Simultaneous determination of 13 bioactive compounds in Herba Artemisiae Scopariae (Yin Chen) from different harvest seasons by HPLC–DAD

Chromatographic separation techniques and data handling methods for herbal fingerprints: A review

“…High performance liquid chromatography (HPLC) is the most popular analytical technique for analyzing herbal products [38,39,[79][80][81][82][83][84][85][86][87][88][89][90][91][92][93]. HPLC is an easy to operate, fully automatable technique with high resolution, selectivity and sensitivity [94,95].…”

“…For herbal fingerprinting, many papers were published using ultra violet (UV) [39,79,86,87,[89][90][91] and diode array detection (DAD) [38,80,82,84,85,88,92] for UV absorbing compounds, evaporative light scattering detection (ELSD) [79,89] and chemiluminescence detection (CL) for non-UV absorbing compounds, nuclear magnetic resonance (NMR) for metabolomic profiling [85], and mass spectrometry (MS) [38,82,83,85,88,93] for identification of the separated compounds. Furthermore, several papers demonstrate the use of HPLC for multi-compound quantization in complex biological samples [84]. However, HPLC requires rather expensive machinery and often uses large volumes of environmentally unfriendly liquids.…”

“…Most commonly used are the Diode Array Detector (DAD) [80,84,172,173] and Mass Spectrometry (MS) [38,82,83,85] collecting the UV spectra or the mass spectra of the eluting compounds, respectively. For multiple fingerprints, the entire sample is subjected to two or more individual and related separation techniques (e.g.…”

Recent Developments in Chromatographic Fingerprints from Herbal Products: Set-Up and Data Analysis

“…Most used are particle-based C18 columns in Reversed-Phase (RP) HPLC [8,9,70,74,75,80,81,85,92]. A monolithic column with similar properties is an alternative.…”

“…Differences are also caused by the treatment of the plants, e.g. the way of drying, washing, crushing and pulverizing, the storage and the conservation [3][4][5][6][7][8][9][10][11][12][13][14]. Moreover different extraction methods will result in different mixtures.…”