Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection

Abstract

Abstract:The paper described a novel chromatographic method for the simultaneous determination of phenolic compounds such as gallic, protocatechuic, vanillic, caffeic, syringic, p-coumaric and salicylic acid, (+)-catechin, (-)-epicatechin, rutin, morin, quercetin, coumarin and trans-resveratrol at their maximum absorbance wavelengths (MAW) employing reverse-phase high performance liquid chromatography combined with DAD and UV detection via detection wavelength switching. The method was based on MAW acquisition by DAD and quantification by UV. The separation process was performed on a Shim-Pack VP-ODS C 18 column (250 mm × 4.6 mm, 5 μm) held at 30 °C, utilizing 3.0% acetic acid and acetonitrile as mobile phase at a flow rate of 0.8 mL/min in the gradient elution mode. The method was fully validated in terms of linearity (r 2 > 0.9990, 10-350 mg/L), precision (both intra-day and inter-day RSD < 4.22%), accuracy (97.31%-104.66%), specificity, robustness (0.59% < RSD < 2.86%), limit of detection and quantification. The switching method significantly improved the sensitivities of most phenolics studied in comparison with the standard constant wavelength detection (280 nm). OPEN ACCESSMolecules 2013, 18 14242The proposed method has been successfully applied to the determination of 14 phenolic compounds in 89 varieties of one-year-old Chinese grape one-year-canes. Grape canes contain many phenolics, especially trans-resveratrol, (-)-epicatechin, and (+)-catechin.

Second‐order capillary electrophoresis diode array detector data modeled with the Tucker3 algorithm: A novel strategy for Argentinean white wine discrimination respect to grape variety

“…Interestingly the profiles represented in Fig. , retrieved by the Tucker3 algorithm, show high agreement with white wine constituents UV‐VIS spectra profiles reported in literature . In particular, the factors 1 and 2 (represented by the blue and black solid lines, respectively) are directly linked to varietal discrimination of Argentinean white wine samples.…”

“…In particular, the factors 1 and 2 (represented by the blue and black solid lines, respectively) are directly linked to varietal discrimination of Argentinean white wine samples. Comparing these two profiles with spectral profiles shown in the literature it is possible to see remarkable similarity with the spectra profiles of the phenolic compounds, rutin and quercetin reported in .…”

Environmental plasticity of Pinot noir grapevine leaves: A trans‐European study of morphological and biochemical changes along a 1,500‐km latitudinal climatic gradient

“…This is largely due to flavonols and especially the dominant quercetin-3-O-glucuronide absorbing in both wavelength regions (Tables 5A and S3A). This result is consistent with the proposed contribution of phenolic compounds in shielding against UV radiation (Barnes et al, 2016;Bidel et al, 2007) and the relatively higher absorption by phenolic acids in the UV-B compared to the UV-A wavelength range (Zhang et al, 2013).…”

Detection of olive oil adulteration by low-field NMR relaxometry and UV-Vis spectroscopy upon mixing olive oil with various edible oils

“…The common maximum absorbance values in the UV-Vis spectra are approximately at 244.0 nm and attributed to elenolic acid, a compound significant in concentration in the phenolic fraction of olive oil, and 282.0 nm to phenolic compounds (tyrosol, hydroxytirosol, and tocopherols) (Fuentes et al, 2012). The oils other than olive oil have a common peak at around 272.0 nm arising from syringic acid, which is a phenolic compound, while the peak around 260.0-265.0 nm and observed in the spectra of Koroneiki, France-Nyons olive oils and in castor, corn, soybean and sunflower seed oils was explained by the presence of phenolic compounds, vanilic acid, and protocatechuic acid (Zhang et al, 2013). Flax seed oil has a unique band around at 320.0 nm, which is linked to the existence of some flavonoids like apigenin and luteolin (LermaGarcia et al, 2009).…”