromatographic strip based on an innovative hapten

Cited by 34 publications

References 32 publications

Development of immunocolloidal strip for rapid detection of pyrimethanil

Development of immunocolloidal strip for rapid detection of pyrimethanil

Gold Nanoparticle-Based Paper Sensor for Highly Specific Detection of Ofloxacin in Beef

Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples

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