Simultaneous determination of 17 ginsenosides in rat urine by ultra performance liquid chromatography–mass spectrometry with solid-phase extraction

Abstract

A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided.

Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids: a review

“…Studies which used positive ion mode electrospray for the analysis of ginsenosides indicated that the predominant and almost exclusive ion observed was the [M + Na] + ion (Cui et al 2001, Wang et al, 2007. van Breeman et al (1995) reported that the addition of Na + as NaCl did not improve the sensitivity.…”

“…Another sample preparation procedure for the analysis of ginsenoside and metabolites has been illustrated by a number of authors (Sun et al, 2005;Wang et al, 2007;Yu et al, 2007a, b) and involves solid-phase extraction with vacuum manifolds:…”

“…Today atmospheric pressure ionization interface sources dominate LC-MS hyphenated systems. These include atmospheric pressure chemical ionization (APCI) (Pfeifer et al, 2001), atmospheric glow discharge ionization (Dalton and Glish, 2003), thermospray ionization (Blakley and Vestal, 1983), electrospray ionization (ESI) (Mauri et al, 1999;Hau and Bovetto, 2001;Wang et al, 2007), sonic spray ionization (Dams et al, 2002), cold spray ionization (Yamaguchi, 2003) and atmospheric pressure photoionization (Robb et al, 2000).…”

“…4. Extracts were then either injected (Sun et al, 2005;Wang et al, 2007) or evaporated under a stream of nitrogen and reconstituted in 100 μL of mobile phase (Yu et al, 2007a, b).…”

O estado da arte da cromatografia líquida de ultra eficiência

“…Além dos trabalhos descritos acima, outros foram realizados empregando CLUE, como, por exemplo, determinação de lactonas, 23 to- xinas marinhas, 45 cianobactérias, 46 drogas veterinárias, 47 saponinas, 12,48 enzimas 49 e metabonomas, 50 o que comprova que a cromatografia líquida de ultra eficiência vem sendo avaliada e empregada em diversas áreas.…”