Simultaneous determination of 12 active components in the roots ofPulsatilla chinensisusing tissue-smashing extraction with liquid chromatography and mass spectrometry

Abstract

Ultra high performance liquid chromatography coupled with mass spectrometry and combining a tissue-smashing extraction technique was developed for the simultaneous quantitative analysis of 12 compounds in the roots of Pulsatilla chinensis. Among them, compound 6 was characterized and accurately quantified in this herb for the first time. The parameters of extraction condition were simultaneously optimized with a Box-Behnken design and Derringer's function. The optimized conditions were as follows: sample quantity of 0.5 g, ethanol concentration of 70%, and extraction time of 200 s. Multiple-reaction monitoring scanning was employed for the quantification between positive and negative mode in a single run of 6 min. Full validation of the method was carried out, and the results indicated that the method was rapid, specific, and reliable. The developed method was successfully applied to quantify the 12 compounds in 33 batches of P. chinensis from different provinces. Moreover, the principal component analysis was performed to compare the P. chinensis collected from different provinces of China based on quantitative data and the results indicated that the content of compounds could be used to differentiate the origins of P. chinensis. These results demonstrated that this method is feasible and reliable for the quality control of P. chinensis.

Development of a UHPLC–MS/MS method for the quantitation of bioactive compounds in Phyllanthus species and its herbal formulations

“…Compared with the HPLC and HPTLC methods, the UHPLC-ESI-MS/MS method in multiple reaction monitoring (MRM) acquisition mode is more effective in rapidly quantifying trace compounds due to its rapid separation power, low detection limit, better accuracy, high specificity, and resolution [12,[35][36][37][38][39].…”

Identification ofPulsatilla chinensis(Bge.) Regel and look‐alike species by UHPLC‐TOF‐MS using multivariate statistical analysis

“…Modern studies have shown that it has anti‐inflammatory, antibacterial, antioxidant, antitumor, and other pharmacological effects [1–15], leading to its application in proprietary Chinese medicine. Triterpenoid saponins were predominant biologically active constituents, and these were subcategorized into lupane and oleanane saponins on skeletal basis [16, 17]. Nonetheless, various look‐alike species are marketed and sold as P. chinensis (Bge.)…”

“…Modern studies have shown that it has anti-inflammatory, Article Related Abbreviations: EMRT, exact mass retention time; OPLS-DA, orthogonal partial least square discriminant analysis; PCA, principal component analysis; RT, retention time antibacterial, antioxidant, antitumor, and other pharmacological effects [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15], leading to its application in proprietary Chinese medicine. Triterpenoid saponins were predominant biologically active constituents, and these were subcategorized into lupane and oleanane saponins on skeletal basis [16,17]. Nonetheless, various look-alike species are marketed and sold as P. chinensis (Bge.)…”

Characterisation of hederacoside C metabolites using ultrahigh‐performance liquid chromatography quadrupole Orbitrap mass spectrometry based on automatic fragment ion search

“…Hederacoside C (3‐[{2‐ O ‐(α‐ l ‐rhamnopyranosyl)‐α‐ l ‐arabinopyranosyl}oxy]‐23‐hydroxyolean‐12‐en‐28‐oicacid6‐ O ‐{4‐ O ‐(α‐ l ‐rhamnopyranosyl)‐β‐ d ‐glucopyranosyl}‐β‐ d ‐glucopyranosyl ester, HDC) is a chief bioactive natural triterpenoid saponin . It is not only widely found in the Pulsatilla Adans , but also existed in the Hedera helix .…”